Structure mapping of molecular complexes by super-resolution microscopy

通过超分辨率显微镜绘制分子复合物的结构图

• 批准号: 9902489
• 负责人: Bo Huang
• 金额: $ 34.28万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-05-04 至 2022-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9902489
• 关键词: Address; Affect; Algorithmic Analysis; Algorithms; Antibodies; Architecture; Binding; Biochemical; Biological Models; Biological Process; Biology; Cells; Centrosome; Chimeric Proteins; Cilia; Collaborations; Complex; Cryoelectron Microscopy; Crystallography; Data; Development; Diffusion; Dimensions; Disease; Epitopes; Explosion; Face; Fluorescence; Fluorescence Microscopy; Fluorescent Probes; Focal Adhesions; Gene Mutation; Genes; Genetic; Heterogeneity; Image; Image Analysis; Immunofluorescence Immunologic; In Vitro; Individual; Label; Macromolecular Complexes; Maps; Methods; Microscope; Microscopy; Modernization; Molecular; Molecular Conformation; Morphologic artifacts; Morphology; Mutation; Nuclear Magnetic Resonance; Nuclear Pore Complex; Pathogenesis; Positioning Attribute; Process; Proteins; Regulation; Resolution; Ribosomes; Role; Signal Transduction; Structure; Structure-Activity Relationship; Techniques; Technology; Tertiary Protein Structure; Testing; Transportation; X-Ray Crystallography; base; ciliopathy; computerized tools; experience; experimental study; flexibility; fluorophore; image registration; improved; insight; kinetosome; macromolecular assembly; macromolecule; novel therapeutics; organizational structure; overexpression; particle; protein transport; quantitative imaging; reconstruction; stoichiometry; structural biology; three dimensional structure; trafficking

PROJECT SUMMARY Eluciding the structural organization of macromolecules or molecular complexes is one of the fundamental steps towards mechanistic understanding of their function and activity regulation. With atomic level details elucided by traditional structural biology techniques such as crystallography, nuclear magnetic resonance and cryo-electron microscopy, the next step is to place this structural information in to the cellular context. For this purpose, we take the approach of using super-resolution fluorescence microscopy to map the spatial coordinates of their individual components. For this approach, we plan to develop a scalable method for efficient alabeling endogenous proteins for super-resolution microscopy, as well as analysis algorithms for super-resolution images. We will apply this approach to the study of basal body - cilium transition zone, which has been shown in our preliminary study to be a gate structure that regulates protein trafficking in the cilium.

项目总结