Technology Core

通过本技术核心中的活动，我们将提供全面的高通量基因组 测序和分析的研究人员参与这一U19的建议，以推进研究在所有四个 这些项目的重点是了解宿主，病原体和微生物组相互作用作为疾病的决定因素。 传染病的结果。我们将提供标准的高通量基因组，转录组，宏基因组， metatranscriptome和单细胞测序以及我们所需的其他标准测序方法。 病毒，细菌，真菌和寄生虫研究项目和任何未来的合作试点项目。这 包括Illumina、Pacific Biosciences和Oxford等主要测序平台上的测序 纳米孔。当传染病研究时，我们还将提供定制测序方法 这是必要的，基于我们在多微生物样品测序和分析方面的丰富经验。我们将 使用最新的测序和分析工具来改进基因组注释，为 传染病社区。我们将把研究和发展活动的重点放在发展一个 强大的单细胞转录组学，用于分析宿主-病原体样品，包括单细胞 缺乏多聚腺苷酸化转录物的原核病原体的转录组学。鼓励和支持 项目中描述的基因组编辑方法的实施和分析。最后， 我们将整合我们的技术和数据核心，以无缝地将测序数据和新的 分析工具中生成的技术核心到数据核心。

Through the activities in this Technology Core, we will provide comprehensive high-throughput genome sequencing and analysis to the investigators participating in this U19 proposal to advance research in all four projects that are focused on understanding host, pathogen, and microbiome interactions as determinants of infectious disease outcome. We will provide standard high throughput genome, transcriptome, metagenome, metatranscriptome, and single cell sequencing as well as other standard sequencing methods required by our Viral, Bacterial, Fungal, and Parasite research projects and any future collaborative pilot projects. This includes sequencing on every major sequencing platform including Illumina, Pacific Biosciences, and Oxford Nanopore. We will also provide custom sequencing approaches for the study of infectious disease, when necessary, building on our immense experience on sequence and analysis of polymicrobial samples. We will use the latest sequencing and analysis tools to improve genome annotation, providing an important resource to the infectious disease community. We will focus our research and development activities on developing a robust single cell transcriptomics for the analysis of host-pathogen samples, including single cell transcriptomics on prokaryotic pathogens lacking polyadenylated transcripts. We will encourage and support the implementation and analysis of genome editing approaches that are described within the projects. Lastly, we will integrate our Technology and Data Cores to seamlessly transition sequencing data and novel analysis tools generated in the Technology Core to the Data Core.