Technology Core

通过这个技术核心的活动，我们将提供全面的高通量基因组 对参与这项U19提案的研究人员进行测序和分析，以推进所有四项研究 专注于了解宿主、病原体和微生物群相互作用作为决定因素的项目 传染病结局。我们将提供标准的高通量基因组、转录组、元基因组、 和单细胞测序以及我们所需的其他标准测序方法 病毒、细菌、真菌和寄生虫研究项目以及未来的任何合作试点项目。这 包括在每个主要测序平台上进行测序，包括Illumina、Pacific Biosciences和Oxford 纳米孔。我们还将为传染病的研究提供定制的测序方法，当 根据我们在多个微生物样本的测序和分析方面的丰富经验，这是必要的。我们会 使用最新的测序和分析工具改进基因组注释，为以下方面提供重要资源 传染病社区。我们将把研发活动的重点放在开发一种 用于包括单细胞在内的寄主病原体样本分析的强健的单细胞转录切割技术 原核病原体缺乏多聚腺苷化转录本的转录组学。我们将鼓励和支持 项目中描述的基因组编辑方法的实施和分析。最后， 我们将整合我们的技术和数据核心，以无缝转换测序数据和新颖的 从技术核心到数据核心生成的分析工具。

Through the activities in this Technology Core, we will provide comprehensive high-throughput genome sequencing and analysis to the investigators participating in this U19 proposal to advance research in all four projects that are focused on understanding host, pathogen, and microbiome interactions as determinants of infectious disease outcome. We will provide standard high throughput genome, transcriptome, metagenome, metatranscriptome, and single cell sequencing as well as other standard sequencing methods required by our Viral, Bacterial, Fungal, and Parasite research projects and any future collaborative pilot projects. This includes sequencing on every major sequencing platform including Illumina, Pacific Biosciences, and Oxford Nanopore. We will also provide custom sequencing approaches for the study of infectious disease, when necessary, building on our immense experience on sequence and analysis of polymicrobial samples. We will use the latest sequencing and analysis tools to improve genome annotation, providing an important resource to the infectious disease community. We will focus our research and development activities on developing a robust single cell transcriptomics for the analysis of host-pathogen samples, including single cell transcriptomics on prokaryotic pathogens lacking polyadenylated transcripts. We will encourage and support the implementation and analysis of genome editing approaches that are described within the projects. Lastly, we will integrate our Technology and Data Cores to seamlessly transition sequencing data and novel analysis tools generated in the Technology Core to the Data Core.