Environmental Epigenetics and Molecular Analyses Facility Core

项目总结 环境表观遗传学和分子分析设施核心(EEMAFC)的总体目标是 为中心成员提供使用最先进工具评估效果的机会和教育 环境暴露对人类和其他组织细胞、组织和器官的表观基因组和基因组的影响 物种。越来越明显的是，表观基因组在调节细胞周期中起着巨大的作用 生物体对各种环境暴露的反应。因此，已经实施的新倡议 由于上次更新包括现在常规用于转录组和染色质的RNA-Seq和ChIP-Seq 研究对环境的毒性和疾病反应的潜在机制的状态发现 探员们。除了扩展的表观遗传学服务，核心还继续提供基本的分子， 显微镜和分析服务，支持正在进行的表观遗传学、基因组学、基因表达和 该中心的信号通路研究。这些服务包括基本的分子和分析服务，包括新的 例如，用于高通量定量聚合酶链式反应的设备，以及用于细胞和组织中金属分析的新设备 对于暴露的生物，因为金属对表观基因组、基因组和细胞功能的影响是专门的 纽约大学NIEHS中心的研究优势。这一核心不断演变的基本原理反映了 环境健康研究的当前趋势，特别是迅速变化的实地调查 环境对表观基因组/基因组的影响。

PROJECT SUMMARY The overall objective of the Environmental Epigenetics and Molecular Analyses Facility Core (EEMAFC) is to provide Center members with access to, and education on using, state-of-the-art tools for assessing the effects of environment exposures on the epigenomes and genomes of cells, tissues and organs of humans and other species. It has become increasingly evident that the epigenome plays an enormous role in modulating the responses of organisms to myriad environmental exposures. Thus, new initiatives that have been implemented since the last renewal include the now routinely used RNA-Seq and ChIP-Seq for transcriptome and chromatin status discovery for investigating mechanisms underlying toxic and disease responses to environmental agents. In addition to the expanded epigenetics services, the Core also continues to provide basic molecular, microscopy and analytical services in support of ongoing epigenetics, genomics, gene expression, and signaling pathway research in the Center. These include basic molecular and analytical services, including new equipment for high throughput qPCR, for example, and new equipment for metals analysis in cells and tissues of exposed organisms, since metal effects on the epigenome, genome and cell functions are specialized research strengths of the NYU NIEHS Center. The rationale for the continuing evolution of this Core reflects current trends in environmental health research, in particular the rapidly changing field investigating environment interactions on epigenomes/genomes.