Improving the clarification, characterization, and homogeneity of animal serum through Nanotrap nanoparticle technology

通过 Nanotrap 纳米颗粒技术提高动物血清的澄清度、表征和均质性

• 批准号: 9906315
• 负责人: Benjamin Scott Lepene
• 金额: $ 22.49万
• 依托单位: CERES NANOSCIENCES, LLLP
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-02-01 至 2021-05-17
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9906315
• 关键词: Acids; Address; Adult; Affect; Affinity; Animals; Antibodies; Area; Bacteria; Binding; Biological; Birth; Cattle; Cell Culture Techniques; Cell Line; Cell Proliferation; Cellular biology; Centrifugation; Chemicals; Coagulation Process; Collection; Complex; Culture Media; Data; Detection; Endotoxins; Environment; Filtration; Funding; Growth; Growth Factor; Hormones; Human; Hydrogels; Individual; Industry; International; Investigation; Liquid substance; Methods; Microspheres; Mycoplasma; Performance; Phase; Prions; Process; Proteins; Quality Control; Reagent; Reproducibility; Research; Resources; Safety; Sales; Sampling; Science; Serum; Small Business Innovation Research Grant; Sterility; Structure; Supplementation; System; Technology; Testing; Time; Vendor; Viral; Virus; Whole Blood; Work; base; cell growth; cost; experimental study; extracellular vesicles; fetal bovine serum; improved; innovation; molecular sieving; nanoparticle; nanoscience; operation; particle; pathogen; precision medicine; quality assurance; response; screening; success; tissue culture; tool

PROJECT SUMMARY Cell culture techniques have, and will continue to, significantly contribute to many of areas of biomedical science and research that will benefit humans and animals. In most cases, the supplementation of minimal culture media with animal-derived products, e.g., animal serum, is essential for proper cell growth. Serum is the centrifuged fluid component of either clotted or defibrinated whole blood taken from domestic cattle. It provides hormone factors for cell growth and proliferation and provides all of the essential factors needed for maintaining a favorable growth environment. Among several common animal sera, fetal bovine serum (FBS) has been the most widely used and is viewed as a critical reagent for the success of current scientific research and discovery. Despite its widespread use, FBS has a number of well-appreciated problems; it is heterogeneous, with great variety from lot to lot, and is frequently contaminated with things like endotoxins, mycoplasma, bacteria, and/or viruses. Variability and contamination significantly affect the reproducibility of experiments and the safety of products. Better quality control methods are needed to eliminate and detect the presence of serum contaminants, especially from large batches of serum and in a high-throughput manner. In response to this need, Ceres Nanosciences, Inc. has devised a solution leveraging its innovative and proprietary Nanotrap technology, which consists of customizable hydrogel microspheres that have widespread applicability in other fields for separating, targeting, stabilizing, and enriching target analytes from biofluids. Based on supporting proof-of-concept data and through three specific aims, Ceres proposes a Phase I SBIR to evaluate the feasibility of using Nanotrap particles to improve the extraction and detection of common contaminants from serum, and to investigate the integration of Nanotrap technology into a scalable, higher-throughput workflow that is able to be used with large- batch volumes of serum. If successful, the application of this technology could significantly impact the way serum is produced by: accelerating the processing time needed to satisfy regulatory requirements; improving the ability to detect and remove contaminants; and streamlining the workflow between serum collection and sale. This work would then also improve the overall quality of serum being used by the industry, thus helping to improve this essential resource for all applications of cell and tissue culture.

项目总结 细胞培养技术已经并将继续对生物医学的许多领域做出重大贡献。 以及将造福人类和动物的研究。在大多数情况下，补充最低限度的培养基时 使用动物衍生产品，例如动物血清，对细胞的正常生长至关重要。血清是经离心分离的 取自家牛的凝结或去纤化的全血的液体成分。它提供荷尔蒙 细胞生长和增殖的因子，并提供维持良好的 成长环境。在几种常见的动物血清中，胎牛血清(FBS)应用最为广泛 使用并被视为当前科学研究和发现成功的关键试剂。尽管它的 广泛使用，FBS有许多公认的问题；它是异构性的，从 多种多样，经常受到内毒素、支原体、细菌和/或病毒等物质的污染。 可变性和污染严重影响实验的重现性和产品的安全性。 需要更好的质量控制方法来消除和检测血清污染物的存在， 尤其是从大批量的血清中以高通量的方式。为了回应这一需求，谷神星 NanoSciences，Inc.利用其创新和专有的NanoTrap技术设计了一种解决方案， 由可定制的水凝胶微球组成，这种微球在其他领域具有广泛的适用性， 从生物体液中定位、稳定和富集靶向分析物。基于支持概念验证数据 通过三个具体目标，CERES提出了一种第一阶段的SBIR来评估使用NanoTrap的可行性 以改进从血清中提取和检测常见污染物的方法，并研究 将NanoTrap技术集成到可扩展、吞吐量更高的工作流中，该工作流能够用于大型 批量的血清。如果成功，这项技术的应用可能会对血清产生重大影响 产生于：加快满足监管要求所需的处理时间；提高能力 检测和清除污染物；以及简化血清收集和销售之间的工作流程。这部作品 还将提高该行业使用的血清的整体质量，从而有助于改善这一点 对于细胞和组织培养的所有应用来说都是必不可少的资源。