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Pathophysiological Regulation of Atrial Alternans and Atrial Fibrillation

心房交替和心房颤动的病理生理调节

基本信息

批准号：
9907864
负责人：
LOTHAR A BLATTER
金额：
$ 38.75万
依托单位：
RUSH UNIVERSITY MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-04-01 至 2022-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9907864
关键词：
3-Dimensional Action Potentials Arrhythmia Atrial Fibrillation Calcium Cardiac Cell Communication Cell model Cells Chlorides Complex Computer Models Computer Simulation Coupling Disease Ectopic beats Electrophysiology (science)Etiology Event Feedback Frequencies Generations Goals Heart Heart Atrium Heart Diseases Heart failure Heterogeneity Ice Image Inositol Intracellular Signaling Proteins Left Link Mediating Membrane Mitochondria Modeling Muscle Cells Organ Oryctolagus cuniculus Pharmacology Play Predisposition Probability Property Proteins Refractory Regulation Risk Risk Factors Role Sarcoplasmic Reticulum Severities Signal Pathway Signal Transduction Source Surface System Testing Therapeutic Therapeutic Intervention Time Tissues Tubular formation Up-Regulation Validation Ventricular design intercellular communication novel pressure receptor spatiotemporal sudden cardiac death targeted treatment theories therapy design uptake voltage

项目摘要

PROJECT SUMMARY/ABSTRACT Atrial fibrillation (AF), the most common form of cardiac arrhythmia, is preceded by episodes of alternans in the atrium. These beat-to-beat alternations in action potential (AP) duration, contraction strength and Ca transient (CaT) amplitude form a dynamic AF substrate by creating temporal and spatial heterogeneity of electrical tissue properties and Ca signaling. Heart failure (HF) induced atrial remodeling changes the expression and regulation of key Ca handling proteins thereby promoting profound changes of excitation-contraction coupling (ECC) that further increase susceptibility to atrial arrhythmogenic Ca release and alternans. Due to the lack or paucity of a transverse tubular system atrial ECC reveals unique features that are strikingly different from ventricular myocytes and make atrial cells especially prone to develop alternans. The bidirectional coupling of membrane voltage (Vm) and [Ca]i regulation (Vm↔[Ca]i coupling) creates complex feedback mechanisms that play a pivotal role for the generation of alternans. Therefore, the overall goal of this proposal is to establish an experimentally tested mechanistic model of atrial alternans and to establish a mechanistic link between atrial alternans, atrial remodeling in HF and AF at the cellular, multicellular and whole heart level. Specific aim 1. Identify the cellular mechanisms of electrical (AP duration, APD) and CaT alternans in atrial myocytes. We will test the hypothesis that disturbances of atrial Ca signaling during ECC (sarcoplasmic reticulum (SR) Ca load hypothesis vs. refractoriness hypothesis) are the primary cause of alternans and through the regulation of Ca-dependent membrane conductances (voltage-gated L-type Ca, Na/Ca exchange, Ca-dependent chloride and small conductance Ca-activated K currents) Ca alternans determines electrical APD alternans and increase the propensity of proarrhythmic Ca release events. Specific aim 2. Identify the HF remodeling attributes that enhance atrial alternans propensity. We will test the hypothesis that atrial Ca signaling proteins and pathways as well as the ECC mechanism undergo profound remodeling in HF that result in a higher propensity of atrial alternans. In a rabbit left-ventricular HF model we will further test how enhanced IP3 receptor-mediated Ca release, increased SR Ca leak and remodeled mitochondrial Cauptake facilitates the probability of atrial alternans. Specific aim 3. Establish a mechanistic causation linking atrial alternans and AF. Tissue arrhythmia (AF) requires cell-to-cell communication, an arrhythmogenic focus (ectopic activity) and transient or permanent tissue inhomogeneity (conduction heterogeneity). In Langendorff perfused hearts (normal and HF) the spatio- temporal properties of tissue-wide APD and CaT alternans and the relationship to AF inducibility will be investigated by atrial bipolar electrograms, multielectrode surface mapping and Ca imaging. In cell pairs cell-to- cell communication mechanism underlying alternans will be investigated. Therapeutic strategies will be developed to curtail the increased risk of alternans and AF in HF.

项目总结/文摘