Capacity for CARB WGS Surveillance at Cornell AHDC

项目概要/摘要 越来越需要监测动物中的抗菌素耐药性，以保护药物的安全性。 人和动物在兽医诊断实验室进行经认证的细菌全基因组测序的能力 支持FDA的使命，确保我国食品供应的安全，保护公众健康， 从而能够实时监测分离株。在本提案中，我们要求提供资金， 获得FDA认可的细菌测序服务。执行高级分子生物学的能力 以可靠的方式表征细菌分离株对我们快速应对疑似 微生物污染和监测动物和饲料中的抗菌素耐药性。重要的是， 兽医诊断实验室的这一能力填补了一个关键的监测空白， 抗菌素耐药性除了作为序列参考实验室之一，古德曼实验室 在康奈尔大学提供了分析支持，以计划办公室和指导其他实验室， 为这些程序提供验证、方法和质量保证文件，以支持 ISO：17025认证。拟议的研究将对人类和动物健康产生重要影响 并将提高Vet-LIRN对食源性病原体进行有效监测的能力。

Project Summary/Abstract There is a growing need for monitoring antimicrobial resistance in animals in order to protect the safety of people and animals. Capacity for accredited bacterial whole genome sequencing in veterinary diagnostic labs supports the mission of FDA to ensure the safety of our nation's food supply and protect public health by enabling real-time monitoring of isolates. In this proposal, we request funding to maintain capacity for our accredited bacterial sequencing services for FDA. The capacity to perform advanced molecular characterization of bacterial isolates in a reliable manner is critical to our ability to respond quickly to suspected microbiological contamination and monitor antimicrobial resistance in animals and feed. Importantly, having this capacity in veterinary diagnostic laboratories fills a critical surveillance gap by monitoring live animals for antimicrobial resistance. In addition to serving as one of the sequence reference labs, the Goodman laboratory at Cornell has provided analytic support to the program office and mentorship to the other laboratories by providing validation, methods, and Quality Assurance documentation for these procedures in support of ISO:17025 accreditation. The proposed studies will have important impacts for both human and animal health and will improve the capabilities of Vet-LIRN to perform effective surveillance for food-borne pathogens.