Adapting molecular food safety tests to alternative matrices

使分子食品安全测试适应替代基质

• 批准号: 8828925
• 负责人: Laura Brunengraber Goodman
• 金额: $ 9.83万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2019-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8828925
• 关键词: Adapting; molecular; food; safety; tests

Project Summary/Abstract Monitoring for pathogens causing enteric diseases such as salmonellosis is needed in a number of sectors including food and feed production, retail facilities, and veterinary hospitals. Several commercially available kits are available for this purpose, but are validated primarily for human food testing. A comprehensive monitoring plan for food production facilities should include the ability to test multiple types of samples, including tissues and feces from sick or dead animals and/or rodents and environmental swabs. Validation of rapid assays for additional sample types such as animal tissues, fecal samples, and environmental swabs is urgently needed as part of comprehensive surveillance efforts and outbreak management plans. There is a large demand for rapid screening for enteric bacterial pathogens such as Salmonella spp. in a number of sectors including food and feed production facilities. Several commercially available kits are available for this purpose, but are AOAC validated only for human food testing. Validation of rapid assays for additional matrices such as animal tissues, fecal samples, and environmental surface swabs is urgently needed. In order to accomplish this, we propose the following specific aims: 1) Validate commercially available molecular diagnostic assays for Salmonella spp., E. coli, and Listeria spp. for use in fecal samples, animal tissues, and environmental swabs; 2) Transfer these assays to an open array platform in order to facilitate testing efficiency and transferability to other laboratories. Extending validations of rapid food testing kits for additional animal-related matrices, which is feasible due to our expertise in veterinary diagnostics, will have direct relevance to public health by providing new methods for comprehensive enteric pathogen surveillance and outbreak management. The long-term goal of the proposed research is to facilitate the dissemination of high-throughput, cost-effective methods for screening of multiple sample types for a broad range of enteric pathogens.

项目摘要/摘要 许多人需要监测引起肠道疾病的病原体，如沙门氏菌病。 这些部门包括食品和饲料生产、零售设施和兽医医院。几个 商用试剂盒可用于此目的，但主要针对人类进行验证 食品检测。对粮食生产设施的全面监测计划应包括 能够检测多种类型的样本，包括患病或死亡动物的组织和粪便 和/或啮齿动物和环境拭子。对其他样本类型的快速检测的验证 例如动物组织、粪便样本和环境拭子，作为 全面的监测工作和疫情管理计划。 对沙门氏菌等肠道细菌病原体的快速筛查需求很大 SPP.在包括食品和饲料生产设施在内的多个部门。几个商业上的 可用的试剂盒可用于这一目的，但AOAC仅对人类食品测试进行验证。 对其他基质，如动物组织、粪便样本和 环境表面拭子是迫切需要的。为了实现这一目标，我们建议 以下具体目标：1)验证商业上可用的分子诊断分析 沙门氏菌、大肠杆菌、李斯特氏菌。用于粪便样本、动物组织和 环境拭子；2)将这些分析转移到开放阵列平台，以便于 测试效率和可移植到其他实验室。 延长额外动物相关基质食品快速检测试剂盒的有效性，这是 由于我们在兽医诊断方面的专业知识是可行的，将与公共卫生直接相关 通过为全面的肠道病原体监测和暴发提供新的方法 管理层。拟议研究的长期目标是促进传播 用于筛选多种样本类型的高通量、经济高效的方法 肠道病原体的范围。