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Flavivirus cellular tropisms driving dissemination and transmission in mosquito vectors

黄病毒细胞向性驱动蚊媒传播和传播

基本信息

批准号：
9911055
负责人：
Kendra Marie Quicke
金额：
$ 6.49万
依托单位：
COLORADO STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-02-01 至 2023-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9911055
关键词：
Address Aedes Affect Animal Model Area Automobile Driving Bite Brain Case Fatality Rates Cells Cellular Tropism Centers for Disease Control and Prevention (U.S.)Characteristics Clone Cells Colorado Congenital Abnormality Culicidae Cytomegalovirus Data Dendritic Cells Dengue Hemorrhagic Fever Dengue Infection Dengue Shock Syndrome Dengue Virus Development Disease Educational workshop Effectiveness Endothelial Cells Engineering Epidemic Extravasation Feeding behaviors Fellowship Fetal Development Flavivirus Flavivirus Infections Foundations Genome Grant Head Hemocytes Icterus Immune Individual Infection Insect Vectors Institution Knowledge Laboratories Liquid substance Liver MicroRNAs Microcephaly Midgut Multiple Organ Failure Mus Neurons Neurotropism Organ Pathology Population Predisposition Public Health Reagent Research Research Training Risk Role Salivary Glands System Techniques Testing Tissues Training Training Activity Tropism Universities Vascular Permeabilities Viral Viral Pathogenesis Virus Virus Diseases Virus Replication Work Writing Yellow Fever Yellow fever virus Zika Virus career cell type data tools differential expression experimental study feeding human model improved interest lymph nodes macrophage miRNA expression profiling mosquito-borne neonatal infection nerve stem cell next generation sequencing post-doctoral training responsible research conduct skills success symposium tissue tropism tool transmission process vector vector competence vector mosquito viral transmission

项目摘要

Project Summary/Abstract The research objectives of this fellowship are to determine the cellular tropisms of three mosquito-borne flaviviruses of public health importance – Zika virus, dengue virus and yellow fever virus – within their Aedes aegypti vectors and to define the roles of specific cell populations in viral dissemination and transmission. The aims are to 1) identify the specific cell populations critical for viral dissemination from the midgut to the salivary glands, and 2) define the impact of specific tissue tropisms on mosquito feeding behavior and viral transmission. Aim 1 will be accomplished by generating microRNA (miRNA) expression profiles for midgut, hemocyte and neuronal cell populations and selecting miRNAs that are highly differentially expressed in each population. We will confirm infection of cells expressing these miRNAs and generate cell-specific, miRNA-restricted viruses containing target sequences for cell-specific miRNAs to probe the roles of each cell type in viral dissemination. Aim 2 will be accomplished by using miRNA-restricted viruses to determine the effect of neuronal cell and salivary gland infection on mosquito feeding behaviors and subsequent transmission of the virus by observing infected mosquitoes feeding on mice and testing mice for the presence of virus. The data and tools produced by this work could aid the development of transmission control strategies that target viral replication in specific cell populations within the vector. Research and postdoctoral training activities will be undertaken at both Colorado State University and the Centers for Disease Control and Prevention in Fort Collins, CO. The sponsor and co- sponsor laboratories have complimentary research interests and facilities that will promote the success of this project. In addition to research, the training plan includes completion of coursework in next generation sequencing experiment and analysis, grant writing, and responsible conduct of research; participation in supervisory training; and attendance at various workshops, seminars and conferences to improve writing, presentation and career building skills.

项目总结/文摘