Unbiased visualization of human ASC subsets
Unbiased visualization of human ASC subsets
批准号:
9916109
负责人:
Lisa Borghesi
金额:
$18.86万
依托单位国家:
美国
项目类别:
财政年份:
2020
资助国家:
美国
项目状态:
已结题
起止时间:
2020-01-01 至 2021-12-31
关键词:
AntibodiesAntibody RepertoireB-LymphocytesBiological AssayBloodCell physiologyCharacteristicsClone CellsColonComplexComputational algorithmDataDiseaseDrug or chemical Tissue DistributionEnvironmentExposure toFlow CytometryGenetic TranscriptionGut MucosaHumanHuman bodyHumoral ImmunitiesImmune responseImmunoglobulin-Secreting CellsImmunologicsIn VitroIndividualInfection ControlInguinal lymph node groupIntestinesKnowledgeLeadLinkLongevityLungLymphoid TissueMaintenanceMapsMediator of activation proteinMucous MembraneNormal tissue morphologyOutcomes ResearchPathway interactionsPhenotypePhosphoproteinsPlasma CellsPopulationProtocols documentationPublishingSerumSignal TransductionSiteStructureSurfaceTestingTissuesVaccinesValidationVisualizationdata visualizationdifferential expressiondraining lymph nodehigh dimensionalityhuman tissueileumimmunoregulationimprovedinsightjejunummesenteric lymph nodemicrobialnext generationnovelpathogenprogramsprotein expressionresidencestemtissue resourcetranscription factortranscriptome sequencing
中文摘要
摘要:
浆细胞(PC)负责维持血清中病原体抗体水平。但
缺乏人体组织区室中PC亚群的综合空间图。作为
因此,我们对个体内不同组织的全球PC群体结构的理解
不同的个体之间也是模糊的。使用独特和稀有的人体组织资源
沿着高维流式细胞术,将检验PC亚群组成可以被
根据组织部位预测,长寿命PC(LLPC)参与针对每个部位的生存计划
混元在目标1中,我们将从正常的淋巴和粘膜组织中鉴定已知的和新的PC亚群,
使用复杂的流式细胞术和无偏计算算法的人供体。我们将整合
表型与信号状态和建立PC功能能力。在目标2中,我们将执行Next
对来自两种不同组织的长寿命PC(LLPC)进行RNA测序和功能测定
位点,以揭示共享与组织特异性生存途径。这项研究的结果将揭示
负责人类体液免疫的PC亚群的全球人口结构,并提供对
PC如何控制区域和系统感染。
英文摘要
Abstract:
Plasma cells (PC) are responsible for maintaining serum antibody levels to pathogen. However, a
comprehensive spatial map of PC subsets across tissue compartments in the human body is lacking. As a
consequence, our understanding of global PC population structure across different tissues within individuals
and also between different individuals is murky. Using unique and rare-to-access human tissue resources
along with high-dimensional flow cytometry, will test the hypothesis that PC subset composition can be
predicted by tissue site and that long-lived PC (LLPC) engage survival programs that are tailored to each site
of origin. In Aim 1, we will identify known and novel PC subsets in lymphoid and mucosal tissues from normal
human donors using complex flow cytometry and unbiased computational algorithms. We will integrate
phenotype with signaling status and establish PC functional capabilities. In Aim 2, we will perform Next
Generation RNA-Sequencing and functional assays on long-lived PCs (LLPC) derived from two distinct tissue
sites in order to uncover shared versus tissue-specific survival pathways. Results from this study will reveal the
global population structure of PC subsets responsible for human humoral immunity and provide insights into
how PCs control infections regionally and systemically.
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会议论文
ZellScanner ONE
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批准号:10176806
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项目类别:
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资助金额:$28.68万
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财政年份:2021
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负责人:Lisa Borghesi
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依托单位:
B cell population structure in mouse and man
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批准号:10225407
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项目类别:
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资助金额:$38.67万
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财政年份:2019
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负责人:Lisa Borghesi
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依托单位:
B cell population structure in mouse and man
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批准号:9753465
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项目类别:
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资助金额:$38.67万
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财政年份:2019
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负责人:Lisa Borghesi
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依托单位:
TLR4 shapes BM HSCs and lymphopoiesis
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批准号:9316026
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项目类别:
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资助金额:$19.25万
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财政年份:2017
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负责人:Lisa Borghesi
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依托单位:
Mechanisms of TLR4-mediated impairment of murine and human HSC function
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批准号:8635494
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项目类别:
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资助金额:$19.76万
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财政年份:2013
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负责人:Lisa Borghesi
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依托单位:
Mechanisms of TLR4-mediated impairment of murine and human HSC function
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批准号:8776705
-
项目类别:
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资助金额:$23.37万
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财政年份:2013
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负责人:Lisa Borghesi
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依托单位:
Special BD LSRFortessaTM
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批准号:8244704
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项目类别:
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资助金额:$38.35万
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财政年份:2012
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负责人:Lisa Borghesi
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依托单位:
The role of E47 in uncommitted hematopoietic progenitors
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批准号:8431442
-
项目类别:
-
资助金额:$30.91万
-
财政年份:2009
-
负责人:Lisa Borghesi
-
依托单位:
The role of E47 in uncommitted hematopoietic progenitors
-
批准号:7776945
-
项目类别:
-
资助金额:$33.21万
-
财政年份:2009
-
负责人:Lisa Borghesi
-
依托单位:
The role of E47 in uncommitted hematopoietic progenitors
-
批准号:7647823
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项目类别:
-
资助金额:$33.2万
-
财政年份:2009
-
负责人:Lisa Borghesi
-
依托单位:
The role of E47 in uncommitted hematopoietic progenitors
-
批准号:8026003
-
项目类别:
-
资助金额:$32.88万
-
财政年份:2009
-
负责人:Lisa Borghesi
-
依托单位:
The role of E47 in uncommitted hematopoietic progenitors
-
批准号:8225346
-
项目类别:
-
资助金额:$32.88万
-
财政年份:2009
-
负责人:Lisa Borghesi
-
依托单位:
Cellular and molecular mechanisms of HSC dysfunction in chronic inflammation
-
批准号:9107580
-
项目类别:
-
资助金额:$37.76万
-
财政年份:2009
-
负责人:Lisa Borghesi
-
依托单位:
Clonal analysis of joint-infiltrating natural Killer cells in chronic arthritis
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批准号:7446093
-
项目类别:
-
资助金额:$7.11万
-
财政年份:2007
-
负责人:Lisa Borghesi
-
依托单位:
Clonal analysis of joint-infiltrating natural Killer cells in chronic arthritis
-
批准号:7622552
-
项目类别:
-
资助金额:$7.11万
-
财政年份:2007
-
负责人:Lisa Borghesi
-
依托单位:
Clonal analysis of joint-infiltrating natural Killer cells in chronic arthritis
-
批准号:7193869
-
项目类别:
-
资助金额:$7.0万
-
财政年份:2007
-
负责人:Lisa Borghesi
-
依托单位:
海外基金