B cell population structure in mouse and man

摘要： B细胞负责维持体液免疫。然而，一张全面的小鼠空间地图 而人体内跨组织隔间的B细胞亚群缺乏。这些研究的一个障碍是 缺乏对来自许多不同组织和组织的大量细胞进行全面分析的方法 还可以获得稀有的人类标本。在这里，我们使用先进的计算和公正的方法 组织小鼠和人的B细胞亚群及其信号转导特性。我们将检验B细胞的假设 子集组成可以通过组织部位来预测，不同组织部位中长寿命PC具有 不同的监管方案反映了它们独特的微环境。在目标1中，我们将建立 使用无偏见的方法研究小鼠和人类B血统亚群在全身的种群结构。 在目标2中，我们将建立小鼠长寿的组织特异性调控程序和克隆关系 PC(LLPC)来源于两种不同的微环境，BM和Gut。这项研究的结果将揭示 负责体液免疫的B亚群的全球种群结构并提供了对B细胞如何 有区域、有系统地控制感染。

Abstract: B cells are responsible for maintaining humoral immunity. However, a comprehensive spatial map of murine and human B cell subsets across tissue compartments in the body is lacking. A barrier to these studies has been the lack of methods for comprehensive analysis of large numbers of cells from many different tissues and also access to rare human specimens. Here we use advanced computing and unbiased approaches to organize B cell subset and their signaling properties in mouse and man. We will test the hypothesis that B cell subset composition can be predicted by tissue site and that long-lived PCs in different tissues sites have distinct regulatory programs that reflect their unique microenvironments. In Aim 1, we will establish the population structure of murine and human B lineage subsets throughout the body using unbiased approaches. In Aim 2, we will establish the tissue-specific regulatory programs and clonal relationships of murine long-lived PC (LLPC) derived from two distinct microenvironments, BM versus gut. Results from this study will reveal the global population structure of B subsets responsible for humoral immunity and provide insights into how B cells control infections regionally and systemically.