ZellScanner ONE

泽尔扫描仪一号

• 批准号: 10176806
• 负责人: Lisa Borghesi
• 金额: $ 28.68万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-05-01 至 2022-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10176806
• 关键词: Architecture; Area; Biological Sciences; Biology; Blood Vessels; Caring; Cells; Direct Costs; Discipline; Ensure; Flow Cytometry; Funding; Hour; Human Resources; Image; Immune; Immune System Diseases; Immunology; Individual; Infrastructure; Institutes; Microscopy; Minor; Oncology; Phase; Positioning Attribute; Productivity; Research; Research Personnel; Rheumatology; Sister; System; Time; Tissues; Training; Transplantation; Universities; Work; cohort; experience; experimental study; imaging platform; insight; instrument; medical schools

Project Summary/Abstract This request is for funds to purchase a Canopy Biosciences ZellScanner One high-multiplexing chip cytometer. There is no ZellScanner imaging platform at the University of Pittsburgh or within 100 miles. Unique as an instrument, the ZellScanner system combines the strengths of multiparameter flow cytometry (marker co-expression, quantitative, sensitive) with microscopy (positional information, tissue architecture, subcellular detail) to permit analysis of highly defined immune subsets and their spatial positioning in the native tissue microenvironment. Such high content imaging allows for deep insights into immune cell subpopulations in the context of tissue localization. The instrument will be housed within the Unified Flow Cytometry Core, a centralized core, at the University of Pittsburgh School of Medicine. The Unified Flow Core currently serves over 180 Pis and 500 individual Users. For the proposed instrument, Major Users include 13 Pis (10 NiH funded) with another 5 Pis as Minor Users. Together, these Pis have over $20 M in NiH-funded direct costs from at least 4 different institutes. A major research focus is in the broad area of immunology and allied immune diseases, with sister disciplines such as transplantation, rheumatology, vascular biology, and oncology also well represented. While the Unified Flow Cytometry Core is well-endowed with traditional (though busy) analyzers and sorters, the ZellScanner chip cytometer brings totally new breadth to our research capabilities. Our preliminary and demo work proved to us that this instrument will truly allow our investigators to break new ground in their research. The demo phase of this instrument indeed created great excitement among the Pis and hands-on Users. The new machine will be well cared for by the staff of the Unified Flow Cytometry Core, which has an extensive infrastructure and culture in place to train Users, oversee the machine and ensure that it is self-supporting. Two experienced core personnel will be responsible for the conceptual and day to day aspects of experiments on the ZellScanner unit. We will start and certify the machine each morning and troubleshoot any problems, as we will do at any time during working hours. As such, it will have a remarkable impact on the productivity of an outstanding and well-funded cohort of PHS investigators.

项目总结/摘要 该请求是为了购买Canopy Biosciences ZellScanner One高复用芯片的资金 细胞计数器。匹兹堡大学或100个以内没有ZellScanner成像平台 英里作为一种独特的仪器，ZellScanner系统结合了 多参数流式细胞术（标记物共表达，定量，灵敏）， 显微镜（位置信息，组织结构，亚细胞细节），以允许分析 高度确定的免疫亚群及其在天然组织中的空间定位 微环境这种高内涵的成像允许深入了解免疫细胞 在组织定位的背景下的亚群。仪器将被放置在 统一流式细胞仪核心，匹兹堡大学学院的集中核心 医学。统一流核心目前服务于180多个PI和500个个人用户。为 主要用户包括13名Pis（10名NiH资助），另外5名Pis为次要用户。 总之，这些PI在NIH资助的直接成本中超过2000万美元， 不同的机构。一个主要的研究重点是在广泛的免疫学和相关免疫领域， 疾病，与姐妹学科，如移植，风湿病学，血管生物学， 和肿瘤学也很有代表性。虽然统一流式细胞术核心是良好的赋予， 传统的（虽然忙碌）分析仪和分选仪，ZellScanner芯片细胞仪带来了 我们的研究能力的全新广度。我们的初步和演示工作证明， 我们相信，这一工具将真正使我们的调查人员在他们的研究中开辟新的天地。 research.这台仪器的演示阶段确实在Pis中引起了极大的兴奋， 动手的用户。统一流式细胞仪的工作人员将对新机器进行很好的保养 Core拥有广泛的基础设施和文化来培训用户， 机器，并确保它是自支撑的。两名经验丰富的核心人员将负责 在ZellScanner单元上进行实验的概念和日常方面。我们将开始， 每天早上对机器进行认证，并排除任何问题，就像我们在 工作时间因此，它将对一个国家的生产力产生重大影响。 杰出且资金充足的PHS研究人员队列。