Defining Pathway-Specific Kinase Signaling Modules with Proteomics

摘要 蛋白的磷酸化是一种重要的调节修饰，它控制着许多细胞 流程。研究这种修饰的工具和方法的进展极大地扩展了 已知的磷酸化位点，但在我们的知识中仍有很大差距。我们将应用新的亲缘关系- 中心分析，研究规范的细胞信号通路，以确定特定途径的信号 模块。我们的目标是开发一种基于质谱学的表型分析，它可以识别 细胞信号的磷光信号。

Abstract Protein phosphorylation by kinases is an important regulatory modification that controls many cellular processes. Advances in tools and approaches to study this modification have greatly expanded the catalog of known phosphorylation sites but there remain substantial gaps in our knowledge. We will apply novel kinome- centric analyses to study canonical cell signaling pathways in order to define pathway-specific signaling modules. Our goal is to develop a mass spectrometry-based phenotyping assay that can identify phosphosignatures of cell signaling.