Defining Pathway-Specific Kinase Signaling Modules with Proteomics

用蛋白质组学定义通路特异性激酶信号传导模块

• 批准号: 9920727
• 负责人: Shao-En Ong
• 金额: $ 39.52万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-05-01 至 2023-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9920727
• 关键词: Address; Appearance; Benchmarking; Biological Assay; Cancer cell line; Catalogs; Cell Line; Cell model; Cell physiology; Cells; Complex; Data; Data Set; Development; Disease; Epidermal Growth Factor Receptor; Equipment and supply inventories; Genes; Genomics; Goals; Health; Human Genome; Impairment; Investigation; Kinetics; Knowledge; Learning; Ligands; Malignant Neoplasms; Malignant neoplasm of liver; Mass Spectrum Analysis; Methods; Modeling; Modification; Monitor; Morphologic artifacts; Noise; Pathway interactions; Phenotype; Phosphopeptides; Phosphorylation; Phosphorylation Site; Phosphotransferases; Post-Translational Protein Processing; Preparation; Proteomics; Publishing; Running; Sampling; Services; Signal Pathway; Signal Transduction; Signaling Molecule; Site; Specificity; Stimulus; System; The Cancer Genome Atlas; Time; Variant; analytical method; base; clinical diagnostics; druggable target; experimental study; instrumentation; insulin-like signaling; kinase inhibitor; novel; novel strategies; overexpression; phosphoproteomics; protein complex; public health relevance; specific biomarkers; tool; transcriptomics; trend

Abstract Protein phosphorylation by kinases is an important regulatory modification that controls many cellular processes. Advances in tools and approaches to study this modification have greatly expanded the catalog of known phosphorylation sites but there remain substantial gaps in our knowledge. We will apply novel kinome- centric analyses to study canonical cell signaling pathways in order to define pathway-specific signaling modules. Our goal is to develop a mass spectrometry-based phenotyping assay that can identify phosphosignatures of cell signaling.

摘要