Defining Pathway-Specific Kinase Signaling Modules with Proteomics

摘要 激酶引起的蛋白质磷酸化是一种重要的调节性修饰， 流程.研究这种修饰的工具和方法的进步极大地扩展了 已知的磷酸化位点，但在我们的知识中仍然存在很大的差距。我们将应用新的激酶组- 中心分析，以研究典型的细胞信号传导途径，以确定途径特异性信号传导 模块。我们的目标是开发一种基于质谱的表型分析方法， 细胞信号的磷酸化特征。

Abstract Protein phosphorylation by kinases is an important regulatory modification that controls many cellular processes. Advances in tools and approaches to study this modification have greatly expanded the catalog of known phosphorylation sites but there remain substantial gaps in our knowledge. We will apply novel kinome- centric analyses to study canonical cell signaling pathways in order to define pathway-specific signaling modules. Our goal is to develop a mass spectrometry-based phenotyping assay that can identify phosphosignatures of cell signaling.