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Development of a Next Generation Visual Performance Assessment System for Rodents

开发下一代啮齿动物视觉表现评估系统

基本信息

批准号：
9920144
负责人：
Dong Feng Chen
金额：
$ 69.17万
依托单位：
AFASCI, INC.
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-09-01 至 2022-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9920144
关键词：
Address Age related macular degeneration Algorithms Alzheimer&apos s Disease Animal Testing Animals Automation Behavior Behavior assessment Behavior monitoring Blindness Brain Brain Diseases Canada Cerebrum Comparative Study Computational algorithm Computer software Cone Consumption Contrast Sensitivity Development Devices Discrimination Disease model Drug Screening Engineering Evaluation Eye Eye Movements Eye diseases Future Gene Mutation Generations Germany Glaucoma Goals Grant Head Head Movements Histology Inherited Laboratories Legal patent Light Link Manuals Measurement Measures Mechanics Mediating Medical Methods Movement Mus Nerve Degeneration Neurodegenerative Disorders Optical Coherence Tomography Parkinson Disease Pathology Pharmaceutical Preparations Phase Phenotype Physiology Point Mutation Positioning Attribute Procedures Process Protocols documentation Rattus Reflex action Reproducibility Research Institute Research Personnel Resolution Retina Retinal Degeneration Retinitis Pigmentosa Rod Rodent Rodent Model Running Small Business Innovation Research Grant Stimulus System Tablets Technology Testing Time Transducin Translating Translations Traumatic Brain Injury Treatment Efficacy Validation Vertebrate Photoreceptors Vision Vision research Visual Visual Acuity Visual Pathway Disorder Visual impairment Wild Type Mouse aging brain base behavioral phenotyping behavioral response blind clinically relevant commercialization density design drug development drug discovery drug market effective therapy gaze gene function innovation luminance medical schools mouse model next generation non-compliance open source preclinical development preclinical evaluation prevent prototype response rho scale up tool treatment strategy validation studies vision science visual performance visual stimulus

项目摘要

7. PROJECT SUMMARY Title: Development of a Next Generation Visual Performance Assessment System for Rodents Visual behavior abnormalities not only link directly to the retinal pathology and visual pathway disorders, but also associate with many neurodegenerative brain conditions, particularly with Alzheimer’s disease, Parkinson’s disease, and traumatic brain injury. The optomotor reflex (OMR) consists of innate, reflexive head and/or body- movements during optomotor responses and has been a conventional method for measuring visual functions. It is emerging as a powerful tool for gene function phenotyping and drug screening in the vision research field, because it offers noninvasive and clinically-relevant assessments for contrast sensitivity and visual acuity. As preclinical development and evaluation of treatment strategies for neurodegenerative diseases are highly dependent on rodent models, especially mouse models, an OMR device for assessing rodent visual performance is in particular demand. However, existing OMR systems are either subjective (manually-scored OMR) or flawed as they rely on an arbitrary time window or an unmatched blind animal as a reference to determine vision limits. Also, the test procedure has not been optimized and is time consuming. The limitations of existing OMR technology and devices have largely hindered the evaluation of treatment efficacy and gene function behavior phenotyping in the field of vision science. In our completed Phase I project (R41 EY025913), we critically addressed the shortcomings of the existing OMR systems. Our effort has led to a new OMR system which utilizes patent-pending technology. The novelty of our system lies in the negative OMR indicator that underlies our unique algorithm. ; this algorithm enables researchers to distinguish between visually-impaired and noncompliant animals. Our optimized testing protocol uses a staircase reversal procedure, which allows for rapid, unbiased, and fully-automated visual assessment. The prototype of our system produces efficient and accurate real-time quantification of mouse visual performance with high sensitivity and reproducibility. The Specific Aims of this SBIR Phase II project are (1) to develop a “turnkey” OMR system, (2) to validate the system with multiple retina degenerative mouse models, and authenticate the test protocol as a guide to facilitate its application, and (3) to refine the system for rod- and cone-specific function discrimination. A platform for mouse and rat visual behavior assessment will greatly facilitate the drug discovery process aimed at preventing or slowing vision loss or restoring sight. Thereby, our product will accelerate the development of effective treatments for devastating blinding conditions including age-related macular degeneration and glaucoma. In the future, the system will be scaled up, and its application will be expanded to evaluate the aging brain and neurodegenerative disease models, such as Alzheimer’s disease, by measuring behavior responses toward various visual stimuli.

7. 项目总结