Flow Cytometry Shared Resource

流式细胞术共享资源：项目总结/摘要 流式细胞术辅助的定量细胞学是癌症研究人员的重要工具。客观 因此，流式细胞术共享资源（FCSR）的目的是提供具有成本效益和最先进的细胞 支持基础、转化和临床癌症的分析和细胞分选仪器和服务 弗吉尼亚联邦大学（VCU）梅西癌症中心（MCC）成员的研究活动， MCC的研究项目。FCSR还提供仪器和服务，以支持 表面等离子体共振（SPR），一种灵敏的无标记测定选择性的方法， 不同生物分子之间相互作用的亲和力。社会责任委员会支持这一使命， 主要仪器-2台先进的多激光细胞分选仪，2台多激光细胞分析仪，1台先进的成像仪 流式细胞仪和双通道SPR仪器。该资源由丹尼尔康拉德博士指导， 由2名全职员工提供支持。资源主任和/或工作人员通过提供以下服务来支持管理协委会的方案科学： 为实验设计和数据解释提供咨询服务;开展教育， 设备;操作员辅助细胞分选、细胞分析和SPR;方法开发;以及仪器 上维护所有FCSR服务都位于一个单一的中心位置，步行即可到达MCC。 FCSR是一个联合管理的（MCC和VCU）资源，设施的标准工作时间为上午8点至下午6点 周一到周五下午经过培训和认证的用户可以每天24小时，每天7天， 周设备时间和技术人员协助的活动得到跟踪和收费。提供的服务 由于这些服务的性质（通常涉及 活细胞）要求它们在当地可获得，并且如果来源于 商业供应商。社会研究基金会支持的工作继续对癌症产生积极影响 通过允许MCC成员以具有成本效益和效率的方式实现其研究目标， 方式事实上，在CY 2015中，FCSR支持了38个MCC成员的研究（占总数的58% 用户基础）。MCC成员的使用来自所有4个MCC研究项目的代表。

FLOW CYTOMETRY SHARED RESOURCE: PROJECT SUMMARY/ABSTRACT Quantitative cytology as facilitated by flow cytometry is an essential tool of cancer researchers. The objective of the Flow Cytometry Shared Resource (FCSR) is, therefore, to provide cost-effective and state-of-the-art cell analysis and cell sorting instrumentation and services in support of the basic, translational, and clinical cancer research activities of Virginia Commonwealth University (VCU) Massey Cancer Center (MCC) members and the MCC research programs. The FCSR additionally provides instrumentation and services in support of surface plasmon resonance (SPR), a sensitive method for the label-free determination of the selectivity and affinity of interactions between diverse biological molecules. The FCSR supports this mission by maintaining 6 major instruments—2 advanced multi-laser cell sorters, 2 multi-laser cell analyzers, an advanced imaging cytometer, and a dual-channel SPR instrument. The resource is directed by Daniel Conrad, PhD, and is supported by 2 FTEs. The resource director and/or staff support MCC programmatic science by providing: consultative services for experimental design and data interpretation; education to certify end-users in the use of the equipment; operator-assisted cell sorting, cell analysis, and SPR; method development; and instrument maintenance. All FCSR services are located in a single central location within easy walking distance of MCC. The FCSR is a jointly managed (MCC and VCU) resource, and standard hours for the facility are 8 AM until 6 PM, Monday through Friday. Trained and certified users have access to the facility 24 hours per day, 7 days per week. Equipment time and technician-assisted activities are tracked and charged back. The services provided by the FCSR add significant value for MCC-member research, as the nature of these services (often involving live cells) requires that they be available locally, and they would in any case be cost prohibitive if sourced from a commercial provider. The work supported by the FCSR continues to have a positive impact on cancer research by allowing MCC members to pursue their research objectives in a cost-effective and efficient manner. Indeed, in CY2015 FCSR supported the research of 38 MCC members (representing 58% of the total user base). MCC-member usage came from representatives of all 4 MCC research programs.