Flow Cytometry Laboratory Shared Resource

项目摘要/摘要 UWCCC于1988年成立了流式细胞术实验室(FLOW)，以提供最先进的 工具和专业知识，使我们的成员能够单独获取和分析复杂数据集 细胞和细胞群体，并分离确定的细胞群体以供进一步研究。 在当前的CCSG资金 循环，FLOW已经支持了137个独特的UWCCC成员的研究，这些成员代表了我们所有6个科学 程序。 我们已经获得了新的仪器，增加了我们的能力，建立了新的服务，并且 扩大我们针对UWCCC成员、他们的实习生和员工的教育计划。我们已经订婚了 UWCCC领导人和我们的科学咨询委员会制定一项战略计划，使我们能够 继续出色地完成我们的使命，满足UWCCC成员快速发展的研究需求。超过了 在下一个筹资周期中，我们将实现以下目标。具体目标1是为UWCCC提供 会员可以使用最先进的仪器进行单细胞分析和分类。流水屋， 操作和维护多参数分析细胞术所需的各种仪器， 成像细胞术和细胞分选。Flow已建立标准操作程序(SOP)， 确保所有仪器都经过适当的校准，并以高质量所需的方式进行操作 达到公认的实验严密性和重复性标准的数据集。具体目标2是 提供获取和分析多参数流式细胞仪数据所需的专业知识。细胞分析 和分拣技术正在快速发展，流量领导者监控这些进步并努力 确保UWCCC成员能够在他们的研究中充分利用最新技术。流量工作人员是 开发试剂板的专家，使成员能够利用我们的仪器收集多参数 光谱重叠最小的数据集。此外，Flow正在与我们的 生物统计学和癌症信息学共享资源，确保生成多参数数据集 在我们的工具上有适当的能力进行强有力的统计和信息学分析。具体目标3是 提供专家咨询和教育服务，使我们的用户能够充分利用我们的 具备单细胞分析和细胞分选能力。Flow工作人员与用户进行咨询，帮助他们 优化化验设计、数据采集、数据分析和数据呈现。我们在以下方面提供指导 仪器选型和操作。我们通知用户将适当的技术控制纳入他们的 实验。Flow为我们的用户提供定期安排的教学课程和实践培训，以提高 他们对流式细胞仪的了解，并提高他们在最低限度进行高质量研究的能力 可能的成本。Flow组织研讨会、研讨会和供应商演示，以扩展知识 我们的用户群。来自CCSG的支持将使我们能够继续提供高水平、经济高效的 为UWCCC成员提供服务，从而推进UWCCC的研究任务。

PROJECT SUMMARY / ABSTRACT The Flow Cytometry Laboratory (FLOW) was established by the UWCCC in 1988 to provide state-of-the-art instrumentation and expertise that enables our members to acquire and analyze complex data sets on single cells and cell populations and to isolate defined cell populations for further study. In the current CCSG funding cycle, FLOW has supported the research of 137 unique UWCCC members representing all 6 of our scientific programs. We have acquired new instrumentation, increased our capacity, established new services, and expanded our educational programs directed to UWCCC members, their trainees, and staff. We have engaged UWCCC leaders and our Scientific Advisory Committee to develop a strategic plan that will enable us to continue to excel in our mission and meet the rapidly evolving research needs of UWCCC members. Over the course of the next funding cycle we will achieve the following aims. Specific Aim 1 is to provide UWCCC members access to state-of-the-art instrumentation for single cell analytics and sorting. FLOW houses, operates, and maintains the broad assortment of instruments required for multi-parameter analytical cytometry, imaging cytometry, and cell sorting. FLOW has established Standard Operating Procedures (SOPs) that ensure all instruments are properly calibrated and operated in the manner required to yield high quality datasets that achieve accepted standards for experimental rigor and reproducibility. Specific Aim 2 is to provide expertise required for acquisition and analysis of multi-parameter flow cytometry data. Cell analytics and sorting technologies are advancing rapidly, and FLOW leaders monitor these advances and strive to ensure UWCCC members are able to fully exploit the newest technologies in their research. FLOW staff are experts in developing reagent panels that enable members to utilize our instruments to collect multi-parameter datasets with minimal spectral overlap. In addition, FLOW is developing a collaborative initiative with our Biostatistics and Cancer Informatics Shared Resources to ensure that the multi-parameter datasets generated on our instruments have appropriate power for robust statistical and informatics analyses. Specific Aim 3 is to provide expert consultation and education services that enable our users to take full advantage of our capabilities in single cell analytics and cell sorting. FLOW staff members consult with users to help them optimize assay design, data acquisition, data analysis, and data presentation. We provide guidance on instrument selection and operation. We inform users on incorporating appropriate technical controls into their experiments. FLOW offers regularly scheduled didactic courses and hands-on training to our users to advance their knowledge of flow cytometry and enhance their ability to perform high quality research at the lowest possible cost. FLOW organizes seminars, workshops, and vendor presentations that expand the knowledge base of our users. Support from the CCSG will enable us to continue to provide high level, cost-effective services to UWCCC members and thereby advance the UWCCC research mission.