Critical periods and complement regulation in diverse CNS cell types

项目总结/摘要 项目1 在Conte中心项目1中，我们将致力于了解中枢神经系统的细胞如何调节神经系统。 补体和其他免疫蛋白的表达，以及这种调节与 突触的功能和功能障碍。我们将通过临床实验、邮政实验来探讨这些问题。 尸体和生物样本我们将确定C4 A和C4 B的表达是如何被等位基因调控的。 变异、细胞类型和上游生物学（Aim 1）。我们将在单细胞分辨率下描述不同的CNS 细胞类型响应突触功能障碍，利用我们开发的Drop-seq技术分析 在数以万计的单个细胞中的转录应答（Aim 2）。最后，我们将认真评估 脑脊液作为CNS中神经免疫相互作用信息的潜在储存库（目的3）。 通过这项工作，我们希望更好地了解神经-免疫相互作用如何通过基因调控发挥作用。 表达，并且可以在临床环境中变得可见。

PROJECT SUMMARY / ABSTRACT Project 1 In Conte Center Project 1, we will work to understand how cells of the central nervous system regulate the expression of complement and other immune proteins, and the extent to which this regulation is coupled to function and dysfunction at synapses. We will pursue these questions through experiments on clinical, post mortem, and biological samples. We will determine how the expression of C4A and C4B is regulated by allelic variation, cell type, and upstream biology (Aim 1). We will describe at single-cell resolution how diverse CNS cell types respond to synaptic dysfunction, utilizing the Drop-seq technology we developed to analyzed transcriptional responses in tens of thousands of individual cells (Aim 2). Finally, we will carefully evaluate cerebrospinal fluid as a potential reservoir of information about neural-immune interactions in the CNS (Aim 3). Through this work, we hope to better understand how neural-immune interactions play out through gene expression and may become visible in a clinical context.