Core B - Instrumentation/Microscopy/Histopathology

核心B--项目摘要/摘要 仪器/显微镜/组织病理学核心为使用的设施和设备提供支持 由PPG成员共同发起。目标1是提供使用关键技术的优先访问和指导 仪器包括1)高速超速离心机；2)台风8610成像仪；3)ABI棱镜7900HT 实时PCR机；以及4)Beckman分光光度计DU7400。目标2是提供优先访问 指导并使用先进的PPG显微镜资源，包括Zeiss AxioImager套件、 蔡司Axiovert 200m三维反卷积活细胞成像显微镜，两台尼康钛-日食活细胞成像 去卷积显微镜和全自动尼康A1R共焦显微镜。目标3是提供PPG 拥有基本的共享组织病理学服务的成员，用于生成、处理和可视化福尔马林- 固定的、石蜡包埋的和冷冻的组织样本，用于肿瘤病毒相关癌症研究。加在一起， 这一核心(以前的“仪器”核心)在本供资期间得到了广泛使用， 现在扩展了它的能力，包括所需的额外显微镜和组织病理学能力 跨本P01的五个项目。

CORE B – PROJECT SUMMARY/ABSTRACT The Instrumentation / Microscopy / Histopathology Core provides support for facilities and equipment used communally by members of the PPG. Aim 1 is to provide priority access to and instruction in the use of critical instrumentation including 1) high-speed ultracentrifuges; 2) a Typhoon 8610 Imager; 3) an ABI Prism 7900HT real-time PCR machine; and 4) a Beckman Spectrophotometer DU7400. Aim 2 is to provide priority access to and instruction in the use of advanced PPG microscopy resources including a Zeiss Axioimager suite, a Zeiss Axiovert 200M 3-D deconvolution live cell imaging microscope, two Nikon Ti-Eclipse live cell imaging deconvolution microscopes, and a fully automated Nikon A1R confocal microscope. Aim 3 is to provide PPG members with essential shared histopathology services for generating, processing, and visualizing formalin- fixed, paraffin-embedded and frozen tissue samples for tumor virus-associated cancer studies. Combined, this Core (formerly the “Instrumentation” Core) has been extensively used over the current funding period, and is now expanded in its capacity to include additional microscopy and histopathology capabilities needed across the five projects of this P01.