Core B - Instrumentation/Microscopy/Histopathology

核心 B - 仪器/显微镜/组织病理学

• 批准号: 10414881
• 负责人: Nathan M Sherer
• 金额: $ 13.28万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-02-01 至 2024-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10414881
• 关键词: 3-Dimensional; Biochemistry; Cells; Disease; Epithelial Cells; Epstein-Barr Virus-Related Malignant Neoplasm; Equipment; Formalin; Freezing; Funding; Generations; Hepatitis B Virus; Histopathology; Human; Human Genetics; Human Herpesvirus 4; Human Herpesvirus 8; Human Papillomavirus; Instruction; Maintenance; Malignant Neoplasms; Microscope; Microscopy; Molecular Biology; Molecular Genetics; Names; Neoplasms; Oncogenic Viruses; Paraffin Embedding; Pathology; Preparation; Research; Resources; Services; Spectrophotometry; Speed; Supporting Cell; Time; Tissue Sample; Tissues; Training; Training Support; Tumor Virus Infections; Virus; Virus Replication; Work; commune; human disease; human tissue; imager; instrument; instrumentation; light microscopy; live cell imaging; member; microscopic imaging; mouse model; programs; success; temporal measurement; virus related cancer

CORE B – PROJECT SUMMARY/ABSTRACT The Instrumentation / Microscopy / Histopathology Core provides support for facilities and equipment used communally by members of the PPG. Aim 1 is to provide priority access to and instruction in the use of critical instrumentation including 1) high-speed ultracentrifuges; 2) a Typhoon 8610 Imager; 3) an ABI Prism 7900HT real-time PCR machine; and 4) a Beckman Spectrophotometer DU7400. Aim 2 is to provide priority access to and instruction in the use of advanced PPG microscopy resources including a Zeiss Axioimager suite, a Zeiss Axiovert 200M 3-D deconvolution live cell imaging microscope, two Nikon Ti-Eclipse live cell imaging deconvolution microscopes, and a fully automated Nikon A1R confocal microscope. Aim 3 is to provide PPG members with essential shared histopathology services for generating, processing, and visualizing formalin- fixed, paraffin-embedded and frozen tissue samples for tumor virus-associated cancer studies. Combined, this Core (formerly the “Instrumentation” Core) has been extensively used over the current funding period, and is now expanded in its capacity to include additional microscopy and histopathology capabilities needed across the five projects of this P01.

核心B -项目总结/摘要 仪器/显微镜/组织学核心为使用的设施和设备提供支持 由PPG的成员组成。目标1是提供优先获得和指导使用关键 仪器包括1）高速超浓缩机; 2）台风8610成像仪; 3）ABI Prism 7900 HT 实时PCR仪;和4）Beckman分光光度计DU 7400。目标2是提供优先访问 使用先进的PPG显微镜资源，包括蔡司Axioimager套件， Zeiss Axiovert 200 M三维去卷积活细胞成像显微镜，两台Nikon Ti-Eclipse活细胞成像 去卷积显微镜和全自动Nikon A1 R共聚焦显微镜。目标3：提供PPG 成员具有基本的共享组织病理学服务，用于生成，处理和可视化福尔马林- 用于肿瘤病毒相关癌症研究的固定、石蜡包埋和冷冻组织样本。结合起来， 该核心（以前的“仪器”核心）在当前的资助期内得到了广泛的使用， 现在，它的能力得到了扩展，包括所需的额外显微镜和组织病理学能力， 在本P01的五个项目中。