Enhancing the WGS based surveillance of Salmonella, Listeria, STEC and other foodborne related bacterial pathogens isolated from food and environment at the national and global level

在国家和全球层面加强对从食品和环境中分离出的沙门氏菌、李斯特菌、STEC 和其他食源性相关细菌病原体的基于全基因组测序的监测

• 批准号: 9933153
• 负责人: Paula Cray
• 金额: $ 16.47万
• 依托单位: NORTH CAROLINA STATE UNIVERSITY RALEIGH
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-05 至 2020-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9933153
• 关键词: Enhancing; WGS; based; surveillance; Salmonella

Salmonella, Listeria, STEC and other foodborne related bacterial pathogens isolated from retail meat, fresh produce and their environment have been implicated as the leading causes of bacterial foodborne illness in the US and around the world. It is important that we monitor the prevalence and trends of important foodborne bacterial strains in food products that can eventually pass to the consumers along the farm-to- fork chain resulting in significant public health impact. An important aim should be to expand the current surveillance network and monitor these important pathogens at the global scale to prevent future outbreaks. We propose to coordinate the GenomeTrakr WGS based surveillance program in partnership with the Pasteur Institute in Senegal to conduct surveillance of antimicrobial resistant foodborne bacterial pathogens using the `One Health” approach in humans, food products and the environment. Or extensive global network globally has allowed us to expand the GenomeTrakr program internationally. We strongly believe that the data generated through the proposed work will assist in monitoring Salmonella, Listeria, STEC and other foodborne related bacterial pathogens globally, where current surveillance systems are not robust enough to track them. The WGS data and metadata will significantly improve the power to resolve outbreaks by helping to quickly link clinical and retail product samples and assist in outbreak investigations. The PD (Dr. Thakur) laboratory, in partnership with the NC Department of Agriculture and Consumer Services (NCDACS), is a GenomeTrakr collaborating laboratory. Our proposal will significantly assist us in tracking emergence of potential new Salmonella, Listeria and STEC and other foodborne related bacterial strains and allow the public health agencies to take appropriate steps to safeguard human and animal health at the global level. The specific objectives of our proposal are: 1) Establish a robust surveillance system based on whole genome sequence (WGS) profile of 400 Salmonella, Listeria, STEC and other foodborne related bacterial pathogens isolated from food and environmental sources in the US and globally. 2) Compare the WGS profiles of Salmonella, Listeria, STEC and other foodborne related bacterial pathogens at the global scale to assist in outbreak investigations and track emerging strains of public health importance.

分离出沙门氏菌、李斯特菌、STEC 和其他食源性相关细菌病原体 零售肉类、新鲜农产品及其环境被认为是 美国和世界各地细菌性食源性疾病的主要原因。这是 重要的是，我们监测重要食源性细菌的流行率和趋势 食品中的菌株最终会从农场到消费者手中 分叉链对公共健康造成重大影响。一个重要的目标应该是 扩大当前的监测网络并监测这些重要的病原体 全球范围内预防未来的疫情爆发。我们建议协调 GenomeTrakr 与塞内加尔巴斯德研究所合作开展的基于 WGS 的监测计划 使用抗菌药物耐药性食源性细菌病原体进行监测 人类、食品和环境中的“同一个健康”方法。或广泛 全球网络使我们能够扩展 GenomeTrakr 计划 国际上。我们坚信，通过拟议工作生成的数据 将协助监测沙门氏菌、李斯特菌、STEC 和其他食源性相关菌 全球细菌病原体，目前的监测系统不够健全 来追踪他们。 WGS 数据和元数据将显着提高 通过帮助快速链接临床和零售产品样本来解决疫情并协助 在疫情调查中。 PD（Thakur 博士）实验室与 NC 合作 农业和消费者服务部 (NCDACS) 是 GenomeTrakr 合作实验室。我们的提案将极大地帮助我们追踪出现情况 潜在的新沙门氏菌、李斯特菌和 STEC 以及其他食源性相关细菌 压力并允许公共卫生机构采取适当措施来保障 全球范围内的人类和动物健康。 我们提案的具体目标是： 1）建立基于全基因组序列（WGS）的强大监测系统 400 种沙门氏菌、李斯特菌、STEC 和其他食源性相关细菌的概况 从美国和全球的食品和环境来源中分离出的病原体。 2) 比较沙门氏菌、李斯特菌、STEC 和其他食源性细菌的 WGS 图谱 全球范围内的相关细菌病原体，以协助疫情调查和 追踪对公共卫生具有重要意义的新出现的菌株。