Monitoring antimicrobial resistance (AMR) in Salmonella, Campylobacter, Escherichia coli and Enterococcus spp. isolated from retail meats in North Carolina

监测沙门氏菌、弯曲杆菌、大肠杆菌和肠球菌的抗菌素耐药性 (AMR)。

• 批准号: 9550824
• 负责人: Paula Cray
• 金额: $ 13万
• 依托单位: NORTH CAROLINA STATE UNIVERSITY RALEIGH
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9550824
• 关键词: Monitoring; antimicrobial; resistance; AMR; Salmonella

Food animals, including retail pork, chicken, ground turkey and ground beef have been implicated as sources of Salmonella and Campylobacter and are the leading causes of bacterial foodborne illness. Indicator pathogens, including Escherichia coli and Enterococcus spp., are also frequently isolated from these meat products. The situation is further complicated when the strains are also resistant to different antimicrobials. It is, therefore, important that we monitor the prevalence and trends of antimicrobial resistant (AMR) foodborne bacterial strains in the retail meat products that can eventually pass to the consumers along the farm-to-fork chain resulting in significant public health impact. The National Antimicrobial Resistance Monitoring System (NARMS) plays an important role in surveillance of AMR bacterial pathogens in humans, food animals and retail meats. Though North Carolina is an important food producing state in the US and ranks among the top states in pork and poultry production, it is currently not a part of the NARMS program. Our major objective, therefore, is to enhance the surveillance of AMR pathogenic and indicator bacterial strains in retail meats in North Carolina and generate critical data to contribute to the NARMS mission. We propose to conduct this study in collaboration with the FDA GenomeTrakr program and strong support from the NC Department of Agriculture and Consumer Services (NCDACS). The PI (Dr. Thakur) laboratory is a GenomeTrakr laboratory, which will provide the opportunity to generate the whole genome sequences (WGS) of the bacterial strains thereby significantly assisting us in tracking the emergence of potential new AMR bacterial and allowing the public health agencies to take appropriate steps. The specific objectives of our proposal are: 1) Monitor antimicrobial resistance (AMR) trends in Salmonella, Campylobacter, E. coli and Enterococcus spp. from retail meat samples collected in North Carolina region. 2) Generate and compare the WGS profiles of Salmonella, Campylobacter, E. coli and Enterococcus spp. isolated from retail meat in North Carolina to detect the emergence of new AMR strains. The long-term objective of our proposal is to protect and promote public health by enhancing and strengthening the surveillance of AMR Salmonella, Campylobacter, E. coli and Enterococcus spp. in retail food specimens the US.

食用动物，包括零售猪肉、鸡肉、火鸡肉和牛肉， 被认为是沙门氏菌和弯曲杆菌的来源，是导致 细菌性食源性疾病指示病原体，包括大肠杆菌和 肠球菌属，也经常从这些肉类产品中分离出来。局势 当菌株对不同的抗菌剂也有抗性时，是的， 因此，重要的是，我们监测抗生素耐药的流行和趋势， (AMR)零售肉制品中的食源性细菌菌株最终会传播到 消费者沿着从农场到餐桌的链条，造成重大的公共卫生影响。 国家抗菌药物耐药性监测系统（NARMS）在 在监测人类、食用动物和零售业中AMR细菌病原体方面的作用 肉。虽然北卡罗来纳州是美国重要的粮食生产州， 在猪肉和家禽生产最多的州中，它目前不是美国的一部分。 NARMS程序。因此，我们的主要目标是加强对AMR的监测 北卡罗来纳州零售肉类中的致病性和指示性细菌菌株， 为NARMS使命做出贡献的关键数据。我们建议进行这项研究， 与FDA GenomeTrakr计划的合作以及NC的大力支持 农业和消费者服务部（NCDACS）。主要研究者（Thakur博士） 实验室是一个GenomeTrakr实验室，它将提供机会， 细菌菌株的全基因组序列（WGS）由此显著地 帮助我们跟踪潜在的新AMR细菌的出现， 公共卫生机构采取适当措施。 我们建议的具体目标是： 1)监测沙门氏菌、弯曲杆菌、大肠杆菌的抗菌素耐药性（AMR）趋势。杆菌 和肠球菌属（Enterococcus spp.）从北卡罗来纳州的零售肉类样本中提取的。 2)比较沙门氏菌、弯曲杆菌、大肠杆菌的WGS图谱。杆菌和 肠球菌属从北卡罗来纳州的零售肉类中分离出来， 新的AMR菌株。 我们建议的长远目标，是透过以下措施，保障和促进公众健康 加强对耐甲氧西林沙门氏菌、弯曲杆菌、大肠杆菌的监测。 大肠杆菌和肠球菌属（Enterococcus spp.）在美国零售食品标本。