Enhancing the whole genome sequence(WGS)based surveillance of Salmonella, Listeria and Shiga Toxin producing Escherichia coli(STEC) isolated from food and environment at the national and global level

在国家和全球层面加强对从食品和环境中分离出的沙门氏菌、李斯特菌和志贺毒素大肠杆菌 (STEC) 的全基因组序列 (WGS) 监测

• 批准号: 9479495
• 负责人: Paula Cray
• 金额: $ 16.5万
• 依托单位: NORTH CAROLINA STATE UNIVERSITY RALEIGH
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-05 至 2019-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9479495
• 关键词: Enhancing; whole; genome; sequence; WGS

Salmonella, Listeria and STEC isolated from retail meat, fresh produce and their environment have been implicated as the leading causes of bacterial foodborne illness in the US and around the world. It is important that we monitor the prevalence and trends of important foodborne bacterial strains in food products that can eventually pass to the consumers along the farm-to-fork chain resulting in significant public health impact. It is, therefore, important to expand the current surveillance network and monitor these important pathogens at the global scale to prevent future outbreaks. We propose to do this in collaboration with the WHO-AGISAR (Advisory Committee on Integrated Surveillance of Antimicrobial Resistance) which funds countries to conduct surveillance of antimicrobial resistant foodborne bacterial pathogens in humans, food products and the environment. We strongly believe that the data generated through the proposed work will assist in monitoring Salmonella, Listeria and STEC from at the international level where current surveillance systems are not robust enough to track them. The WGS data and metadata will significantly improve the power to resolve outbreaks by helping to quickly link clinical and retail product samples and assist in outbreak investigations. The PD (Dr. Thakur) laboratory, in partnership with the NC Department of Agriculture and Consumer Services (NCDACS), is a GenomeTrakr collaborating laboratory. Our proposal will significantly assist us in tracking emergence of potential new Salmonella, Listeria and STEC strains and allow the public health agencies to take appropriate steps to safeguard human and animal health at the global level. The specific objectives of our proposal are: 1) Establish a robust surveillance system based on whole genome sequence (WGS) profile of 400 Salmonella, Listeria and STEC isolated from food and environmental sources within and outside the US. 2) Compare the WGS profiles of Salmonella, Listeria and STEC at the global scale to assist in outbreak investigations and track emerging strains of public health importance. The long-term objective of our proposal is to protect and promote public health by strengthening the surveillance of Salmonella, Listeria and STEC within and outside the US by generating critical WGS data and contributing to the GenomeTrakr mission.

从零售肉类、新鲜农产品及其制品中分离出沙门氏菌、李斯特菌和 STEC 环境被认为是细菌性食源性疾病的主要原因 在美国和世界各地。重要的是我们要监测患病率并 食品中最终可能通过的重要食源性细菌菌株的趋势 从农场到餐桌的整个链条上的消费者，从而产生重大的公共健康 影响。因此，扩大现有的监控网络并进行监控非常重要。 在全球范围内识别这些重要的病原体，以防止未来的疫情爆发。我们建议 与 WHO-AGISAR（综合咨询委员会）合作开展这项工作 抗菌素耐药性监测）资助各国进行监测 人类、食品和食品中对抗菌药物耐药的食源性细菌病原体 环境。我们坚信，通过提议生成的数据 工作将协助在国际范围内监测沙门氏菌、李斯特菌和 STEC 目前的监控系统还不够强大，无法跟踪它们。这 WGS 数据和元数据将显着提高解决疫情爆发的能力 帮助快速链接临床和零售产品样本，协助疫情爆发 调查。 PD（Thakur 博士）实验室与 NC 部门合作 农业和消费者服务部 (NCDACS) 是 GenomeTrakr 合作 实验室。我们的建议将极大地帮助我们追踪潜在的出现 新的沙门氏菌、李斯特菌和 STEC 菌株，并允许公共卫生机构采取 在全球范围内采取适当措施保障人类和动物健康。 我们提案的具体目标是： 1）建立基于全基因组序列（WGS）的强大监测系统 从食品和环境中分离出的 400 种沙门氏菌、李斯特菌和 STEC 的概况 美国境内外的来源。 2) 比较全球范围内沙门氏菌、李斯特菌和 STEC 的 WGS 概况 协助疫情调查并追踪对公共卫生具有重要意义的新出现的菌株。 我们提案的长期目标是通过以下方式保护和促进公众健康： 加强境内外沙门氏菌、李斯特菌和STEC的监测 美国通过生成关键的 WGS 数据并为 GenomeTrakr 任务做出贡献。