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Mechanisms underlying spatial interaction in the oral microbiota

口腔微生物群空间相互作用的潜在机制

基本信息

批准号：
9974507
负责人：
Matthew Ramsey
金额：
$ 40.66万
依托单位：
UNIVERSITY OF RHODE ISLAND
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-07-08 至 2024-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9974507
关键词：
Adherence Adhesions Aerobic Anaerobic Bacteria Bacteria Bacterial Adhesins Binding Biochemical Biological Assay Coculture Techniques Communities Corynebacterium Data Development Disease Erinaceidae Feeds Genes Goals Growth Haemophilus parainfluenzae Health Hemophilus Human Hydrogen Peroxide Image Impairment In Vitro Individual Integration Host Factors Intervention Knowledge Libraries Measurement Mediating Metabolic Methods Microbial Biofilms Microscopy Molecular Mouth Diseases Mutagenesis Opportunistic Infections Organism Oxidative Stress Pattern Probiotics Production Reproducibility Research Salivary Salivary Proteins Sampling Scanning Shapes Streptococcus Streptococcus cristatus Streptococcus mitis Structure Suggestion Surface Technology Testing Transcript base biological adaptation to stress dental biofilm design experimental study feeding gene product imaging probe in vivo innovation microbiome mutant oral microbial community oral plaque oral streptococci preservation prevent prophylactic receptor transcriptome transcriptome sequencing

项目摘要

ABSTRACT Human oral plaque is a polymicrobial community whose composition varies during health and disease. Recent advances in technology have yielded new information on the identity and abundance of constituent species during these conditions yet do not allow predictions of direct interactions between specific organisms. The biogeography of reproducible structures within supragingival plaque has been characterized through advanced microscopy methods suggesting key organisms that may help arrange plaque structure. Species-species co-proximity within these ordered structures is suggestive of metabolite mediated interactions between them. Our global hypothesis is that adjacent species in healthy plaque biofilms have specific metabolic and physical interactions that shape both the physical and compositional structure of this community. To identify mechanistic interactions between adjacent species, we must determine which species exist together in vivo. Preliminary data indicates that the highly abundant Corynebacterium matruchotii and Haemophilus parainfluenzae bacterial species exist directly adjacent to several Streptococcus spp. suggesting they must be able to tolerate pH and oxidative stress produced by oral streptococci. Direct imaging further suggests that some species bind directly to each other or mutually to a host intermediate such as salivary protein. The physical means of attachment for C. matruchotii and H. parainfluenzae to Streptococcus spp. are unknown. This proposal will determine which Streptococcus species interact with C. matruchotii and H. parainfluenzae via microscopy (Aim 1). It will also determine if C. matruchotii and H. parainfluenzae participate in cross- feeding interactions with streptococcal produced metabolites and will identify mechanisms that either bacterium uses to tolerate pH and oxidative stress through transcriptome analyses, mutant library assays, and quantitative metabolite measurements (Aim 2). Lastly, our proposal will determine physical factors produced by bacteria that are responsible for co-adhesion between different species. These will be identified by direct and random mutagenesis of C. matruchotii and H. parainfluenzae and tested in combination with known interacting Streptococcus species. The goal of this proposal is to identify interacting species in healthy supragingival plaque and characterize mechanistic interactions between them, revealing how they may contribute to plaque structure and composition. Our rationale is that we will identify mechanisms that promote interactions between highly abundant organisms in healthy plaque and identify candidate species and their associated interactions for use in probiotic or prophylactic interventions to manage oral plaque communities to prevent opportunistic infection.

摘要