Mechanisms of gamma delta intraepithelial lymphocyte-mediated host defense

γδ上皮内淋巴细胞介导的宿主防御机制

• 批准号: 9976324
• 负责人: Madeleine Hu
• 金额: $ 4.21万
• 依托单位: RBHS-NEW JERSEY MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-01 至 2021-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9976324
• 关键词: Academic Medical Centers; Acute; Advisory Committees; Anti-Bacterial Agents; Antibacterial Response; Apoptotic; Autoimmunity; Automobile Driving; Bacteria; Bacterial Translocation; Basic Science; Behavior; Cells; Clinical; Data; Development; Enteral; Enterocytes; Epithelial; Epithelial Cells; Epithelium; Event; Exhibits; Extracellular Space; Failure; Focal Infection; Foundations; Future; Goals; Health; Home environment; Host Defense; Immune; Immune system; In Vitro; Infection; Inflammation; Inflammatory Bowel Diseases; Inflammatory Response; Institution; Intestinal Mucosa; Intestines; Knockout Mice; Knowledge; Laboratories; Lateral; Lead; Lipopolysaccharides; Lymphocyte; Lymphocyte Function; Maintenance; Mediating; Mentors; Microbe; Migration Assay; Mission; Molecular; Mucous Membrane; Mus; National Institute of Diabetes and Digestive and Kidney Diseases; Nuclear Translocation; Pathologic; Pattern; Pattern Recognition; Peritoneal; Phenotype; Physicians; Predisposition; Process; Production; Public Health; Receptor Signaling; Regulation; Relapse; Reporter; Research; Research Training; Role; Salmonella; Salmonella typhimurium; Scientist; Signal Transduction; Skin; Systemic infection; T-Cell Receptor; Testing; Therapeutic; Toll-like receptors; Training; Translational Research; United States National Institutes of Health; Wild Type Mouse; Work; adaptive immune response; antimicrobial peptide; base; career; cell motility; collaborative environment; commensal bacteria; cytokine; human disease; improved; in vivo; insight; interest; intestinal epithelium; intraepithelial; intravital microscopy; microbial; migration; monolayer; novel; pathogen; pathogenic bacteria; prevent; programs; response; skills; translational medicine; γδ T cells

PROJECT SUMMARY/ABSTRACT. The intestinal epithelium is the first line of defense against the trillions of bacteria in the intestinal lumen. Failure to prevent or limit bacterial invasion can lead to infection and may trigger a harmful inflammatory response, as seen in inflammatory bowel disease. Intraepithelial lymphocytes (IEL) expressing the γδ T cell receptor rapidly respond to bacterial translocation through modulation of their migratory behavior and release of soluble host defense factors, including cytokines and antimicrobial peptides (AMP). Though γδ IELs are an essential part of host antibacterial response, many of the processes surrounding γδ IEL-mediated host defense remain unclear. The objective of this application is to elucidate the mechanisms through which γδ IELs contribute to host antibacterial defense. Preliminary data suggests that γδ IEL surveillance behavior and effector function may be partially mediated by cell-autonomous recognition of microbe-associated molecular patterns (MAMP) through Toll-like receptor/MyD88 signaling. Moreover, a newly-identified requirement for γδ IELs in lipopolysaccharide (LPS)-induced epithelial cell shedding may represent a novel form of γδ IEL- mediated host antibacterial defense, as extrusion of enterocytes infected with intracellular bacteria limits pathogen proliferation within the epithelium. These observations have led to the central hypothesis that γδ IELs contribute to host defense against bacterial invasion by activating migratory and effector responses following cell-autonomous MAMP recognition and by promoting the shedding of infected enterocytes. The aims of this application are to 1) determine the contributions of γδ IEL MyD88 signaling to host defense against acute bacterial invasion and 2) determine the mechanisms through which γδ IELs regulate cell shedding in response to acute bacterial challenge. The role of γδ IEL MyD88 in mediating MAMP-induced changes in γδ IEL migration and cytokine/AMP secretion will be assessed using inducible, γδ T-cell-specific MyD88 knockout mice and a combination of in vitro and in vivo approaches. Further, the contribution of γδ IEL MyD88 to antibacterial defense will be determined using Salmonella Typhimurium infections. Next, the mechanisms by which γδ T cells promote LPS-induced epithelial cell shedding will be interrogated by quantifying cell shedding in mice exhibiting altered γδ IEL migratory phenotypes or following γδ TCR inhibition. The requirement for γδ IELs in shedding of Salmonella-infected enterocytes will be assessed at early infection timepoints. Completion of these aims will provide novel insight into the mechanisms driving γδ IEL-mediated antibacterial defense. The results of these studies will contribute towards the long-term goal of determining the therapeutic potential of targeting γδ IEL effector functions as a means to prevent excessive inflammation by promoting rapid clearance of infection. The proposed research and training plan, along with my mentors, advisory committee, and the interdisciplinary environment at my home institution, will help me achieve my long- term career goal of becoming an effective physician-scientist involved in independent translational research.

项目总结/抽象。