Analyzing pathogenic Vibrio parahaemolyticus-oyster interactions to prevent human disease

分析致病性副溶血性弧菌与牡蛎的相互作用以预防人类疾病

• 批准号: 9979077
• 负责人: Ann M Stevens
• 金额: $ 8.01万
• 依托单位: VIRGINIA POLYTECHNIC INST AND ST UNIV
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-03-01 至 2022-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9979077
• 关键词: Acute; Adolescent; Animal Model; Animals; Aquaculture; Bacteria; Bacterial Proteins; Bacteriology; Beds; Bioinformatics; Biological Models; Biology; Cells; Centers for Disease Control and Prevention (U.S.); Clinical; Consumption; Detection; Development; Disease; Eating; Ecology; Environmental Risk Factor; Epithelial; Epithelium; Excision; Food; Food Safety; Foundations; Future; Gastroenteritis; Genes; Genetic; Goals; Harvest; Health; Human; Immunocompromised Host; Individual; Infection; Intervention; Laboratories; Lead; Libraries; Life; Location; Methods; Mission; Modeling; Molecular; Morbidity - disease rate; Oysters; Palliative Care; Pathogenicity; Pathology; Phase; Play; Prevention strategy; Production; Public Health; Regulation; Research; Role; Route; Scientist; Seafood; Shellfish; Site; Source; System; Systemic infection; Testing; Tissues; United States; United States National Institutes of Health; Vibrio; Vibrio parahaemolyticus; Virulence; Water; Work; animal breeding; animal tissue; base; disorder prevention; economic impact; emerging human pathogen; food surveillance; foodborne illness; gastrointestinal; genetic approach; genome-wide; human disease; human morbidity; human mortality; improved; in vivo; infection rate; insight; method development; microbiome; mortality; mutant; novel; novel strategies; open wound; pandemic disease; pathogen; pathogenic bacteria; prevent; risk minimization; therapy development; transmission process; transposon sequencing; undercooked

Project Summary/Abstract The bacterium Vibrio parahaemolyticus (VP) is a leading cause of seafood-borne gastroenteritis on a pandemic scale. In the United States, it is a CDC-reportable emerging human pathogen with increasing annual morbidity and mortality rates. Diseases caused by pathogenic VP strains have important implications for global food safety and sustainability in relation to human health. Most commonly, human disease prevention has focused on the detection and reduction of VP in food sources. Despite this, human VP infection rates are still rising. Little is known about how VP interacts with its marine animal hosts. A better understanding of this relationship has the potential to lead to the development of methods to either prevent bacterial-animal host association and/or enhance bacterial removal from the host prior to human consumption, thereby mitigating the occurrence of human disease. We propose to use oysters, a natural host of VP and a common route of transmission to humans, as an animal model system to understand when and how VP interacts with them. It is hypothesized that A) VP will localize to specific tissues in the oyster and B) specific genes will be critical for the ability of VP to colonize oysters. The proposed work is strengthened by our collaborative research team, which is comprised of four scientists with expertise in molecular bacteriology, shellfish aquaculture, animal pathology and bioinformatics methods. The novel information gained from this research will lead to a more reliable laboratory-based oyster model system that will lay the foundation for future work to establish the roles environmental factors, including the resident microbiome, host genetics and specific bacterial gene products play in VP-oyster interactions. The refined oyster model will be applicable not only for VP but for other pathogenic Vibrio species, leading to improved methods to prevent human food-borne disease acquisition.

项目总结/摘要 副溶血性弧菌（VP）是导致海产品传播胃肠炎的主要原因， 大流行规模。在美国，它是CDC报告的新兴人类病原体， 发病率和死亡率。致病性VP菌株引起的疾病对全球 与人类健康有关的食品安全和可持续性。最常见的是，人类疾病预防 重点是检测和减少食物来源中的VP。尽管如此，人类VP感染率仍然是 升中国关于VP如何与其海洋动物宿主相互作用，人们知之甚少。更好地理解本 这种关系有可能导致开发出预防细菌-动物宿主的方法 结合和/或增强在人类食用之前从宿主去除细菌，从而减轻 人类疾病的发生。我们建议使用牡蛎，这是VP的天然宿主，也是VP的常见途径。 传播给人类，作为一个动物模型系统，以了解何时以及如何VP与他们相互作用。是 假设A）VP将定位于牡蛎的特定组织，B）特定基因对 VP在牡蛎上定居的能力。我们的合作研究小组加强了拟议的工作， 是由四名科学家组成，他们在分子细菌学、贝类水产养殖、动物病理学 和生物信息学方法。从这项研究中获得的新信息将导致一个更可靠的 基于实验室的牡蛎模型系统，为未来建立角色的工作奠定基础 环境因素，包括常驻微生物组、宿主遗传学和特定细菌基因产物 参与VP与牡蛎的互动。改进的牡蛎模型不仅适用于VP，而且适用于其他 致病性弧菌物种，导致改进的方法，以防止人类食源性疾病的收购。