Evaluating the relationship of CD101 and UBE2V1 to genital mucosal inflammation

评估CD101和UBE2V1与生殖器粘膜炎症的关系

• 批准号: 9979746
• 负责人: Jairam Rao Lingappa
• 金额: $ 77.94万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-14 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9979746
• 关键词: 5' Untranslated Regions; AIDS prevention; Adherence; African; Anti-Retroviral Agents; Antigens; Bacterial Vaginosis; Biological; Biopsy; CCL7 gene; CXCL9 gene; Cells; Couples; Data; Detection; Drug Targeting; Drug resistance; Effector Cell; Enrollment; Female; Functional disorder; Future; Gene Frequency; Genes; Genetic Determinism; Genetic Transcription; Genital system; Genomics; HIV-1; Heterosexuals; Host resistance; Human Herpesvirus 2; Immune; Immune response; Immunosuppression; In Vitro; Individual; Infection; Inflammation; Inflammatory; Inflammatory Response; Interleukin-7; Intervention; Link; Minor; Modeling; Molecular; Mucositis; Mucous Membrane; Pathway interactions; Pattern; Pharmaceutical Preparations; Plasma; Prevention; Preventive Intervention; Public Health; Regulation; Regulatory T-Lymphocyte; Reporting; Risk; Risk Factors; Role; Sampling; Sexually Transmitted Diseases; TNF gene; Testing; Tissues; Untranslated Regions; Vaccines; Variant; Viral; Visit; Woman; chemokine; co-infection; cost; cytokine; epidemiology study; experience; female sex worker; genetic association; genetic variant; genital herpes; genital infection; genome sequencing; hazard; immune activation; infection risk; inflammatory milieu; men; novel; novel strategies; pathogen; pre-exposure prophylaxis; prevent; recruit; reproductive tract; risk minimization; whole genome

ABSTRACT Global HIV-1/AIDS prevention efforts have been greatly facilitated by the demonstration that anti-retroviral drugs (ARVs) for pre-exposure prophylaxis (PrEP) and treatment as prevention can be effective in preventing the spread of HIV-1. However, the challenges of achieving high adherence, the cost of implementation, and potential for future changes in ARV drug resistance patterns underscore the continuing need for new approaches to HIV- 1 prevention. In particular, novel interventions that target host immune responses may be less likely to elicit viral escape, and may enhance host responses to future HIV-1 prevention vaccines. Genital tract inflammation is known to be associated with altered risk of HIV-1 acquisition. Studies have reported that inflammatory responses to sexually transmitted infections (STI), the presence of genital tract inflammatory cytokines (e.g., IL-7, TNF-α, and IL12p70), chemokines (e.g., CCL7, and CXCL9), and cellular immune activation are associated with increased risk of HIV-1 infection. One explanation for these observations is that these inflammatory environments result in the accumulation of activated immune effector cells, which increase available targets for HIV-1 infection. However, studies of Kenyan female sex workers have found that natural host resistance to HIV-1 infection is associated with lower levels of immune activation (e.g., “immune quiescence”). This reduced immune activation did not reflect a state of broad immunosuppression insofar as these women had otherwise normal host responses to exogenous antigens. This suggests that intrinsic host pathways may regulate genital tract inflammatory responses, and these regulatory mechanisms may alter the risk of HIV-1 infection. However, studies also report that, in some settings, immune activation may be associated with reduced HIV-1 acquisition, raising the caution that use of interventions to broadly induce nonspecific immunosuppression could have unintended effects, such as eliminating effective barriers to HIV-1 or to defenses against other co-infections. The public health challenge is to identify genital tract inflammatory pathway(s) that can mitigate risk of HIV-1 acquisition without increasing risk of other infections. We recently documented the presence of variants in two genes, CD101 and UBE2V1, which are strongly associated with altered HIV-1 infection risk. Both of these genes have been previously reported to have roles in regulating host inflammatory responses. Given these genetic associations with HIV-1 acquisition risk, and a priori evidence that these genes may modify host inflammatory responses, we hypothesize that these genes may modulate HIV-1 acquisition risk through altering underlying host inflammatory responses in the genital tract. Here we propose molecular and epidemiologic studies to evaluate the association of these gene variants with inflammation of the female genital tract.

摘要 抗逆转录病毒药物的证明极大地促进了全球艾滋病毒/艾滋病预防工作 暴露前预防(PrEP)和作为预防的治疗可以有效地预防 HIV-1的传播。然而，实现高度遵守的挑战、实施成本和潜力 对于未来抗逆转录病毒耐药性模式的变化，强调了继续需要新的方法来应对艾滋病毒- 1预防。特别是，针对宿主免疫反应的新型干预措施可能不太可能引发病毒 逃逸，并可能增强宿主对未来艾滋病毒-1预防疫苗的反应。 已知生殖道炎症与HIV-1感染风险的改变有关。研究表明， 报道称，炎症反应对性传播感染(STI)、生殖道的存在 炎性细胞因子(例如IL-7、肿瘤坏死因子-α和IL 12p70)、趋化因子(例如CCL7和CXCL9)和细胞 免疫激活与HIV-1感染风险增加有关。对这些观察结果的一种解释 这些炎性环境会导致激活的免疫效应细胞的积累，这 增加艾滋病毒-1感染的可用目标。然而，对肯尼亚女性性工作者的研究发现 自然宿主对HIV-1感染的抵抗力与较低水平的免疫激活有关(例如，免疫 静默“)。这种免疫活性的降低并不反映在以下情况下的广泛免疫抑制状态 这些妇女在其他方面对外源抗原有正常的宿主反应。这表明内在宿主 多种途径可能调节生殖道炎症反应，而这些调节机制可能 改变感染HIV-1病毒的风险。然而，研究也报告说，在某些情况下，免疫激活可能 与减少HIV-1感染有关，提高了使用干预措施广泛诱导 非特异性免疫抑制可能会产生意想不到的影响，例如消除艾滋病毒-1的有效屏障 或者用来防御其他混合感染。公共卫生面临的挑战是识别生殖道 炎症途径(S)，可在不增加其他感染风险的情况下降低感染艾滋病毒-1的风险 感染。 我们最近记录了两个基因CD101和UBE2V1中存在变异，这两个基因具有很强的 与改变的HIV-1感染风险有关。这两个基因之前都被报道在 调节宿主炎症反应。鉴于这些基因与HIV-1感染风险的关联，以及先验 这些基因可能改变宿主炎症反应的证据，我们假设这些基因可能 通过改变生殖道内潜在的宿主炎症反应来调节HIV-1感染风险。这里 我们建议进行分子和流行病学研究，以评估这些基因变异与 女性生殖道发炎。