Application and extension of dye-based food consumption methodology in Drosophila

基于染料的食物消耗方法在果蝇中的应用和推广

• 批准号: 9980753
• 负责人: MICHAEL S. GROTEWIEL
• 金额: $ 3.88万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-01 至 2022-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9980753
• 关键词: Address; Aging; Behavior; Complex; Consumption; Development; Diet; Disease; Disease Marker; Disease Progression; Drosophila genus; Drosophila melanogaster; Dyes; Eating; Environmental Risk Factor; Equipment; Ethanol; Excretory function; Feeding Methods; Food; Foundations; Genes; Genetic; Goals; Health; Housing; Human; Incidence; Intake; Laboratories; Laboratory Organism; Lead; Longevity; Measures; Metabolism; Methodology; Methods; Minor; Modeling; Modification; Molecular; Musca domestica; Non-Insulin-Dependent Diabetes Mellitus; Nutrient; Obesity; Obesity Epidemic; Obesity associated disease; Organism; Partner in relationship; Physiological Adaptation; Physiology; Prevention; Process; Reagent; Reporting; Resources; Role; Sedation procedure; Solid; Starvation; Time; Tracer; Translating; base; dietary restriction; egg; experimental study; fly; food consumption; improved; response; sex determination

Project Summary/Abstract: Over the past 100+ years, studies in Drosophila melanogaster (Drosophila or flies) have contributed enormously to our understanding of the genetic basis for sex-determination, development, behavior, aging, disease and many other normal as well as pathophysiological processes. More recently, the fly model has also been used to explore the contribution of environmental factors in health and disease. Key among the environmental factors being investigated is diet. Altering the diet of flies has profound effects on their physiology, progression of disease markers and aging. For example, dietary restriction in flies (via reduced concentrations of nutrients) extends lifespan as found in other species. Changes in the fly diet also greatly impact egg-laying (an indicator of resource utilization) and can lead to the development of a type II diabetes-like state. Furthermore, our preliminary studies indicate that diet has a pronounced effect on ethanol sedation behavior in flies. Given the effects of diet in flies, the ongoing global obesity epidemic and the increasing incidence of obesity-related diseases, studies in flies hold tremendous promise for continuing to uncover key mechanisms underlying diet-related phenomena that could ultimately translate into improved prevention and treatment of a multitude of diet-related diseases in humans. We have consequently begun developing dye-based methods (Con-Ex) for measuring food consumption in flies. The overarching goals of the studies in this application are to (i) use our previously established dye-based Con-Ex feeding method with the dye Blue-1 as a food tracer to assess intake of diets that alter longevity—and importantly do so across the lifespans of flies and (ii) extend the utility of the method by identifying and validating dyes in addition to Blue-1 for use in Con-Ex studies. The proposed studies will address an important question related to the effects of diet on longevity and will lay the foundation for more sophisticated dietary consumption analyses in flies.

项目摘要/摘要：在过去的100多年里，对黑腹果蝇(Drophila Blackogaster)的研究 苍蝇)对我们理解性别决定的遗传基础做出了巨大贡献， 发育、行为、衰老、疾病等许多正常和病理生理过程。更多 最近，苍蝇模型也被用来探索环境因素对健康和健康的贡献 疾病。在被调查的环境因素中，关键是饮食。改变苍蝇的饮食具有深远的意义 对他们的生理、疾病标志物进展和衰老的影响。例如，对苍蝇的饮食限制 (通过降低营养浓度)延长寿命，就像在其他物种中发现的那样。苍蝇饮食的变化 也极大地影响产卵(资源利用的一个指标)，并可能导致II型 糖尿病样状态。此外，我们的初步研究表明，饮食对酒精有明显的影响。 苍蝇的镇静行为。考虑到果蝇饮食的影响，正在进行的全球肥胖症流行和 肥胖相关疾病发病率的增加，对果蝇的研究为继续 揭示饮食相关现象背后的关键机制，最终可能转化为改善 预防和治疗人类多种与饮食有关的疾病。因此，我们已经开始 开发基于染料的方法(CON-Ex)来测量苍蝇的食物消耗。的首要目标是 本申请中的研究是：(I)使用我们先前建立的基于染料的CON-Ex加料方法 染料Blue-1作为食物示踪剂来评估延年益寿的饮食摄入量--重要的是 苍蝇的寿命和(Ii)通过鉴定和验证除Blue-1之外的染料来扩展该方法的实用性 用于CON-Ex研究。拟议的研究将解决与饮食影响有关的一个重要问题。 并将为更复杂的苍蝇饮食消耗分析奠定基础。