Small and large animal surgery, physiology and histology

项目总结 心脏生理学和外科核心(核心C)将是该计划的重点，通过提供 基础设施，以支持许多实验方法，以评估心脏生理学和病理学， 完好无损的动物。利用联合主任的丰富经验和专门知识，并充分发展和 在多种动物模型中评估心脏保护的成熟协议，Core不仅将 提供一致和可重复的服务来支持项目，但将进一步参与 实验设计、资源的优先排序和数据的解释。此外，核心C将确定 随着计划的成熟，在所有项目之间进行协作的其他领域，从而成为 在生成数据时，对整个平台进行交互环境和全面分析。这个 Science Core将提供与该计划的全面成功相关的广泛服务，包括 在小鼠和猪模型中：将新的无细胞外切体疗法输送到心肌，在 在体脑缺血/再灌流损伤模型的建立及其对心脏结构和功能的评价 方法学，以及心肌损伤的组织学和生化评估。这些服务将提供 本计划的主要重点是骨干项目拨款，以确定外周体疗法的影响 心肌缺血再灌注损伤的分子发病机制。

PROJECT SUMMARY The Cardiac Physiology and Surgical Core (Core C) will be a focal point of the program by providing the infrastructure to support numerous experimental approaches to assess cardiac physiology and pathology, in the intact animal. Utilizing the vast experience and expertise of the Co-Director's, and well developed and proven protocols for the assessment of cardioprotection in multiple animal models, the Core will not only provide a consistent and reproducible service in support of the projects but will further participate in experimental design, prioritization of resources, and the interpretation of data. In addition, Core C will identify additional areas for collaboration across all projects as the program matures and thus be central to the interactive environment and comprehensive analysis of the overall platform as data is generated. The scientific core will provide a wide-range of services pertinent to the overall success of the program including in both mouse and porcine models: delivery of novel cell-free exosome therapeutics to the myocardium, in vivo induction of ischemia/reperfusion (IR) injury, the assessment of cardiac structure and function by multiple methodologies, and histological and biochemical assessment of myocardial injury. These services will provide the backbone for the major focus of this Program Project Grant to determine the impact of exosome therapy on the molecular pathogenesis of cardiac IR-injury.