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Small and large animal surgery, physiology and histology

小型和大型动物外科、生理学和组织学

基本信息

批准号：
9980466
负责人：
John William Elrod
金额：
$ 53.36万
依托单位：
TEMPLE UNIV OF THE COMMONWEALTH
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/9980466
关键词：
Anatomy Animal Model Animals Area Balloon Angioplasty Biochemical Biological Assay Blinded Cardiac Cell Therapy Cells Clinic Collaborations Consultations Coronary artery Coupling Custom Data Data Analyses Evaluation Experimental Designs Family suidae Fostering Functional disorder Goals Gold Heart Diseases Heart Injuries Histologic Histology Human Infrastructure Injury Investigation Ischemia Left Ligation Mediator of activation protein Methodology Miniature Swine Modeling Molecular Molecular Mechanisms of Action Mus Myocardial Myocardial Infarction Myocardial Ischemia Myocardial dysfunction Myocardium Nodal Operative Surgical Procedures Pathogenesis Pathology Patients Phenotype Physiology Population Process Productivity Program Development Program Research Project Grants Protocols documentation Reperfusion Injury Reperfusion Therapy Reproducibility Research Design Research Personnel Resources Services Signal Transduction Structure Surgical Models Techniques Testing Therapeutic Translations Treatment Efficacy Variant Vertebral column cardioprotection collaborative environment exosome experience experimental study heart function in vivo in vivo Model injury and repair mouse model myocardial injury novel paracrine pre-clinical programs repaired stem cells success synergism treatment strategy

项目摘要

PROJECT SUMMARY The Cardiac Physiology and Surgical Core (Core C) will be a focal point of the program by providing the infrastructure to support numerous experimental approaches to assess cardiac physiology and pathology, in the intact animal. Utilizing the vast experience and expertise of the Co-Director's, and well developed and proven protocols for the assessment of cardioprotection in multiple animal models, the Core will not only provide a consistent and reproducible service in support of the projects but will further participate in experimental design, prioritization of resources, and the interpretation of data. In addition, Core C will identify additional areas for collaboration across all projects as the program matures and thus be central to the interactive environment and comprehensive analysis of the overall platform as data is generated. The scientific core will provide a wide-range of services pertinent to the overall success of the program including in both mouse and porcine models: delivery of novel cell-free exosome therapeutics to the myocardium, in vivo induction of ischemia/reperfusion (IR) injury, the assessment of cardiac structure and function by multiple methodologies, and histological and biochemical assessment of myocardial injury. These services will provide the backbone for the major focus of this Program Project Grant to determine the impact of exosome therapy on the molecular pathogenesis of cardiac IR-injury.

项目概要心脏生理学和外科核心（核心 C）将成为该计划的重点，通过提供支持多种评估心脏生理学和病理学的实验方法的基础设施完整的动物。利用联合董事的丰富经验和专业知识，以及良好的发展和用于在多种动物模型中评估心脏保护作用的经过验证的方案，核心不仅提供一致且可重复的服务来支持项目，但将进一步参与实验设计、资源优先级和数据解释。此外，Core C 将识别随着该计划的成熟，所有项目之间可以进行更多的合作，从而成为该计划的核心交互环境和数据生成时对整个平台的综合分析。这科学核心将提供与该计划总体成功相关的广泛服务，包括在小鼠和猪模型中：将新型无细胞外泌体疗法递送至心肌，体内诱导缺血/再灌注（IR）损伤，通过多种方法评估心脏结构和功能心肌损伤的方法学、组织学和生化评估。这些服务将提供该计划主要重点的支柱项目拨款以确定外泌体疗法的影响心脏IR损伤的分子发病机制。