Small and large animal surgery, physiology and histology

项目概要 心脏生理学和外科核心（核心 C）将成为该计划的重点，通过提供 支持多种评估心脏生理学和病理学的实验方法的基础设施 完整的动物。利用联合董事的丰富经验和专业知识，以及良好的发展和 用于在多种动物模型中评估心脏保护作用的经过验证的方案，核心不仅 提供一致且可重复的服务来支持项目，但将进一步参与 实验设计、资源优先级和数据解释。此外，Core C 将识别 随着该计划的成熟，所有项目之间可以进行更多的合作，从而成为该计划的核心 交互环境和数据生成时对整个平台的综合分析。的 科学核心将提供与该计划总体成功相关的广泛服务，包括 在小鼠和猪模型中：将新型无细胞外泌体疗法递送至心肌， 体内诱导缺血/再灌注（IR）损伤，通过多种方法评估心脏结构和功能 心肌损伤的方法学、组织学和生化评估。这些服务将提供 该计划主要重点的支柱 项目拨款以确定外泌体疗法的影响 心脏IR损伤的分子发病机制。

PROJECT SUMMARY The Cardiac Physiology and Surgical Core (Core C) will be a focal point of the program by providing the infrastructure to support numerous experimental approaches to assess cardiac physiology and pathology, in the intact animal. Utilizing the vast experience and expertise of the Co-Director's, and well developed and proven protocols for the assessment of cardioprotection in multiple animal models, the Core will not only provide a consistent and reproducible service in support of the projects but will further participate in experimental design, prioritization of resources, and the interpretation of data. In addition, Core C will identify additional areas for collaboration across all projects as the program matures and thus be central to the interactive environment and comprehensive analysis of the overall platform as data is generated. The scientific core will provide a wide-range of services pertinent to the overall success of the program including in both mouse and porcine models: delivery of novel cell-free exosome therapeutics to the myocardium, in vivo induction of ischemia/reperfusion (IR) injury, the assessment of cardiac structure and function by multiple methodologies, and histological and biochemical assessment of myocardial injury. These services will provide the backbone for the major focus of this Program Project Grant to determine the impact of exosome therapy on the molecular pathogenesis of cardiac IR-injury.