Small and large animal surgery, physiology and histology

项目摘要 心脏生理学和外科核心（核心C）将成为该计划的焦点， 基础设施，以支持众多的实验方法，以评估心脏生理学和病理学， 完整的动物。利用共同主任的丰富经验和专门知识， 在多种动物模型中评估心脏保护的成熟方案，核心不仅 提供一致和可复制的服务，以支持项目，但将进一步参与 实验设计、资源的优先排序和数据的解释。此外，核心C将确定 随着计划的成熟，所有项目之间的合作领域将增加，从而成为 交互式环境和数据生成时对整个平台的综合分析。的 科学核心将提供与该计划的总体成功相关的广泛服务，包括 在小鼠和猪模型中：将新的无细胞外泌体治疗剂递送至心肌， 体内诱导缺血/再灌注（IR）损伤，通过多项心脏结构和功能评估， 方法学以及心肌损伤的组织学和生物化学评估。这些服务将提供 该计划项目资助的主要重点是确定外泌体治疗的影响 心脏IR损伤的分子发病机制。

PROJECT SUMMARY The Cardiac Physiology and Surgical Core (Core C) will be a focal point of the program by providing the infrastructure to support numerous experimental approaches to assess cardiac physiology and pathology, in the intact animal. Utilizing the vast experience and expertise of the Co-Director's, and well developed and proven protocols for the assessment of cardioprotection in multiple animal models, the Core will not only provide a consistent and reproducible service in support of the projects but will further participate in experimental design, prioritization of resources, and the interpretation of data. In addition, Core C will identify additional areas for collaboration across all projects as the program matures and thus be central to the interactive environment and comprehensive analysis of the overall platform as data is generated. The scientific core will provide a wide-range of services pertinent to the overall success of the program including in both mouse and porcine models: delivery of novel cell-free exosome therapeutics to the myocardium, in vivo induction of ischemia/reperfusion (IR) injury, the assessment of cardiac structure and function by multiple methodologies, and histological and biochemical assessment of myocardial injury. These services will provide the backbone for the major focus of this Program Project Grant to determine the impact of exosome therapy on the molecular pathogenesis of cardiac IR-injury.