KULMAP: Human Kidney, urinary tract and lung mapping center

KULMAP：人类肾脏、泌尿道和肺部绘图中心

• 批准号: 9987373
• 负责人: James S. Hagood
• 金额: $ 10万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9987373
• 关键词: Addendum; Address; Administrative Supplement; Adult; Altruism; Atlases; Authorization documentation; Autopsy; Award; Biological Assay; Biopsy; Bladder; Blood; Cell Culture Techniques; Cell Nucleus; Cell physiology; Cells; Childhood; Chromatin; Chromosomes; Clinical; Consent; Cryopreservation; DNA Methylation; Data; Databases; Disease; Equipment and supply inventories; Ethnic Origin; Florida; Foundations; Funding; Gene Expression; Gene Proteins; Genomics; Gifts; Health; Heart; High Resolution Computed Tomography; Human; Image; Immunofluorescence Immunologic; Immunohistochemistry; Infrastructure; Institutes; International; Investigation; Kidney; Letters; Lipids; Lower urinary tract; Lung; Lung diseases; Magnetic Resonance Imaging; Maps; Mediastinal; Mediation; Medicine; Metadata; Molecular; Morphologic artifacts; Nature; Organ; Organ Procurements; Organ Transplantation; Perfusion; Process; Proteins; Proteomics; Protocols documentation; Publications; RNA; Race; Recording of previous events; Recovery; Research; Research Personnel; Research Support; Rights; Shipping; Specificity; Spleen; Standardization; Submersion; Testing; Three-dimensional analysis; Thymus Gland; Time; Tissue Procurements; Tissues; Transplantation; Universities; Ureter; Urinary tract; Variant; Washington; Work; biobank; clinically relevant; computer framework; data access; data sharing; genomic data; human tissue; improved; in situ imaging; interest; lymph nodes; multiple omics; peripheral blood; programs; repository; sex; success; technology development; technology validation; transcriptome sequencing; transcriptomics; virtual; willingness

ABSTRACT This Administrative Supplement application will add to the original aims of the UCSD HuBMAP KULMAP award, which are to: (1) Aim 1: Establish an experimental and computational pipeline for generating and analyzing 3D multi-omics maps for human tissues that will include single-nucleus RNA sequencing and chromatin accessibility mapping, highly multiplexed in situ imaging of RNA and proteins, and a computational framework to integrate different types of data to create coherent spatial maps of RNA, chromatin status and proteins; (2) Aim 2: Create a Lung Airways and Parenchymal Map (LAPMAP), covering the entire intrathoracic human airway; and (3) Aim 3: Create Bladder, Ureter and Kidney Maps (BUKMAP), covering human kidney and lower urinary tract. Our proposed supplemental work will improve the authorization/consent process by which the tissues are acquired for KULMAP, better inform the authorizing next of kin and provide clearer definitions and guides for investigator release of data, especially sequencing data, into the pipeline. In addition, testing variations in recovery protocols and comparing analysis results generated on the organs uniformly collected and shared between TMCs will clearly inform and optimize the quality and reliability of the data generated by KULMAP, and, importantly, contribute to the overall impact and success of HuBMAP. The Aims of the Supplement are ambitious but with the currently demonstrated support of the research recovery organizations, UCSD, and the several HuBMAP TMCs, they are feasible in the one-year award period with potential for long-term impact on human organ research. The Aims are to (1) develop a Donor Authorization Addendum acceptable to the HuBMAP Consortium and broadly to the Organ Procurement Organizations, that will directly address open-access genomic data sharing but that will ultimately broaden the diversity of donors available to the Consortium; (2) standardize and promote recovery of multiple, high quality organs and tissues appropriate for state-of-the-art molecular and cellular analyses and imaging to be applied in HuBMAP, to include testing of variations in recovery protocols on experimental quality; (3) promote sharing of single- donor, multiple organ recovery biospecimens among Tissue Mapping Centers, Transformative Technology Development Centers and Rapid Implementation of Technology Centers; and (4) promote ease and accuracy of donor health history metadata transfer to the HuBMAP database.

抽象的 此行政补充申请将添加到UCSD Hubmap Kulmap的原始目的中 奖励，即：（1）目标1：建立一个实验和计算管道来生成和 分析将包括单核RNA测序和 染色质可访问性映射，高度多路复用的RNA和蛋白质的原位成像以及计算 集成不同类型的数据以创建RNA，染色质状态和 蛋白质； （2）AIM 2：创建肺气道和实质图（LaPmap），覆盖整个静脉曲张 人类气道； （3）目标3：创建膀胱，输尿管和肾脏地图（bukmap），覆盖人类肾脏 并降低尿路。我们提出的补充工作将通过 为kulmap购买了哪些组织，更好地告知授权的亲属并提供更清晰的 调查员释放数据，尤其是测序数据的定义和指南中。在 此外，测试恢复协议中的变化和比较器官生成的分析结果 TMC之间统一的收集和共享将清楚地告知并优化 Kulmap产生的数据，重要的是，有助于Hubmap的整体影响和成功。这 补充的目的是雄心勃勃的，但目前证明了研究恢复的支持 组织，UCSD和几个Hubmap TMCS，它们在一年的奖励期内是可行的 长期影响人体器官研究的潜力。目的是（1）建立捐助者授权 Hubmap联盟可接受的附录以及广泛地接受器官采购组织， 这将直接解决开放访问基因组数据共享，但最终将扩大 可用于财团的捐助者； （2）标准化和促进恢复多个高质量器官的恢复 适用于最先进的分子和细胞分析和成像的组织，用于Hubmap， 包括测试实验质量恢复方案中的变化； （3）促进单一共享 供体，多器官恢复生物测量中心之间的生物测量，变革技术 发展中心和技术中心的快速实施； （4）提高轻松和准确性 捐赠者健康历史元数据转移到Hubmap数据库。