KULMAP: Human Kidney, urinary tract and lung mapping center

KULMAP：人类肾脏、泌尿道和肺部绘图中心

• 批准号: 9987373
• 负责人: James S. Hagood
• 金额: $ 10万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9987373
• 关键词: Addendum; Address; Administrative Supplement; Adult; Altruism; Atlases; Authorization documentation; Autopsy; Award; Biological Assay; Biopsy; Bladder; Blood; Cell Culture Techniques; Cell Nucleus; Cell physiology; Cells; Childhood; Chromatin; Chromosomes; Clinical; Consent; Cryopreservation; DNA Methylation; Data; Databases; Disease; Equipment and supply inventories; Ethnic Origin; Florida; Foundations; Funding; Gene Expression; Gene Proteins; Genomics; Gifts; Health; Heart; High Resolution Computed Tomography; Human; Image; Immunofluorescence Immunologic; Immunohistochemistry; Infrastructure; Institutes; International; Investigation; Kidney; Letters; Lipids; Lower urinary tract; Lung; Lung diseases; Magnetic Resonance Imaging; Maps; Mediastinal; Mediation; Medicine; Metadata; Molecular; Morphologic artifacts; Nature; Organ; Organ Procurements; Organ Transplantation; Perfusion; Process; Proteins; Proteomics; Protocols documentation; Publications; RNA; Race; Recording of previous events; Recovery; Research; Research Personnel; Research Support; Rights; Shipping; Specificity; Spleen; Standardization; Submersion; Testing; Three-dimensional analysis; Thymus Gland; Time; Tissue Procurements; Tissues; Transplantation; Universities; Ureter; Urinary tract; Variant; Washington; Work; biobank; clinically relevant; computer framework; data access; data sharing; genomic data; human tissue; improved; in situ imaging; interest; lymph nodes; multiple omics; peripheral blood; programs; repository; sex; success; technology development; technology validation; transcriptome sequencing; transcriptomics; virtual; willingness

ABSTRACT This Administrative Supplement application will add to the original aims of the UCSD HuBMAP KULMAP award, which are to: (1) Aim 1: Establish an experimental and computational pipeline for generating and analyzing 3D multi-omics maps for human tissues that will include single-nucleus RNA sequencing and chromatin accessibility mapping, highly multiplexed in situ imaging of RNA and proteins, and a computational framework to integrate different types of data to create coherent spatial maps of RNA, chromatin status and proteins; (2) Aim 2: Create a Lung Airways and Parenchymal Map (LAPMAP), covering the entire intrathoracic human airway; and (3) Aim 3: Create Bladder, Ureter and Kidney Maps (BUKMAP), covering human kidney and lower urinary tract. Our proposed supplemental work will improve the authorization/consent process by which the tissues are acquired for KULMAP, better inform the authorizing next of kin and provide clearer definitions and guides for investigator release of data, especially sequencing data, into the pipeline. In addition, testing variations in recovery protocols and comparing analysis results generated on the organs uniformly collected and shared between TMCs will clearly inform and optimize the quality and reliability of the data generated by KULMAP, and, importantly, contribute to the overall impact and success of HuBMAP. The Aims of the Supplement are ambitious but with the currently demonstrated support of the research recovery organizations, UCSD, and the several HuBMAP TMCs, they are feasible in the one-year award period with potential for long-term impact on human organ research. The Aims are to (1) develop a Donor Authorization Addendum acceptable to the HuBMAP Consortium and broadly to the Organ Procurement Organizations, that will directly address open-access genomic data sharing but that will ultimately broaden the diversity of donors available to the Consortium; (2) standardize and promote recovery of multiple, high quality organs and tissues appropriate for state-of-the-art molecular and cellular analyses and imaging to be applied in HuBMAP, to include testing of variations in recovery protocols on experimental quality; (3) promote sharing of single- donor, multiple organ recovery biospecimens among Tissue Mapping Centers, Transformative Technology Development Centers and Rapid Implementation of Technology Centers; and (4) promote ease and accuracy of donor health history metadata transfer to the HuBMAP database.

摘要 此行政补充申请将增加UCSD HuBMAP KULMAP的原始目标 奖项，其目的是：(1)目标1：建立一个实验和计算渠道，以产生和 分析人体组织的3D多组学图谱，包括单核RNA测序和 染色质可及性图谱，RNA和蛋白质的高度多重原位成像，以及计算 集成不同类型数据的框架，以创建RNA、染色质状态和 蛋白质；(2)目标2：创建覆盖整个胸腔的肺气道和实质地图(LAPMAP) 目标3：创建覆盖人体肾脏的膀胱、输尿管和肾脏地图(BUKMAP) 和下尿路。我们建议的补充工作将通过以下方式改进授权/同意流程 哪些组织是为KULMAP获得的，更好地通知授权的近亲，并提供更清楚的 调查员将数据，特别是测序数据发布到管道中的定义和指南。在……里面 此外，测试回收方案的变化并比较对器官产生的分析结果 统一收集并在TMC之间共享将清楚地告知和优化 KULMAP产生的数据，以及更重要的是，有助于HuBMAP的整体影响和成功。这个 补编的目标是雄心勃勃的，但目前已证明支持研究复苏 组织、UCSD和几个HuBMAP TMC，它们在一年的获奖期内是可行的，具有 可能对人体器官研究产生长期影响。其目的是(1)开发捐赠者授权 HuBMAP联合体和器官采购组织普遍可接受的增编， 这将直接解决开放获取的基因组数据共享问题，但这最终将扩大 向财团提供捐赠者；(2)标准化和促进多个高质量器官的回收 适合用于HuBMAP的最先进的分子和细胞分析和成像的组织， 包括对恢复方案中对实验质量的变化进行测试；(3)促进单一- 供体，组织映射中心间的多器官回收生物标本，变革性技术 开发中心和技术中心的快速实施；以及(4)促进易用性和准确性 将捐赠者的健康历史元数据传输到HuBMAP数据库。