Higher Precision Human and Mouse Transcriptomes

项目摘要/摘要 据说，如果基因组代表了细胞所能表达的一系列东西，那么转录组 表示单元格当前所说的一组内容。转录组是主要读出的 基因组，主要由mRNAs、长的非编码RNA和microRNAs组成。在生命的所有阶段，在 在健康和疾病中，转录组的差异因果地定义和区分每种细胞类型和细胞 州政府。ENCODE联盟的使命是发现、绘制和定义所有基因及其调控 元素。因此，RNA-Seq数据和由此产生的转录本一直是先前 编码阶段，我们的团队已经贡献了超过350个释放的聚(A)mRNA和microRNA 项目的数据集。尽管这些数据有价值和高质量，但我们相信新技术将使我们能够 制作新一代的抄本，它的信息要丰富得多，也更明确。具体来说， 它们分解组成复杂组织的多种不同类型的细胞，并分解分子 数据中的同种异构体。他们定性地记录了远程单分子剪接和末端加工以及 我们提供microRNAs的伴随量化，以及发现其通常未记录在案的前体 RNA。最后，我们在体内将RNA二级结构映射到转录组。我们建议捐献 包含这些测量类型及其综合分析的300个新的、更高精度的转录本。 这项研究的一部分集中在人类和小鼠的衰老上，这是一个以前没有过的整个生命周期阶段 已在Encode中进行了研究。相应的疾病成分会将认知正常的老年人与 阿尔茨海默氏症是大脑和运动系统中的痴呆症。 。 。

PROJECT SUMMARY/ABSTRACT It is said that if the genome represents the set of things that the cell could say, then the transcriptome represents the set of things that the cell is saying right now. The transcriptome is the primary read-out of the genome and is composed mainly of mRNAs, long noncoding RNAs, and microRNAs. At all stages of life, in health and in disease, differences in the transcriptome causally define and distinguish each cell type and cell state. The mission of the ENCODE Consortium is to discover, map and define all genes and their regulatory elements. RNA-Seq data and the resulting transcriptomes have therefore been a prominent component of prior phases of ENCODE, and our groups have contributed over 350 released poly(A) mRNA and microRNA datasets for the project. Valuable and high quality as those data are, we are new technologies will allow us to produce a new generation of transcriptomes that are much more information rich and definitive. Specifically, they resolve the multiple different cell types that comprise complex tissues, and they resolve the molecular isoforms in the data. They qualitatively document long-range single molecule splicing and end processing and we provide companion quantification of microRNAs, plus discovery of their often-undocumented precursor RNAs. Finally, we map RNA secondary structure across the transcriptome in vivo. We propose to contribute 300 new, higher precision transcriptomes containing these measurement types and their integrated analysis. A portion of this study focuses on aging humans and mice, a whole life cycle stage that has not previously been studied in ENCODE. The corresponding disease component will contrast the cognitively normal old with Alzheimers dementia in the brain and in the motor system. . .