Core D - Energy Balance and Body Composition Core

能量平衡和身体成分核心(核心D)为整个机构的调查人员提供服务 量化小鼠的能量平衡和身体成分。这些参数的准确量化是 对于专注于肥胖的研究至关重要，具有广泛的新陈代谢相关研究能力。这个 核心拥有24个LabMaster TSE®实验室，可量化食物和水的摄入量、体力活动和间接 小鼠的热量测定，这是一种常见的研究模型，主要研究饮食或遗传对发育的影响 肥胖症。Core还配备了EchoMRI®，用于量化精益和脂肪质量以及体内总水分 清醒的老鼠。这两个系统都允许对能量平衡和身体进行非侵入性纵向评估 组成。称职的核心员工维护和监督LabMaster TSE®系统和培训的使用 使用EchoMRI®的人员。核心董事协助研究设计以及数据处理和分析。 每年有50多名首席研究人员使用LabMaster TSE®系统对700只小鼠进行研究，而 EchoMRI®每年被超过25个实验室使用。这些系统不会在 机构或在英联邦内，对我们减少肥胖流行的能力至关重要。 肯塔基州。在该计划的第三阶段，我们将扩大我们的重点，将肥胖作为 心血管疾病、糖尿病、癌症和神经退行性疾病。这将增加对 系统支持预期的更大用户群，并在这些领域内实现目标增长 机构。我们还将从机构支持中购买一个新系统，该系统将显著扩大 这一核心在量化小鼠能量代谢方面的能力。此外，在计划的第三阶段， 该系统的核心，包括所有设备，将搬迁到一个新的 致力于研究这些健康差距的研究大楼。我们预计这个核心将 在COCVD第三阶段支助过程中向自给自足能力过渡。

The Energy Balance and Body Composition Core (Core D) serves investigators across the institution to quantify energy balance and body composition in mice. Accurate quantification of these parameters is essential for studies focused on obesity, with wide-spread capabilities for metabolism-related studies. The core houses 24 LabMaster TSE® chambers that quantify food and water intake, physical activity and indirect calorimetry in mice, a common model for studies focused on the effect of diet or genetics on the development of obesity. The Core also houses an EchoMRI® to quantify lean and fat mass and total body water in conscious mice. Both systems allow for non-invasive longitudinal assessment of energy balance and body composition. Competent core staff maintain and oversee the use of the LabMaster TSE® system and train personnel in use of the EchoMRI®. Core Directors assist in study design and data handling and analysis. Over 50 Principal Investigators use the LabMaster TSE® system annually for studies on >700 mice/year, while the EchoMRI® is used by more than 25 laboratories annually. These systems are not duplicated at the institution or within the Commonwealth, and are vital to our ability to reduce the epidemic of obesity in Kentucky. In Phase III of the program, we will broaden our focus to include obesity as a risk factor for cardiovascular diseases, diabetes, cancer and neurodegenerative diseases. This will increase use of the system to support an anticipated larger user base with targeted growth within these areas across the institution. We will also purchase a new system, from institutional support, that will markedly expand capabilities of this core in quantifying energy metabolism in mice. Also, during Phase III of the program the core for this system, including all equipment, will be relocated to a modern animal care facility within a new research building that is dedicated to the study of these health disparities. We anticipate that this core will transition to self-sustaining capacity over the course of Phase III support of the COCVD.