Core D - Energy Balance and Body Composition Core

核心 D - 能量平衡和身体成分核心

• 批准号: 10458566
• 负责人: Changcheng Zhou
• 金额: $ 19.13万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10458566
• 关键词: Acclimatization; Adipose tissue; Animals; Area; Blood Pressure; Body Composition; Body Water; Body Weight; Cardiovascular Diseases; Cells; Chronic; Collaborations; Communities; Conscious; Consultations; Coupled; Data Analyses; Diabetes Mellitus; Diet; Eating; Energy Metabolism; Equipment; Exercise; Experimental Models; Fatty acid glycerol esters; Fees; Food Energy; Fostering; Funding; Genetic; Genome; Growth; Health care facility; Human Resources; Indirect Calorimetry; Individual; Institution; Intervention; Kentucky; Laboratories; Longitudinal Studies; Magnetic Resonance Imaging; Malignant Neoplasms; Measurement; Metabolism; Methods; Modernization; Mus; Neurodegenerative Disorders; Obese Mice; Obesity; Obesity Epidemic; Obesity associated disease; Phase; Physical activity; Pilot Projects; Principal Investigator; Proteins; Recommendation; Regulation; Research; Research Design; Research Personnel; Resources; Running; Services; Standardization; Structure; Study models; System; Therapeutic; Thinness; Time; Tissues; Training; United States National Institutes of Health; Water consumption; Weight; animal care; base; cardiovascular risk factor; data handling; diet-induced obesity; dietary; energy balance; experience; feeding; health disparity; improved; insight; mid-career faculty; obesity development; operation; outreach program; programs; time use

The Energy Balance and Body Composition Core (Core D) serves investigators across the institution to quantify energy balance and body composition in mice. Accurate quantification of these parameters is essential for studies focused on obesity, with wide-spread capabilities for metabolism-related studies. The core houses 24 LabMaster TSE® chambers that quantify food and water intake, physical activity and indirect calorimetry in mice, a common model for studies focused on the effect of diet or genetics on the development of obesity. The Core also houses an EchoMRI® to quantify lean and fat mass and total body water in conscious mice. Both systems allow for non-invasive longitudinal assessment of energy balance and body composition. Competent core staff maintain and oversee the use of the LabMaster TSE® system and train personnel in use of the EchoMRI®. Core Directors assist in study design and data handling and analysis. Over 50 Principal Investigators use the LabMaster TSE® system annually for studies on >700 mice/year, while the EchoMRI® is used by more than 25 laboratories annually. These systems are not duplicated at the institution or within the Commonwealth, and are vital to our ability to reduce the epidemic of obesity in Kentucky. In Phase III of the program, we will broaden our focus to include obesity as a risk factor for cardiovascular diseases, diabetes, cancer and neurodegenerative diseases. This will increase use of the system to support an anticipated larger user base with targeted growth within these areas across the institution. We will also purchase a new system, from institutional support, that will markedly expand capabilities of this core in quantifying energy metabolism in mice. Also, during Phase III of the program the core for this system, including all equipment, will be relocated to a modern animal care facility within a new research building that is dedicated to the study of these health disparities. We anticipate that this core will transition to self-sustaining capacity over the course of Phase III support of the COCVD.

能量平衡和身体成分核心（核心D）为整个机构的研究人员提供服务， 量化小鼠的能量平衡和身体组成。这些参数的精确量化是 对于肥胖症研究至关重要，具有广泛的代谢相关研究能力。的 核心包含24个LabMaster TSE®腔室，可量化食物和水的摄入量、身体活动和间接 量热法在小鼠中，一种常见的模型，研究重点是饮食或遗传学对发育的影响， 肥胖症。Core还配备了EchoMRI®，可量化瘦体重和脂肪量以及体内总水分， 清醒的老鼠这两种系统都允许对能量平衡和身体进行无创纵向评估。 混合物.称职的核心员工维护和监督LabMaster TSE®系统的使用并进行培训 使用EchoMRI®的人员。核心主任协助研究设计和数据处理与分析。 超过50名主要研究者每年使用LabMaster TSE®系统进行超过700只小鼠的研究， EchoMRI®每年被超过25个实验室使用。这些系统在 机构或英联邦内部，并对我们减少肥胖症流行的能力至关重要， 肯塔基州。在该计划的第三阶段，我们将扩大我们的重点，包括肥胖作为一个危险因素， 心血管疾病、糖尿病、癌症和神经变性疾病。这将增加使用 系统，以支持预期的更大的用户群，并在这些领域实现目标增长 机构。我们还将从机构支持中购买一个新系统， 这个核心在量化小鼠能量代谢方面的能力。此外，在该计划的第三阶段， 该系统的核心，包括所有设备，将被重新安置在一个新的现代化的动物护理设施， 这座研究大楼致力于研究这些健康差异。我们预计，这一核心将 在COCVD第三阶段支助过程中过渡到自我维持能力。