Chemoenzymatic synthesis of bacterial polysaccharides
细菌多糖的化学酶法合成
基本信息
- 批准号:9981827
- 负责人:
- 金额:$ 72.04万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2017
- 资助国家:美国
- 起止时间:2017-09-01 至 2022-07-31
- 项目状态:已结题
- 来源:
- 关键词:ATP-Binding Cassette TransportersAddressAdhesionsAnabolismBacteriaBacterial PolysaccharidesBiologicalBiomedical ResearchCell surfaceChondroitinCommunicable DiseasesComplexDevelopmentDiagnosisDiagnosticDiphosphatesEnzymesGalactansGenerationsGenus MycobacteriumGlycobiologyHigh Pressure Liquid ChromatographyHumanImmune systemImmunomodulatorsIn VitroIndividualLaboratoriesLengthLipidsMediatingMediator of activation proteinMonosaccharidesOligosaccharidesPaperPathogenicityPathway interactionsPolymerasePolysaccharidesPreparationProductionProtocols documentationPublicationsReactionReagentResearchResearch PersonnelStructureSystemTechnologyVaccine DesignVaccinesValidationVariantVirulence Factorsbasecarbohydrate binding proteinchemical synthesisexperiencelipooligosaccharidemedical specialtiesmicrobialpolymerizationprogramsreconstitutionresearch and developmentweb site
项目摘要
Chemoenzymatic synthesis of bacterial polysaccharides
Project Summary
The bacterial cell surface is decorated with remarkable variations of polysaccharide structures including capsular
polysaccharides (CPS), O-polysaccharides (O-PS), and exopolysaccharides (EPS). These polysaccharides
mediate interactions between the bacterium and its host. Many are important virulence factors, adhesion
mediators, or immunomodulators. Because their composition differs dramatically from that of host glycans,
multiple bacterial polysaccharides have been used to develop vaccines to protect human from a diverse array of
bacterial infectious diseases. To date, these polysaccharides have been commonly isolated from the bacterial
cultures and therefore consist of heterogeneous mixtures. Structurally defined polysaccharides with defined
numbers of repeating units are not readily available. With the combined expertise of three groups on efficient
one-pot multienzyme chemoenzymatic synthesis of oligosaccharide repeating units, polymerization of
lipooligosaccharide repeating units for the formation of Wzy-dependent bacterial polysaccharides and
polysaccharide synthase-catalyzed production of bacterial polysaccharides, structurally defined polysaccharides
with designated numbers of oligosaccharide repeating units will be synthesized. These compounds are valuable
probes for glycan microarray studies and candidates for the development of diagnostics, immunomodulators,
and vaccines. Optimal storage and reaction conditions will be identified. Enzymes and reagents will be
assembled in convenient-to-store and easy-to-use kits. Protocols for synthesis and purification will be
established and shared by publication of papers and on a designated website. These kits will allow
non-specialists to carry out the synthesis.
细菌多糖的化学酶合成
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Laura L Kiessling其他文献
Laura L Kiessling的其他文献
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{{ truncateString('Laura L Kiessling', 18)}}的其他基金
The Chemistry and Biology of Galactofuranose-Containing Glycans
含呋喃半乳糖聚糖的化学和生物学
- 批准号:
9528179 - 财政年份:2017
- 资助金额:
$ 72.04万 - 项目类别:
Chemoenzymatic synthesis of bacterial polysaccharides
细菌多糖的化学酶法合成
- 批准号:
9764158 - 财政年份:2017
- 资助金额:
$ 72.04万 - 项目类别:
TRAINING IN THE USE OF BRUKER AND VARIAN SPECTROMETERS AND NMR
布鲁克和瓦里安光谱仪和核磁共振的使用培训
- 批准号:
8361174 - 财政年份:2011
- 资助金额:
$ 72.04万 - 项目类别:
MECHANISTIC INVESTIGATION OF THE MYCOBACTERIAL GLYCOSYLTRANSFERASE GLFT2
分枝杆菌糖基转移酶 GLFT2 的机制研究
- 批准号:
8361170 - 财政年份:2011
- 资助金额:
$ 72.04万 - 项目类别:
MECHANISTIC INVESTIGATION OF THE MYCOBACTERIAL GLYCOSYLTRANSFERASE
分枝杆菌糖基转移酶的机制研究
- 批准号:
8168973 - 财政年份:2010
- 资助金额:
$ 72.04万 - 项目类别:
TRAINING IN THE USE OF BRUKER AND VARIAN SPECTROMETERS AND NMR
布鲁克和瓦里安光谱仪和核磁共振的使用培训
- 批准号:
8168978 - 财政年份:2010
- 资助金额:
$ 72.04万 - 项目类别:
Glycopeptides and Other Non-Natural Variants: Probes of Carbohydrate Function
糖肽和其他非天然变体:碳水化合物功能的探针
- 批准号:
7937468 - 财政年份:2009
- 资助金额:
$ 72.04万 - 项目类别:
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