THE CHEMISTRY AND BIOLOGY OF GALACTOFURANOSE

呋喃半乳糖的化学和生物学

• 批准号: 8168936
• 负责人: Laura L Kiessling
• 金额: $ 0.03万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168936
• 关键词: Active Sites; Amino Acids; Bacteria; Binding; Binding Sites; Biology; Cell Wall; Chemistry; Computer Retrieval of Information on Scientific Projects Database; Drug Delivery Systems; Enzymes; Excision; Flavins; Funding; Galactose; Grant; Institution; Label; Mammalian Cell; Molecular Weight; Mutate; Mycobacterium tuberculosis; Oxidation-Reduction; Protocols documentation; Research; Research Personnel; Resources; Role; Source; UDP-galactopyranose mutase; United States National Institutes of Health; adduct; cofactor; uridine diphosphate galactofuranose

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The flavoenzyme UDP-galactopyranose mutase (UGM) catalyzes the interconversion of UDP-galactopyranose and UDP-galactofuranose. Galactofuranose residues, which are not found in mammalian cells, are then incorporated into the cell walls of certain bacteria, including Mycobacterium tuberculosis, making UGM an attractive drug target. UGM is a flavoenzyme, but the precise role of the cofactor has been unclear due to the lack of reduction/oxidation chemistry in this isomerization. We propose that turnover occurs by a covalent flavin-galactose iminium species. Using NaCNBH3 to trap this adduct, analysis of the resulting species by LCMS shows a peak corresponding to the molecular weight of the proposed alkylated flavin, as well as an absorbance spectrum indicative of an N(5) alkylflavin. In order to investigate how the enzyme allows for this unique chemistry, we have begun to mutate conserved residues around the active site in order to establish the mechanistic roles of certain amino acids. A protocol for removal of the noncovalently bound flavin and replacement with isotopically labeled flavin has been developed to allow for NMR studies of the flavin binding site, as well as to use NMR to visualize the connectivities of the covalent alkylflavin.

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 黄素酶UDP-吡喃半乳糖苷酶（UGM）催化UDP-吡喃半乳糖和UDP-呋喃半乳糖的相互转化。 在哺乳动物细胞中没有发现的呋喃半乳糖残基，然后被掺入某些细菌的细胞壁中，包括结核分枝杆菌，使UGM成为一个有吸引力的药物靶点。 UGM是一种黄素酶，但由于在这种异构化中缺乏还原/氧化化学，因此辅因子的确切作用尚不清楚。 我们建议，营业额发生的共价黄素-半乳糖亚胺物种。 使用NaCNBH 3捕获该加合物，通过LCMS分析所得物质显示对应于所提出的烷基化黄素的分子量的峰，以及指示N（5）烷基黄素的吸收光谱。 为了研究酶如何允许这种独特的化学反应，我们已经开始突变活性位点周围的保守残基，以建立某些氨基酸的机制作用。 已经开发了一种用于去除非共价结合的黄素并用同位素标记的黄素替换的方案，以允许黄素结合位点的NMR研究，以及使用NMR来可视化共价烷基黄素的连接性。