Proprioceptive Coding of Jaw Movement during Orofacial Behavior

项目摘要 感觉信息与运动指令的整合使运动具有适应性。比如说， 许多生存关键的口面行为（咀嚼、饮水、呼吸等）涉及更新运动 基于与物体的交互的轨迹（例如，将咀嚼模式与食物材料特性相匹配）。 本体感受器是提供有关身体位置信息的感觉传入， 在这个过程中的角色。然而，鉴于过去大多数本体感受器的记录都不是在清醒状态下进行的， 动物，清醒情况下本体感受器运动编码缺乏清晰的理解。这个项目 将从小鼠下颌神经支配的本体感受器（位于后脑中脑）靶向并记录 三叉神经（MeV）核）在口面行为，并测试其功能作用。目标1将描述 小鼠MeV传入的功能组织，同时开发针对这一人群的遗传工具。目的 2将在高分辨率运动跟踪期间记录行为动物的MeV神经元。使用此 数据集，建立一个系统的定量模型，为MeV神经元活动的自愿口面 行为目的3通过干扰MeV神经元的活动，了解MeV神经元在口面神经系统中的功能作用。 行为该项目的完成将涉及体内生理学，行为分析， 和系统/计算技术。这种训练将利用丰富的神经回路环境， 系统研究，特别是基于实验室的感觉和运动系统神经科学的专业知识， 赞助商和共同赞助商。总的来说，拟议的项目将定义信息编码属性， 在orofacial运动的本体感受器，以及探测这一信息的功能作用， 行为这些结果可以为临床上看到的下颌运动问题的治疗策略提供信息（即， 戴假牙的患者、颞下颌关节紊乱病等）。更广泛地说，这项工作可以提供新颖的 深入了解本体感受信息在运动控制中的作用，这对运动控制有着广泛的影响。 对健康和病理背景下的运动有基本的理解。

Project Summary Integration of sensory information with motor commands allows movement to be adaptable. For example, many survival-critical orofacial behaviors (chewing, drinking, breathing, etc.) involve updating movement trajectories based on interaction with objects (e.g. matching chewing patterns to food material properties). Proprioceptors, which are sensory afferents that provide information about body position, likely play critical roles in this process. However, given that most past recording of proprioceptors was not performed in awake animals, a clear understanding of proprioceptor movement coding in the awake context is lacking. This project will target and record from mouse jaw-innervating proprioceptors (which lie in the hindbrain mesencephalic trigeminal (MeV) nucleus) during orofacial behavior and test their functional role. Aim 1 will characterize the functional organization of mouse MeV afferents while developing genetic tools for targeting this population. Aim 2 will record from MeV neurons in behaving animals during high-resolution movement tracking. Using this dataset, a systematic quantitative model will be built for MeV neuron activity during voluntary orofacial behavior. Aim 3 will perturb MeV neuron activity to understand the functional role of MeV neurons in orofacial behavior. Completion of this project will involve high-level training in in vivo physiology, behavioral analysis, and systems/computational techniques. This training will draw upon a rich environment for neural circuit and systems research, especially based on the expertise in sensory and motor systems neuroscience in the labs of the Sponsor and Co-Sponsor. Collectively, the proposed project will define the information coding properties of proprioceptors during orofacial movement as well as probe the functional role of this information during behavior. These results could inform strategies for treatment of jaw motor problems seen in the clinic (i.e. denture-wearing patients, temporomandibular joint disorders, etc.). More broadly, this work can provide novel insights into the role of proprioceptive information in motor control, which has wide-ranging implications for the fundamental understanding of movement in healthy and pathological contexts.