Hyperion multi-parameter high-dimensional imaging mass cytometry platform

Hyperion多参数高维成像质谱流式分析平台

基本信息

  • 批准号:
    10193821
  • 负责人:
  • 金额:
    $ 59.99万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2021
  • 资助国家:
    美国
  • 起止时间:
    2021-04-15 至 2022-04-14
  • 项目状态:
    已结题

项目摘要

Tissue imaging is a research resource used by a broad range of investigators across the UC Irvine campus and UC Irvine Medical Center working in immunology, oncology and cell biology, including members of the Sue and Bill Gross Stem Cell Research Center (SCRC), the Institute for Clinical and Translational Science (ICTS), the Institute for Immunology, the Institute for Memory Impairments and Neurological Diseases (UCI MIND), the Cancer Research Institute (CRI) and the Chao Family Comprehensive Cancer Center (CFCCC). The SCRC maintains tissue imaging and flow cytometry facilities that are amongst the most efficiently operated and frequently utilized core services on our campus. The SCRC staff are widely consulted for their expertise as evidenced by their continuing roles in the SoCal Flow Summit meeting for southern California flow cytometry users that is held annually here at UCI. While the SCRC maintains several flow cytometers and sorters and a variety of fluorescence microscopes for tissue analyses, sorting and imaging, these instruments are limited in the number of parameters that can be assessed simultaneously. We are requesting funds for the purchase of a Fluidigm Hyperion mass imager. This instrument is a high-performance mass imager that allows for simultaneous detection of greater than 50 parameters. The instrument utilizes a time of flight mass spectrometer to detect heavy metal labeled biological probes, including antibodies, protein ligands and nucleic acid probes, allowing for simultaneous detection of a number of cellular markers. These features make the instrument particularly useful for stem cell applications, which often require analysis of multiple markers to identify particular rare cell populations. The instrument will be installed, operated and maintained by the SCRC within our core laboratory recharge facility. Since the Hyperion requires a Helios mass cytometer for functionality, the SCRC and other units on campus have joined together to fund the purchase of the Helios mass cytometer, as well as for its installation and the development of a CyTOF core that will offer services and reagents to the research community at UCI. User fees will be calculated and submitted to the UCI Budget Office for approval following all appropriate UCI policies and procedures. This new Fluidigm Hyperion will complement existing instruments by providing new experimental paradigms not currently available at UCI, enhance our translational efforts and offer unique training and outreach experiences. Thus, the purchase of a Fluidigm Hyperion will have a significant impact on the biomedical research enterprise at our campus. Within the parameters of the NIH S10 grant funding, priority usage will be given to listed major and minor users with NIH funding, but the instrument also will be available to all UCI researchers.
组织成像是加州大学欧文分校范围广泛的研究人员使用的一种研究资源 和加州大学欧文医学中心从事免疫学、肿瘤学和细胞生物学工作,包括Sue 以及比尔·格罗斯干细胞研究中心(SCRC)、临床与翻译科学研究所(ICTS)、 免疫学研究所、记忆损伤和神经疾病研究所(UCI Mind)、 癌症研究所(CRI)和赵氏家族综合癌症中心(CFCCC)。中华全国总商会 维护组织成像和流式细胞仪设施,这些设施是运行最有效和 我们校园中经常使用的核心服务。我们广泛谘询委员会职员的意见,以征询他们的专业意见,包括 他们在南加州流式细胞术的SoCal Flow峰会上的持续作用证明了这一点 UCI每年在这里举行的用户会议。虽然SCRC维护了几个流式细胞仪和分选器,以及 用于组织分析、分类和成像的各种荧光显微镜,这些仪器仅限于 可以同时评估的参数数量。我们正在申请资金,以购买一个 Fluidigm Hyperion质量成像仪该仪器是一种高性能的质量成像仪,允许 同时检测50个以上的参数。该仪器利用飞行质量时间 检测重金属标记生物探针的光谱仪,包括抗体、蛋白质配体和核酸 酸性探针,允许同时检测多个细胞标志物。这些功能使 对干细胞应用特别有用的仪器,这通常需要分析多个标记以 识别特定的稀有细胞群。该仪器将由SCRC安装、操作和维护 在我们的核心实验室充电设施内。因为Hyperion需要一台Helios质量细胞仪 功能,SCRC和校园内的其他单位已经联合起来,为购买Helios提供资金 质量细胞仪,以及它的安装和开发细胞飞行时间核心,将提供服务和 UCI研究界的试剂。用户费用将被计算并提交给UCI预算 办公室批准遵循所有适当的UCI政策和程序。这个新的海波龙将会 通过提供UCI目前没有的新实验范式来补充现有工具, 加强我们的翻译工作,并提供独特的培训和外展体验。因此,购买一个 Fluidigm Hyperion将对我们校园的生物医学研究企业产生重大影响。在 NIH S10拨款的参数,优先使用列出的主要和次要用户, NIH资助,但该仪器也将向UCI的所有研究人员提供。

项目成果

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Craig Michael Walsh其他文献

Craig Michael Walsh的其他文献

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{{ truncateString('Craig Michael Walsh', 18)}}的其他基金

Modulation of T Cell Activation and Tolerance by DRAK2
DRAK2 对 T 细胞激活和耐受性的调节
  • 批准号:
    8093107
  • 财政年份:
    2010
  • 资助金额:
    $ 59.99万
  • 项目类别:
Modulation of T Cell Activation and Tolerance by DRAK2
DRAK2 对 T 细胞激活和耐受性的调节
  • 批准号:
    7917835
  • 财政年份:
    2009
  • 资助金额:
    $ 59.99万
  • 项目类别:
Modulation of T Cell Activation and Tolerance by DRAK2
DRAK2 对 T 细胞激活和耐受性的调节
  • 批准号:
    7430248
  • 财政年份:
    2007
  • 资助金额:
    $ 59.99万
  • 项目类别:
Modulation of T Cell Activation and Tolerance by DRAK2
DRAK2 对 T 细胞激活和耐受性的调节
  • 批准号:
    7072289
  • 财政年份:
    2005
  • 资助金额:
    $ 59.99万
  • 项目类别:
GROWTH REGULATION OF ACTIVATED T CELLS BY FADD
FADD 对活化 T 细胞的生长调节
  • 批准号:
    7182391
  • 财政年份:
    2005
  • 资助金额:
    $ 59.99万
  • 项目类别:
Modulation of T Cell Activation and Tolerance by DRAK2
DRAK2 对 T 细胞激活和耐受性的调节
  • 批准号:
    6983696
  • 财政年份:
    2005
  • 资助金额:
    $ 59.99万
  • 项目类别:
Modulation of T Cell Activation and Tolerance by DRAK2
DRAK2 对 T 细胞激活和耐受性的调节
  • 批准号:
    7197336
  • 财政年份:
    2005
  • 资助金额:
    $ 59.99万
  • 项目类别:
Modulation of T Cell Activation and Tolerance by DRAK2
DRAK2 对 T 细胞激活和耐受性的调节
  • 批准号:
    7570029
  • 财政年份:
    2005
  • 资助金额:
    $ 59.99万
  • 项目类别:
Modulation of T Cell Activation and Tolerance by DRAK2
DRAK2 对 T 细胞激活和耐受性的调节
  • 批准号:
    7379929
  • 财政年份:
    2005
  • 资助金额:
    $ 59.99万
  • 项目类别:
Growth Regulation of Activated T Cells by FADD
FADD 对活化 T 细胞的生长调节
  • 批准号:
    6683606
  • 财政年份:
    2002
  • 资助金额:
    $ 59.99万
  • 项目类别:

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