Hyperion multi-parameter high-dimensional imaging mass cytometry platform

组织成像是加州大学欧文分校校园内广泛的研究人员使用的研究资源 和加州大学欧文分校医学中心在免疫学，肿瘤学和细胞生物学工作，包括苏的成员 和比尔格罗斯干细胞研究中心（SCRC），临床和转化科学研究所（ICTS）， 免疫学研究所，记忆障碍和神经疾病研究所（UCI MIND）， 癌症研究所（CRI）和Chao家庭综合癌症中心（CFCCC）。SCRC 维护组织成像和流式细胞仪设施，这些设施是最有效运行的， 经常使用的核心服务在我们的校园。SCRC工作人员因其专业知识而被广泛咨询， 他们在南加州流式细胞术的SoCal Flow Summit会议中的持续作用证明了这一点 每年在UCI举行的用户大会。虽然SCRC维护几台流式细胞仪和分选仪以及一台 用于组织分析、分选和成像的各种荧光显微镜，这些仪器在以下方面受到限制： 可以同时评估的参数数量。我们正在申请资金购买一个 Fluidigm质谱仪。该仪器是一种高性能的质谱成像仪， 同时检测50个以上参数。该仪器利用飞行时间质量 用于检测重金属标记的生物探针的光谱仪，包括抗体、蛋白质配体和核酸 酸性探针，允许同时检测许多细胞标志物。这些特性使得 本发明涉及一种特别适用于干细胞应用仪器，其通常需要分析多种标记物， 识别特定的稀有细胞群。仪器将由SCRC安装、操作和维护 在我们的核心实验室补给设施里由于细胞计数器需要Helios质量细胞计数器， 功能，SCRC和校园内的其他单位已经联合起来资助购买太阳神 质量细胞仪，以及其安装和开发的CyTOF核心，将提供服务， 向UCI的研究界提供试剂。用户费用将被计算并提交给UCI预算 根据所有适当的UCI政策和程序进行批准。这款全新的Fluidigm ® 通过提供UCI目前没有的新实验范例来补充现有的仪器， 加强我们的翻译工作，并提供独特的培训和推广经验。因此，购买A Fluidigm将对我们校园的生物医学研究企业产生重大影响。内 NIH S10赠款资金的参数，优先使用将给予列出的主要和次要用户， NIH资助，但该仪器也将提供给所有UCI研究人员。

Tissue imaging is a research resource used by a broad range of investigators across the UC Irvine campus and UC Irvine Medical Center working in immunology, oncology and cell biology, including members of the Sue and Bill Gross Stem Cell Research Center (SCRC), the Institute for Clinical and Translational Science (ICTS), the Institute for Immunology, the Institute for Memory Impairments and Neurological Diseases (UCI MIND), the Cancer Research Institute (CRI) and the Chao Family Comprehensive Cancer Center (CFCCC). The SCRC maintains tissue imaging and flow cytometry facilities that are amongst the most efficiently operated and frequently utilized core services on our campus. The SCRC staff are widely consulted for their expertise as evidenced by their continuing roles in the SoCal Flow Summit meeting for southern California flow cytometry users that is held annually here at UCI. While the SCRC maintains several flow cytometers and sorters and a variety of fluorescence microscopes for tissue analyses, sorting and imaging, these instruments are limited in the number of parameters that can be assessed simultaneously. We are requesting funds for the purchase of a Fluidigm Hyperion mass imager. This instrument is a high-performance mass imager that allows for simultaneous detection of greater than 50 parameters. The instrument utilizes a time of flight mass spectrometer to detect heavy metal labeled biological probes, including antibodies, protein ligands and nucleic acid probes, allowing for simultaneous detection of a number of cellular markers. These features make the instrument particularly useful for stem cell applications, which often require analysis of multiple markers to identify particular rare cell populations. The instrument will be installed, operated and maintained by the SCRC within our core laboratory recharge facility. Since the Hyperion requires a Helios mass cytometer for functionality, the SCRC and other units on campus have joined together to fund the purchase of the Helios mass cytometer, as well as for its installation and the development of a CyTOF core that will offer services and reagents to the research community at UCI. User fees will be calculated and submitted to the UCI Budget Office for approval following all appropriate UCI policies and procedures. This new Fluidigm Hyperion will complement existing instruments by providing new experimental paradigms not currently available at UCI, enhance our translational efforts and offer unique training and outreach experiences. Thus, the purchase of a Fluidigm Hyperion will have a significant impact on the biomedical research enterprise at our campus. Within the parameters of the NIH S10 grant funding, priority usage will be given to listed major and minor users with NIH funding, but the instrument also will be available to all UCI researchers.