Cellular and molecular mechanisms of cigarette smoking-induced Paneth cell abnormality

吸烟引起潘氏细胞异常的细胞和分子机制

• 批准号: 10192716
• 负责人: Ta-Chiang Liu
• 金额: $ 35.44万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10192716
• 关键词: Abnormal Cell; Acute; Affect; Apoptosis; Autophagocytosis; Caring; Cell Culture System; Cell Death; Cell Differentiation process; Cell Survival; Cell physiology; Cells; Cellular Morphology; Chronic; Clinical; Clinical Trials; Coculture Techniques; Complex; Cost Measures; Crohn' s disease; Data; Defect; Development; Disease; Disease Outcome; Disease remission; Economic Burden; Environmental Risk Factor; Excision; Exposure to; Functional disorder; Genes; Genetic; Genetic Polymorphism; Genetic Predisposition to Disease; Genetic Risk; Goals; Grant; Hematology; Homeostasis; Immune; In Vitro; Infection; Inflammatory; Inflammatory Bowel Diseases; Injury; Intervention; Intestines; Intrinsic factor; Knock-in; Knock-out; Knockout Mice; Knowledge; Lead; Medical; Modeling; Molecular; Molecular Target; Morphology; Mus; Natural Immunity; Operative Surgical Procedures; Organoids; Outcome; Paneth Cells; Pathology; Pathway interactions; Patients; Pattern; Pharmacology; Phase; Phenotype; Prevalence; Process; Public Health; Recurrence; Research; Risk Factors; Role; Secretory Cell; Signal Pathway; Signal Transduction; Small Intestines; Smoke; Smoker; Smoking; Stimulus; Susceptibility Gene; System; Testing; Therapeutic Intervention; Therapy trial; Variant; Wild Type Mouse; Work; cell type; cellular targeting; chemokine; cigarette smoke; cigarette smoking; combinatorial; cytokine; endoplasmic reticulum stress; experimental study; gene environment interaction; in vivo; inflammatory disease of the intestine; inhibition of autophagy; innovation; insight; intestinal injury; macrophage; mouse model; new therapeutic target; novel; novel therapeutics; pathogen; personalized medicine; programs; response; smoking cessation; stem cells; therapeutic development; therapeutic target; therapy design; therapy development; treatment strategy

ABSTRACT One of the challenges for the management of Crohn’s disease (CD; a chronic intestine inflammatory disorder) is to develop more efficient and personalized treatment strategies. A major reason why CD is difficult to treat is because the disease is induced by both genetic susceptibility and environmental factors. Understanding how CD-relevant gene-environment interaction affects disease outcome will inform the development of therapeutic strategies. We showed that the morphologic phenotype and function of small intestinal Paneth cells are modifiable by integrated effects from both host genetics and known CD environmental risk factor, such that CD patients (and corresponding genetic modified mice) who harbor ATG16L1 T300A polymorphism when exposed to cigarette smoking (a key CD risk factor) develop Paneth cell abnormality. However, the cellular and molecular mechanisms of cigarette smoking-induced Paneth cell abnormality are unknown. Our long-term goal is to dissect the cellular and molecular mechanisms of how gene-environment interactions affect the development and outcome of Crohn’s disease. These discoveries will facilitate design of therapy trials for CD. The objective of this grant is to determine how cigarette smoking induces Paneth cell abnormality. The central hypothesis is that both Paneth cell-intrinsic and –extrinsic factors collectively contribute to smoking-induced Paneth cell defects. Our rationale is that identification of the mechanism(s) to restore Paneth cell function will offer new therapeutic opportunities for CD. Our specific aims will test the following hypotheses: (Aim1) Autophagy induction rescues smoking-induced Paneth cell abnormality; (Aim 2) Intestinal macrophages are activated by cigarette smoking, which in turn triggers Paneth cell apoptosis. Upon conclusion, we will understand the role for autophagy and intestinal macrophages in modulating Paneth cell function. This contribution is significant since it will establish autophagy induction as a new intervention strategy for CD patients with Paneth cell abnormality. The proposed research is innovative because we investigate the effect of autophagy signaling pathways on defective Paneth cells, a heretofore-unexamined process. We also use state-of-the-art intestinal stem cell culture system to identify molecular and cellular targets that affect Paneth cell functions. Identifying the mechanisms of how gene-environment interactions regulate a key disease- relevant cellular phenotype will provide insight into other inflammatory disorders.

摘要 克罗恩病(CD；一种慢性肠炎性疾病)治疗面临的挑战之一 是开发更高效和个性化的治疗策略。CD难以治疗的一个主要原因是 因为这种疾病是由遗传易感性和环境因素共同引起的。了解如何 CD相关基因-环境相互作用影响疾病结局将为治疗的发展提供信息 战略。我们发现小肠潘氏细胞的形态、表型和功能是 可通过宿主遗传学和已知Cd环境风险因素的综合影响而改变，使得Cd 暴露时携带ATG16L1 T300A多态的患者(和相应的转基因小鼠) 吸烟(CD的一个关键危险因素)会导致潘氏细胞异常。然而，蜂窝和 吸烟诱导Paneth细胞异常的分子机制尚不清楚。我们的长期目标 是剖析基因-环境相互作用如何影响细胞和分子机制 克罗恩病的发展和转归。这些发现将有助于CD治疗试验的设计。 这项资助的目的是确定吸烟是如何导致潘氏细胞异常的。中环 假设潘氏细胞的内在因素和外在因素共同作用于吸烟 潘氏细胞缺陷。我们的理论基础是，确定(S)恢复潘氏细胞功能的机制将 为CD提供新的治疗机会。我们的具体目标将检验以下假设：(Aim1) 自噬诱导挽救吸烟诱导的潘氏细胞异常；(目标2)肠道巨噬细胞 吸烟会激活细胞，进而触发潘氏细胞的凋亡。在结束时，我们将 了解自噬和肠道巨噬细胞在调节潘氏细胞功能中的作用。这 这一贡献意义重大，因为它将把自噬诱导确立为CD的一种新的干预策略 潘氏细胞异常的患者。拟议的研究是创新的，因为我们调查了 有缺陷的潘氏细胞上的自噬信号通路，这是一个迄今未被研究的过程。我们还使用 最新的肠道干细胞培养系统，以确定影响Paneth的分子和细胞靶点 细胞功能。确定基因-环境相互作用如何调控一种关键疾病的机制- 相关的细胞表型将提供对其他炎症性疾病的洞察。