Mechanisms Controlling Hormone-Mediated Plant Growth in Response to the Environment

激素介导的植物生长响应环境的控制机制

• 批准号: 10199755
• 负责人: JOANNE CHORY
• 金额: $ 40.74万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10199755
• 关键词: Address; Animals; Biological Models; Complex; Conflict (Psychology); Data; Development; Environment; Exhibits; Gene Expression Regulation; Genes; Genetic; Genetic Transcription; Germination; Growth; Growth and Development function; Hormonal; Hormones; Hour; Human; Individual; Knowledge; Life Cycle Stages; Light; Mediating; Metabolism; Morphogenesis; Neuraxis; Organ; Organ Size; Organelles; Organism; Phenotype; Photoreceptors; Plants; Resolution; Route; Seedling; Seeds; Shapes; Signal Pathway; Signal Transduction; Signal Transduction Pathway; Stress; System; Temperature; Time; To specify; Translating; experimental study; genetic regulatory protein; genetic resource; hormonal signals; light intensity; plant growth/development; predictive modeling; programs; response; sensor

PROJECT SUMMARY/ABSTRACT The mechanisms by which organisms alter their growth and development in response to changes in their ambient environment are largely unknown. Plants exhibit an enormous array of phenotypic plasticity because most plant organs do not arise until after the seed germinates, allowing organ size and shape to be optimized to the local environment. Because plants are sessile and photosynthetic, they are especially attuned to their light environment. Light influences every developmental transition, from seed germination to flowering, having particularly dramatic effects on the morphogenesis of seedlings. During this stage of development, light can alter the expression of thousands of genes within a few hours. Light signals do not act autonomously, but are integrated with seasonal/diurnal changes in temperature and intrinsic programs to specify correct spatial and temporal regulation of gene expression, organelle development, and cellular differentiation. The proposed studies aim to answer the following questions: (1) What are the key signaling pathways and mechanisms that translate information from the environment into changes in growth rate or the initiation of flowering? (2) Are there multiple routes to the same response? (3) In the absence of a central nervous system, how do multicellular organisms integrate a multitude of signals that are spatially and temporally separated and often send conflicting messages? (4) Can we develop predictive models of plant growth? These questions will be addressed using three stages of the plant life cycle that are well studied and known to be highly sensitive to environmental input, namely shade-induced activation of growth, light intensity-induced inhibition of growth, and shade-induced early flowering. In these contexts we will ask how a diverse set of transcriptional responses is generated by the activation of a small number of signaling pathways. The resolution of the studies will be increased using genetic sensors for specific hormone signal transduction pathways. These data will provide an in-depth analysis of hormonal responses to combinations of abiotic stresses. The diverse responses that plants exhibit to light and other environmental parameters provide a unique model system for understanding signaling pathways that regulate phenotypic plasticity. The considerable genetic resources available for each individual signaling pathway make these experiments feasible and timely. Our efforts should contribute significantly to knowledge of complex signal transduction networks and our emerging understanding of how they modulate metabolism in a spatial and temporal manner in multiple kingdoms.

项目总结/摘要 生物体改变其生长和发育的机制，以响应其生长和发育的变化。 周围环境基本上是未知的。植物表现出大量的表型可塑性， 大多数植物器官直到种子发芽后才出现，这使得器官的大小和形状得到优化 to the local本地environment环境.因为植物是固着的和光合作用的，它们特别适应于它们的生长。 光环境。光影响每一个发育过渡，从种子萌发到开花， 尤其是对幼苗形态发生的显著影响。在这个发展阶段，光可以 在几小时内改变数千个基因的表达。光信号并不自主地起作用，而是 与温度和内在程序的季节/昼夜变化相结合，以指定正确的空间和 基因表达、细胞器发育和细胞分化的时间调节。 这些研究旨在回答以下问题：（1）哪些是关键的信号通路， 将来自环境的信息转化为增长率变化或启动的机制 开花？(2)是否有多条路径指向同一个响应？(3)在没有中枢神经系统的情况下， 多细胞生物如何整合大量在空间和时间上分离的信号， 经常发出相互矛盾的信息吗(4)我们能建立植物生长的预测模型吗？这些问题将 使用植物生命周期的三个阶段来解决，这些阶段已经得到充分研究，并且已知对 环境输入，即遮荫诱导的生长激活，光照强度诱导的生长抑制， 和阴诱导的早花。在这些背景下，我们将问，一组不同的转录反应如何 是通过激活少量的信号通路产生的。研究的解决方案将是 使用特定激素信号转导途径的遗传传感器增加。这些数据将提供 深入分析激素对非生物胁迫组合的反应。 植物对光照和其他环境参数的不同反应提供了一个独特的模型 了解调节表型可塑性的信号通路的系统。相当大的遗传 可用于每个单独信号传导途径的资源使得这些实验可行且及时。我们 这些努力将大大有助于了解复杂的信号转导网络， 理解它们如何在多个王国中以空间和时间的方式调节新陈代谢。

项目成果

Dynamic calcium signals mediate the feeding response of the carnivorous sundew plant.

• DOI: 10.1073/pnas.2206433119
• 发表时间: 2022-07-26
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1

Crystal structure of Arabidopsis DWARF14-LIKE2 (DLK2) reveals a distinct substrate binding pocket architecture.

• DOI: 10.1002/pld3.446
• 发表时间: 2022-09
• 期刊: Plant direct
• 影响因子: 3

PIF7 is a master regulator of thermomorphogenesis in shade.

• DOI: 10.1038/s41467-022-32585-6
• 发表时间: 2022-08-29
• 期刊: Nature communications
• 影响因子: 16.6

Stretch-activated ion channels identified in the touch-sensitive structures of carnivorous Droseraceae plants.

• DOI: 10.7554/elife.64250
• 发表时间: 2021-03-16
• 期刊: eLife
• 影响因子: 7.7
• 作者: Procko C;Murthy S;Keenan WT;Mousavi SAR;Dabi T;Coombs A;Procko E;Baird L;Patapoutian A;Chory J
• 通讯作者: Chory J

A Divergent Clade KAI2 Protein in the Root Parasitic Plant Orobanche minor Is a Highly Sensitive Strigolactone Receptor and Is Involved in the Perception of Sesquiterpene Lactones.

根寄生植物小列当中的分化分支 KAI2 蛋白是一种高度敏感的独脚金内酯受体，参与倍半萜内酯的感知。

• DOI: 10.1093/pcp/pcad026
• 发表时间: 2023
• 期刊: Plant & cell physiology
• 影响因子: 4.9
• 作者: Takei,Saori;Uchiyama,Yuta;Bürger,Marco;Suzuki,Taiki;Okabe,Shoma;Chory,Joanne;Seto,Yoshiya
• 通讯作者: Seto,Yoshiya