Transcriptional Regulation in Early Flower Development
早期花发育中的转录调控
基本信息
- 批准号:6520458
- 负责人:
- 金额:$ 35.85万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2001
- 资助国家:美国
- 起止时间:2001-04-01 至 2005-03-31
- 项目状态:已结题
- 来源:
- 关键词:Arabidopsis angiosperms cell cell interaction gene expression genetic mapping genetic regulation genetic regulatory element genetic transcription genetically modified plants high throughput technology homeobox genes intermolecular interaction laboratory rat microarray technology molecular genetics molecular site nucleic acid sequence plant genetics plant growth /development plant morphology plant proteins protein localization protein structure function site directed mutagenesis transcription factor
项目摘要
DESCRIPTION (Applicant's Description): The long-term objective of this proposal
is to understand how transcriptional programs underlying organ-specific
patterns of development are initiated. The experimental system is floral
patterning in the model plant Arabidopsis, which is particularly suited for
genetic and molecular studies because of its short generation time, small
genome, and facile DNA-mediated transformation. The specific problem to be
investigated is how the LEAFY transcription factor interacts with other factors
to regulate flower-specific gene expression. By comparing the processes of
pattern formation in animals and plants -- which evolved multicellularity
independently -- universal principles underlying these processes can be
uncovered. The health relevance of such knowledge relates to birth defects
resulting from aberrant pattern formation caused by environmental effects, and
to genetic disorders disrupting genes encoding developmental regulators.
LEAFY, which is the earliest known transcription factor to be specifically
activated in floral primordia, is both necessary and sufficient for converting
vegetative shoots into flowers. A unique feature of LEAFY compared to animal
transcription factors is that it can be exported to adjacent cells, where it
can activate direct target genes. One class of direct target s, homeotic genes
that control floral organ fate, has been previously identified, and includes
the AGAMOUS gene. However, other targets besides homeotic genes must be
required for flower formation, since flowers are distinguished from shoots not
only by differences in organ identity but also in organ arrangement.
Experiments are proposed (1) to study AGAMOUS regulation as a proxy for
transcriptional control during plant development; (2) to reveal the global
expression program induced by LEAFY with the goal of uncovering new classes of
target genes; and (3) to identify the cis- and trans-requirements for LEAFY
movement with the goal of elucidating this unique mode of cell-cell
communication. The proposed experiments will advance our understanding of how
widely expressed, early acting genes participate in the regulation of
later-acting genes that are expressed only in restricted regions of an emerging
organ primordium. The experiments will also reveal how different stem cells
communicate to effect coordinated development of the flower.
描述(申请人的描述):该提案的长期目标
是了解器官特异性的转录程序
发展模式已启动。实验系统为花卉
模式植物拟南芥中的图案化,特别适合
遗传和分子研究因其世代时间短、规模小
基因组和简单的 DNA 介导的转化。具体问题要
研究的是 LEAFY 转录因子如何与其他因子相互作用
调节花特异性基因表达。通过比较流程
动物和植物的模式形成——进化出多细胞性
独立地——这些过程背后的普遍原则可以是
裸露。这些知识的健康相关性与出生缺陷有关
由于环境影响导致异常图案形成,以及
破坏编码发育调节因子的基因的遗传疾病。
LEAFY,这是已知最早的特异性转录因子
在花原基中被激活,对于转化来说既是必要的也是充分的
营养芽变成花。 LEAFY 与动物相比的独特之处
转录因子的特点是它可以输出到邻近的细胞,在那里它
可以激活直接靶基因。一类直接靶标,同源异型基因
控制花器官命运的基因先前已被识别,包括
AGAMOUS 基因。然而,除了同源异型基因之外,还必须考虑其他靶标
花形成所必需的,因为花与芽不同
不仅取决于器官身份的差异,还取决于器官排列的差异。
建议进行实验 (1) 研究 AGAMOUS 调节作为代理
植物发育过程中的转录控制; (2)揭示全局
LEAFY 诱导的表达程序,目的是发现新的类别
靶基因; (3) 确定 LEAFY 的顺式和反式要求
旨在阐明这种独特的细胞-细胞模式的运动
沟通。所提出的实验将加深我们对如何
广泛表达的早期作用基因参与调节
后期作用基因仅在新兴基因的有限区域表达
器官原基。实验还将揭示不同的干细胞如何
沟通以实现花卉的协调发展。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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JOANNE CHORY其他文献
JOANNE CHORY的其他文献
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{{ truncateString('JOANNE CHORY', 18)}}的其他基金
Mechanisms Controlling Hormone-Mediated Plant Growth in Response to the Environment
激素介导的植物生长响应环境的控制机制
- 批准号:
10199755 - 财政年份:2017
- 资助金额:
$ 35.85万 - 项目类别:
Molecular Mechanisms of Steroid Hormone Perception at the Cell Surface
细胞表面类固醇激素感知的分子机制
- 批准号:
8320149 - 财政年份:2011
- 资助金额:
$ 35.85万 - 项目类别:
Molecular Mechanisms of Steroid Hormone Perception at the Cell Surface
细胞表面类固醇激素感知的分子机制
- 批准号:
8186473 - 财政年份:2011
- 资助金额:
$ 35.85万 - 项目类别:
Molecular Mechanisms of Steroid Hormone Perception at the Cell Surface
细胞表面类固醇激素感知的分子机制
- 批准号:
8474788 - 财政年份:2011
- 资助金额:
$ 35.85万 - 项目类别:
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