Integrative analysis of the oral microbiome of American Indian Infants and the impact of a childhood obesity-prevention home visiting program on early childhood caries

美洲印第安婴儿口腔微生物组的综合分析以及儿童肥胖预防家访计划对儿童早期龋齿的影响

• 批准号: 10201570
• 负责人: Catherine Gayle Sutcliffe
• 金额: $ 16.41万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10201570
• 关键词: 1 year old; 16S ribosomal RNA sequencing; 2 year old; Acids; Address; Adult; Affect; Age; Age-Months; American Indians; Child; Childhood; Clinical Trials; Collaborations; Communities; Consumption; Dental Enamel; Dental caries; Development; Diet; Dietary Sugars; Dietary intake; Education; Enrollment; Environment; Epidemiologist; Family; Food; Future; Goals; Health; Health Care Costs; Health Service Area; High Prevalence; Home visitation; Infant; Infection; Intake; Intervention; Investigation; Knowledge; Metabolic; Metagenomics; Mothers; Navajo; Nutritional; Oral; Oral health; Outcome; Pain; Pathogenicity; Pattern; Performance; Prevalence; Prevention program; Probiotics; Production; Race; Randomized Controlled Trials; Research; Research Personnel; Resources; Risk; Saliva; Sampling; Schools; Speech Delay; Speech Development; Testing; Therapeutic Intervention; Time; Tribes; United States; United States Indian Health Service; aged; bacterial community; base; carbohydrate metabolism; cariogenic bacteria; demineralization; drinking water; early childhood; experience; feeding; health disparity; high risk; intervention program; liquid chromatography mass spectrometry; metabolic profile; metabolome; metabolomics; metatranscriptomics; microbial; microbial community; microbiome; microbiome composition; microbiota; microbiota profiles; next generation; obesity in children; obesity prevention; oral bacteria; oral microbial community; oral microbiome; prebiotics; programs; self esteem; sugar; sweetened beverage

PROJECT SUMMARY/ABSTRACT Early childhood caries (ECC) has devastating and long-lasting health consequences. These include higher healthcare costs, pain, infection, lower self-esteem, delayed speech development, poor performance in school, and subsequent poor oral and overall health outcomes as adults. The prevalence of ECC in children of American Indian (AI) families is considerably higher compared to all races in the United States, with ECC occurrence as early as one year of age. ECC and the closely related health disparity early childhood obesity (ECO), are critical for young AI children, especially in the Navajo community that has limited access to nutritious food and clean, safe drinking water. Diet is a crucial contributing factor to ECC and ECO, where high dietary sugar intake and early initiation of sugar-sweetened beverages (SSBs) before six months of age contribute significantly to both conditions in Navajo infants. Frequent sugar consumption drives the selection of cariogenic bacteria and associated metabolic activities within the oral microbiota that ultimately results in caries development. Acquisition and proliferation of cariogenic bacteria before two years of age have been associated with an increased risk of caries. However, the development of the oral microbiome and the impact of the sugar intake reduction on the pattern of microbiota in high-risk Navajo infants remain largely unknown. Recently, an ECO prevention program, called Family Spirit Nurture, was implemented in the Navajo Shiprock community through a randomized controlled trial. The intervention focused on infant feeding practices and SSB intake reduction. The trial enrolled mother-infant dyads and collected longitudinal oral samples from infants aged 3 to 12 months, maternal and child confounding factors, and ECC status at 1 year of age. This trial provided an unparalleled opportunity for the longitudinal investigation of Navajo infants at an early age with two important specific aims: 1) evaluate the developing oral microbiome of Navajo infants in relation to maternal and child confounding factors and the potential impact of the sugar reduction intervention on the oral microbiome profiles and caries outcomes and 2) investigate the metabolome of Navajo infants’ oral microbial communities in correlation with ECC outcomes at 12 months of age. We hypothesize that 1) the sugar reduction intervention may influence the oral microbiome composition toward non-cariogenic species and reduce ECC outcome at 12 months of age, 2) there would be developmental changes in the oral microbiome of caries-free Navajo infants and pathogenic shifts in the oral microbiome pattern for those infants who eventually develop caries, 3) Microbial communities of the caries- affected group at 12 months of age may have a higher level of carbohydrate metabolism-related metabolites compared to the caries-free group, and 4) there exists caries-related metabolite signatures of Navajo infants. The goal of this study is to evaluate if the oral microbial and metabolic profiles of Navajo infants could be used to predict ECC and to develop a therapeutic intervention through future research plans.

项目摘要/摘要 幼儿汽车（ECC）具有毁灭性和持久的健康后果。这些包括 更高的医疗保健成本，疼痛，感染，自尊心较低，语音发展延迟，表现不佳 成年后，学校，随后的口腔贫困和整体健康状况。 ECC在儿童的患病率 与美国的所有种族相比 早在一岁时就发生。 ECC和密切相关的健康差异幼儿肥胖 （ECO），对于年轻的AI儿童至关重要，尤其是在纳瓦霍社区的机会有限的纳瓦霍人社区中 营养食品和清洁，安全的饮用水。饮食是对ECC和ECO的至关重要因素，那里很高 六个月大的饮食糖摄入量和早期开始糖甜卧室（SSB） 对纳瓦霍婴儿的两种疾病都有显着贡献。频繁的糖消耗驱动选择 口服微生物群中的致癌细菌和相关的代谢活性，最终导致龋齿 发展。两岁之前的核原细菌的获取和增殖与 汽车风险增加。但是，口服微生物组的发展和糖的影响 高危纳瓦霍婴儿的菌群模式的摄入量降低仍然未知。 最近，纳瓦霍人实施了一项名为“家庭精神养育”的生态预防计划 通过随机对照试验，船岩社区。干预措施的重点是婴儿喂养实践 和SSB摄入量减少。该试验招募了母亲的二元组，并从 3至12个月的婴儿，母亲和儿童混淆因素以及1岁以上的ECC状况。这 试验为对纳瓦霍婴儿的纵向调查提供了无与伦比的机会 两个重要的具体目的：1）评估纳瓦霍婴儿的口服微生物组 母婴混淆因素以及减少糖干预对口腔的潜在影响 微生物组轮廓和汽车结果以及2）研究纳瓦霍婴儿口服微生物的代谢组 与12个月大的ECC结局相关的社区。 我们假设1）减少糖干预可能会影响口服微生物组成分 迈向非钙化物种并减少12个月大时的ECC结果，2） 口服纳瓦霍婴儿的口服微生物组的发育变化和口腔中的致病变化 有时开发汽车的婴儿的微生物组模式，3）汽车的微生物群落 - 12个月大的患病组可能具有较高的碳水解代谢相关代谢物 与无龋齿组相比，4）存在纳瓦霍婴儿的龋齿相关的代谢物特征。 这项研究的目的是评估是否可以使用纳瓦霍婴儿的口腔微生物和代谢特征 通过未来的研究计划来预测ECC并开发治疗性干预措施。