Organ Specific Project - Kidney

器官特定项目 - 肾脏

• 批准号: 10201589
• 负责人: RAYMOND C. HARRIS
• 金额: $ 115.85万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-24 至 2022-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10201589
• 关键词: 3-Dimensional; APOL1 gene; Address; Affect; African American; American; Anatomy; Antibodies; Atlases; Biological Assay; Biology; Blood capillaries; Cell Separation; Cells; Characteristics; Chronic Kidney Failure; Clinical; Collection; Cytometry; Data; Development; Disease susceptibility; Dissociation; Elements; Emerging Technologies; Ensure; Epidemiology; Excision; Extracellular Matrix; Filtration; Fluorescence; Gene Expression Profiling; Genetic; Goals; Health; Heterogeneity; Histologic; Homeostasis; Human; Human BioMolecular Atlas Program; Image; Imaging technology; Immunohistochemistry; Incidence; Individual; Investigation; Kidney; Kidney Diseases; Knowledge; Link; Lipids; Lymphatic; Magnetic Resonance Imaging; Mass Spectrum Analysis; Methods; Mission; Molecular; Molecular Analysis; Molecular Profiling; Morphology; Multimodal Imaging; Nephrons; Operative Surgical Procedures; Organ; Personal Satisfaction; Preparation; Property; Proteins; Resolution; Risk; Sampling; Sodium Chloride; Source; Specimen; Standardization; Structure; Tissue Banks; Tissues; Tubular formation; Universities; Variant; Water; assay development; base; biobank; blood pressure regulation; cell type; data management; experimental study; extracellular; genetic association; genetic variant; human tissue; imaging modality; insight; interstitial; kidney cell; male; microCT; molecular imaging; multimodality; programs; racial disparity; scale up; sex; surgery material; three dimensional structure

Project Summary The goal of the Kidney OSP is to address the limitation in current knowledge of kidney biology by developing an ultra-high content mass spectrometry (MS)-based 3-dimensional (3-D) imaging pipeline to characterize the molecular signatures (lipids, metabolites and proteins) of different cell types and extracellular matrix components at high resolution in the intact human kidney. The imaging data acquired by this unique platform will be aligned and fused with the 3-D structure of the human kidney obtained by coordinate-based alignment with MRI, CT and histochemical based images. This technological pipeline will be developed to be compatible with other molecular interrogation pipelines generated through HuBMAP to create a comprehensive multidimensional 3-D molecular atlas of the normal human kidney.

项目总结