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New computational, transcriptional, and genome editing approaches to the biology of inflammatory bowel disease

研究炎症性肠病生物学的新计算、转录和基因组编辑方法

基本信息

批准号：
10200800
负责人：
GREGORY C GIBSON
金额：
$ 17.27万
依托单位：
GEORGIA INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-19 至 2023-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10200800
关键词：
Address Affect African American Alleles American Ancillary Study Architecture Area Award Bar Codes Biological Assay Biology Biopsy Blood CRISPR/Cas technology Categories Cells Clinical Clustered Regularly Interspaced Short Palindromic Repeats Complement Complex Crohn&apos s disease Cytokine Signaling DNA Data Data Coordinating Center Data Set Development Disease Disease Progression Drug Delivery Systems Epithelial Cells Ethnic Origin European Extracellular Matrix Foundations Functional disorder Gene Expression Genes Genetic Genetic Diseases Genetic Engineering Genetic Polymorphism Genetic Risk Genetic Transcription Genomic approach Genomics Guide RNA Human Immune Individual Inflammatory Bowel Diseases Intestines Knock-out Laboratories Location Mediating Messenger RNA Mitochondria Molecular National Institute of Diabetes and Digestive and Kidney Diseases Organ Culture Techniques Organoids Outcome Pathogenesis Pathology Patient-Focused Outcomes Patients Pharmacologic Substance Pharmacology Play Protocols documentation Quantitative Genetics Reagent Research Research Personnel Resources Risk Risk Assessment Role Side Signal Transduction Site Source Susceptibility Gene System Transcript Translating Ulcerative Colitis aptamer base causal variant cell type gain of function gastrointestinal epithelium gene discovery gene expression variation gene therapy genetic analysis genetic approach genetic manipulation genome editing genome sequencing genome wide association study improved in silico in vivo intestinal epithelium knockout gene lipid nanoparticle mRNA delivery member nanoparticle nanoparticle delivery novel novel strategies patient response rectal response risk variant single-cell RNA sequencing therapeutic evaluation transcriptome transcriptomics treatment response whole genome

项目摘要

Project Summary Over the past decade, the NIDDK IBDGC (Inflammatory Bowel Disease Genetics Consortium) has generated extraordinary datasets in support of genetic analysis of the onset, progression, and therapeutic response to Crohn's disease and ulcerative colitis. This Ancillary project will complement ongoing IBDGC research by providing parallel statistical genetic analyses focused on transcriptomics, while also developing a novel strategy for genetic manipulation of patient-derived epithelial cells. There are four major biomedical genomics focus areas addressed by the research, namely fine mapping of loci influencing inflammatory bowel disease, elucidation of the cell and molecular function of causal genes, understanding how polymorphism influences pathology, and translating quantitative genetic discoveries into clinical outcomes. Specifically, integrative genomics expertise will be used to refine the credible intervals responsible for complex association signals at individual loci, enhance transcriptional risk scores (TRS) that have recently been shown to provide much greater prediction of disease and progression than genetic risk scores, and explore the potential of in silico predicted transcriptome-wide association studies in the context of IBD. These studies will utilize the IBDGC datasets through collaborative arrangements mediated by data coordinating center. In addition, proof of principle for the use of lipid nanoparticles as an efficient and specific delivery system for genome editing and/or pharmaceutical delivery to targeted cell types in gut- derived organoids will be demonstrated. Single cell RNA-Seq will be used to partition variability in gut epithelial gene expression in the half dozen most common organoid cell types into contributions of the ethnicity, location of the biopsy, type of disease, and source laboratory. This data will serve as a foundation for evaluating the effects of a half dozen gene knock-outs across cell types using the lipid nanoparticle delivery system. All analyses and reagents will be made available to consortium members as expected for collaborative IBDGC research.

项目摘要在过去的十年中，NIDDK IBDGC（炎症性肠病遗传学联盟）生成了非凡的数据集，以支持对发病、进展和治疗的遗传分析。对克罗恩病和溃疡性结肠炎的反应。该辅助项目将补充正在进行的IBDGC 研究通过提供平行的统计遗传分析集中在转录组学，同时也发展一种新的策略，用于遗传操作的患者来源的上皮细胞。有四大生物医学基因组学的重点领域解决的研究，即基因座的精细映射影响炎症性肠病，阐明致病基因的细胞和分子功能，理解多态性如何影响病理学，并将定量遗传学发现转化为临床结果。具体而言，将使用综合基因组学专业知识来完善可信区间负责单个基因座的复杂关联信号，增强转录风险评分（TRS），最近显示，与遗传风险相比，评分，并探索在计算机模拟预测的转录组范围内的关联研究的潜力， IBD。这些研究将通过以数据为媒介的合作安排利用IBDGC数据集协调中心。此外，使用脂质纳米颗粒作为一种有效的和有效的治疗方法的原理证明，用于基因组编辑和/或药物递送到肠内靶向细胞类型的特异性递送系统，将展示衍生的类器官。单细胞RNA-Seq将用于划分肠道中的变异性上皮基因表达在半打最常见的类器官细胞类型的贡献，种族、活检位置、疾病类型和源实验室。这些数据将作为基础用于评估使用脂质纳米颗粒的六种基因敲除对细胞类型的影响，输送系统。所有分析和试剂将按预期提供给联合体成员， IBDGC合作研究。