The relationship between socioeconomic status and poverty and mRNA expression in immune cells

社会经济地位与贫困与免疫细胞mRNA表达的关系

• 批准号: 10372118
• 负责人: Douglas F. Dluzen
• 金额: $ 22.05万
• 依托单位: MORGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-31 至 2024-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10372118
• 关键词: African American population; African ancestry; Atherosclerosis; Baltimore; Bioinformatics; Biological; Biology; C-reactive protein; Candidate Disease Gene; Cardiovascular Diseases; Cardiovascular system; Caucasians; Cell Culture Techniques; Cells; Cholesterol; Cities; Cross-Sectional Studies; Data; Development; Disease; Disease Outcome; Disease Progression; Endothelial Cells; Environmental Risk Factor; Exhibits; Female; Gender; Gene Expression; Gene Expression Profile; Genes; Genetic; Goals; Health Disparities Research; High Density Lipoproteins; Hypertension; Immune; Immune System Diseases; Immune system; In Vitro; Individual; Inflammatory; Inflammatory Response; Inflammatory Response Pathway; Investigation; Knowledge; Laboratories; Link; Longevity; Longitudinal cohort; Low-Density Lipoproteins; Messenger RNA; Minority Groups; Modeling; Neighborhoods; Ontology; Participant; Pathology; Pathway interactions; Pattern; Peripheral Blood Mononuclear Cell; Poverty; Predisposition; Prevalence; Production; Psychosocial Stress; Race; Research; Risk; Serum; Signal Pathway; Socioeconomic Status; Stress; Stroke; Subgroup; Therapeutic Intervention; Tissue-Specific Gene Expression; Tissues; Underrepresented Minority; Work; chemokine; cohort; comparative; cytokine; differential expression; follow-up; gene function; gene network; health care availability; health disparity; healthy aging; innovation; lifestyle factors; low socioeconomic status; mRNA Expression; male; minority communities; novel; novel strategies; novel therapeutics; nutrition; poor communities; potential biomarker; programs; social factors; social health determinants; social influence; transcriptome sequencing; urban health disparities; validation studies

Project Summary/Abstract: The causes of health disparities in inflammatory diseases, including cardiovascular diseases (CVDs) such as hypertension, atherosclerosis, and stroke, are multifactorial and include, but are not limited to, socioeconomic status (SES) and poverty, lifestyle factors, psychosocial stress, genetics, and poor access to health care. However, how environmental factors influence the predisposition to- and pathology of- these conditions remain predominantly unexplored, particularly in minority communities at-risk for these conditions. Our long-term goal is to understand how poverty status influences gene expression in cells immune system and elucidate how this contributes to the biological mechanisms that influence the prevalence of related health disparities. Previous work in our laboratory and elsewhere has identified differential gene expression patterns in immune cells isolated from minorities populations, including African Americans, that are influenced by social factors, including poverty. Importantly, there have been no prior studies that examine the intersection of SES, gender, and race as factors that influence differential gene expression in the immune system. Our objective is to elucidate the effect of SES on gene expression in peripheral blood mononuclear cells and identify the longitudinal impact on inflammatory gene expression due to living in poverty. Our hypothesis is that living in low SES and poverty is associated with differential gene expression profiles within inflammatory-related pathways. This proposal will significantly contribute to our understanding of how the environmental stress of poverty influences the expression of genes in tissues related to the cardiovascular system. Additionally, this proposal will examine gene expression in both males and females of Caucasian and African ancestry in order to further understand the biology of health disparities. The proposed research is innovative because this approach will examine gene expression in a longitudinal cohort and combine both bioinformatic and predictive analysis with rigorous validation studies.

项目概要/摘要： 炎症性疾病（包括心血管疾病（CVD），如 高血压、动脉粥样硬化和中风是多因素，包括但不限于社会经济因素 社会经济地位（SES）和贫困，生活方式因素，心理社会压力，遗传学和难以获得医疗保健。 然而，环境因素如何影响这些疾病的易感性和病理学仍然存在 主要是未开发的，特别是在面临这些条件风险的少数民族社区。我们的长期目标 是了解贫困状况如何影响细胞免疫系统中的基因表达，并阐明这是如何 有助于影响相关健康差异普遍性的生物机制。先前 我们实验室和其他地方的工作已经确定了分离的免疫细胞中的差异基因表达模式 包括非裔美国人在内的少数民族人口受到社会因素的影响，包括贫困。 重要的是，之前没有研究将SES、性别和种族作为因素进行研究 影响免疫系统中基因表达的差异。我们的目标是阐明SES的影响 对外周血单个核细胞基因表达的影响，并确定对炎症的纵向影响。 因为生活在贫困中，基因表达。我们的假设是，生活在低社会经济地位和贫困与 炎症相关通路中的差异基因表达谱。该提案将大大 有助于我们理解贫困的环境压力如何影响基因的表达 与心血管系统相关的组织中。此外，这项建议将检查基因表达在这两个 高加索和非洲血统的男性和女性，以进一步了解健康的生物学 差距。这项研究是创新的，因为这种方法将在一个特定的基因表达水平上检查基因表达。 纵向队列和联合收割机将生物信息学和预测分析与严格的验证研究相结合。