Myosin 18 and its role in skeletal muscle

肌球蛋白 18 及其在骨骼肌中的作用

• 批准号: 10378608
• 负责人: H Lee Sweeney
• 金额: $ 40.87万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10378608
• 关键词: ATP Hydrolysis; ATP phosphohydrolase; Actins; Address; Binding; Biochemical; Biological; Biological Assay; Cell Culture Techniques; Cell physiology; Cells; Data; Development; Elements; Filament; Generations; Genes; Goals; Human; Hydrolysis; Investigation; Kinetics; Length; Light; Maintenance; Mass Spectrum Analysis; Modeling; Molecular Conformation; Motor; Motor Activity; Muscle; Muscle Cells; Muscle Fibers; Mutagenesis; Mutation; Myocardium; Myopathy; Myosin ATPase; Myosin Type II; Nucleotides; Phosphorylation; Phosphorylation Site; Play; Preparation; Process; Protein Isoforms; Proteins; Publishing; RNA Splicing; Regulation; Resolution; Roentgen Rays; Role; Sarcomeres; Scaffolding Protein; Skeletal Muscle; Striated Muscles; Structure; Surface; Tissues; Transcript; Vertebrates; base; cell motility; cell type; congenital myopathy; design; dimer; experimental study; insight; non-muscle myosin; novel; recruit

Myosin 18 and its role in skeletal muscle: H. Lee Sweeney, P.I. Abstract: This project will investigate the structure and function of an unconventional myosin, myosin XVIII (Myo18). One of the two forms Myo18 expressed in vertebrates, Myo18B, is critical for the generation of sarcomeres in striated muscle. The other form, Myo18A is found in all cells, including muscle, where it may function in other critical cellular processes that require Myo2 filament recruitment and organization, including the early stages of sarcomere formation in striated muscle. Mutations that result in the loss of Myo18B result in myopathies in humans. While Myo18 has been characterized as a non-motor, scaffolding protein, that aids in the assembly of Myo2 filaments, we postulate that it is in fact an active motor protein subject to a novel form of regulation. Only by understanding the design and function of this motor, and using those insights to probe sarcomere formation in muscle cells, will we begin to understand the roles that Myo18 plays in striated muscle and ultimately in other tissues.

肌球蛋白 18 及其在骨骼肌中的作用：H. Lee Sweeney, P.I. 抽象的： 该项目将研究非常规肌球蛋白 XVIII (Myo18) 的结构和功能。一 Myo18 在脊椎动物中表达的两种形式中的 Myo18B 对于肌节的生成至关重要 横纹肌。另一种形式，Myo18A 存在于所有细胞中，包括肌肉，它可能在其他细胞中发挥作用。 需要 Myo2 丝募集和组织的关键细胞过程，包括早期阶段 横纹肌肌节的形成。导致 Myo18B 缺失的突变会导致肌病 人类。虽然 Myo18 被描述为一种非运动支架蛋白，但它有助于组装 Myo2 丝，我们假设它实际上是一种受到新型调节形式的活性运动蛋白。仅有的 通过了解该马达的设计和功能，并利用这些见解来探测肌节的形成 在肌肉细胞中，我们是否会开始了解 Myo18 在横纹肌中以及最终在 其他组织。