Myo10-Driven Filopodia in Skeletal Muscle

骨骼肌中 Myo10 驱动的丝状伪足

• 批准号: 10634534
• 负责人: H Lee Sweeney
• 金额: $ 37.6万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10634534
• 关键词: Actins; Acute; Age; Blood Vessels; Breathing; Cell Culture Techniques; Cell Nucleus; Cell fusion; Cells; Characteristics; Chimeric Proteins; Chronic; Data; Defect; Development; Direct Lytic Factors; Disease; Duchenne muscular dystrophy; Female; Fiber; Filopodia; Freezing; Genetic; Growth; Human; In Vitro; Injury; Knock-out; Laboratories; Locomotion; Mammals; Modeling; Molecular Motors; Motor; Movement; Mus; Muscle; Muscle Cells; Muscle Development; Muscle Fibers; Muscle satellite cell; Myoblasts; Myopathy; Myosin ATPase; Natural regeneration; Nature; Neoplasm Metastasis; Phenotype; Physiological; Play; Probability; Process; Proteins; Regulation; Role; Sarcomeres; Skeletal Muscle; Skeleton; Tissues; experimental study; in vivo; in vivo regeneration; insight; male; muscle regeneration; neural; postnatal; regeneration following injury; repaired; satellite cell; skeletal muscle growth; stem cells; transmission process

Myo10-Driven Filopodia in Skeletal Muscle: H. Lee Sweeney, P.I. Abstract: This project will investigate the role of an unconventional myosin, myosin X (Myo10), and the filopodia that it generates in myoblasts and nascent myotubles. We postulate that it is only present in muscle cells/satellite cells that are actively in the process of fusing with each other, which would be during muscle growth and regeneration. We further postulate that Myo10 forms filopodia and carries components of the fusion machinery to the tips of those filipodia, greatly increasing the efficiency and probability of cell fusion. We will examine the consequences of loss of Myo10 and its interactions in myogenic cells in the following aims: 1) Delineate the role of myosin X (Myo10) involvement in myoblast fusion and identify regulatory mechanisms of protein targeting and activation in vitro; 2) Evaluate the requirement of myosin X (Myo10) for muscle regeneration; and, 3) Investigate Myosin X (Myo10) as a modifier of chronic muscle disease. These experiments will uncover previously unstudied aspects of the fusion process for skeletal muscle growth and regeneration, and may delineate new modifiers/targets in disease states of skeletal muscle that require rapid muscle regeneration using satellite cells.

骨骼肌肌10驱动丝状足：H. Lee Sweeney， P.I.