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Stretchable Hydrogel Bioinks-Enabled Microfluidic Bioprinting of Functional Small-Diameter Blood Vessels

可拉伸水凝胶生物墨水支持功能性小直径血管的微流体生物打印

基本信息

批准号：
10212452
负责人：
Xuanhe Zhao
金额：
$ 46.2万
依托单位：
MASSACHUSETTS INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-07-10 至 2025-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10212452
关键词：
3-Dimensional Address Anastomosis - action Architecture Arteries Back Biocompatible Materials Biological Biology Biomimetics Bioreactors Blood Blood Vessels Blood capillaries Blood flow Caliber Cardiovascular system Cells Chemistry Complex Computer software Elastic Fiber Endothelial Cells Engineering Ensure Evaluation Fiber Fibroblasts Formulation Future Heart Hydrogels In Vitro Instruction Manuals Mechanical Stimulation Mechanics Medicine Methods Microfabrication Microfluidics Modeling Mus Nerve Nutrient Organ Pattern Physiological Play Polymers Polysaccharides Procedures Property Radial Relaxation Reporting Reproducibility Resolution Rest Rodent Model Role Scheme Series Shapes Smooth Muscle Myocytes Stretching Structure System Technology Thick Tissue Engineering Tissues Transplantation Transportation Tunica Adventitia Vascular Graft Vascular Smooth Muscle Veins base biomaterial compatibility bioprinting cell type constriction crosslink digital experience flexibility hemodynamics in vivo in vivo evaluation mechanical properties programs repaired scaffold shear stress

项目摘要

Abstract Blood vessels play a critical role in the circulatory system. The main function of blood vessels is transporting blood from the heart to the rest of the tissues and organs throughout the body and then bringing it back to the heart. The structures of blood vessels are crucial to their physiological functions. The intima consists of endothelial cells, which are intertwined with a polysaccharide intercellular matrix to form the lumen for blood transportation. In straight sections of a blood vessel, endothelial cells (ECs) typically align and elongate in the direction of blood flow. The media is the middle layer in the vessels, where the elastic fibers, polysaccharides, and vascular smooth muscle cells (SMCs) are mainly located. In particular, the circumferentially aligned SMCs in ring-like patterns control the constriction/dilation of the vessels, enabling modulation of hemodynamics. Tissue engineering has provided a promising strategy to repair and replace portions of tissues, where blood vessels are one of the most important yet challenging tissue to engineer. However, engineered blood vessels using conventional strategies based on scaffolds are usually produced using relatively sophisticated microfabrication procedures, and cannot be easily applied to vessels with complex architectures and/or small sizes. In comparison, the recent advances in the three-dimensional (3D) bioprinting technology have provided unprecedented flexibility in engineering blood vessels with high resolution, strong fidelity, and good complexity. Nevertheless, 3D bioprinting of structurally stable and functional vascular tissues has rarely been achieved. To this end, we propose to develop a unique bioprinting strategy, combining the digitally tunable microfluidic hollow fiber bioprinting method and the stretchable hydrogel-based bioink formulations, to generate structurally, mechanically, and functionally biomimetic non-branching macrovascular grafts of various sizes, shapes, and structures to significantly facilitate vascular transplantation.

摘要