Single-Cell Transcriptomic and Epigenetics Core

综述-单细胞遗传学和表观遗传学核心： 单细胞转录组学和表观遗传学（SCTE）核心将支持所有三个项目， 生物信息学和计算生物学（BCB）的核心是产生强大的单细胞转录组学和 小细胞数量表观遗传数据集以及进行DNA测序以解释体内 基于shRNA的功能筛选。SCTE核心将提供用于生成单小区的集中式资源。 RNA测序、组蛋白修饰ChIP-seq、转录因子ChIP-seq和ATAC-seq数据集。 此外，SCTE核心将生成和分析深度DNA测序数据，以确定 在功能筛选的背景下，shRNA在转导的CD 8 + T细胞中的分布。我们设想 使用单个核心将增加实验方法的严谨性和可重复性，增加协同作用， 在调查人员和实验室之间，并能够直接比较所得数据集。一代 这些数据集将使BCB核心项目和单个项目能够实现阐明 分子异质性，转录和表观遗传调控，以及组织驻留CD 8 + T细胞的功能 在急性和慢性感染的情况下。 !

SUMMARY-SINGLE CELL TRANSCRIPTOMICS AND EPIGENETICS CORE: The Single-Cell Transcriptomics and Epigenetics (SCTE) Core will support all three Projects and the Bioinformatics and Computational Biology (BCB) Core in generating robust single-cell transcriptomic and small-cell-number epigenetic datasets as well as performing DNA-sequencing for interpretation of in vivo shRNA-based functional screens. The SCTE Core will provide a centralized resource for generating single-cell RNA-sequencing, histone modification ChIP-seq, transcription factor ChIP-seq, and ATAC-seq datasets. Additionally, the SCTE Core will generate and analyze deep DNA-sequencing data to determine the distribution of shRNAs among transduced CD8+ T cells in the context of the functional screens. We envision that use of a single Core will increase rigor and reproducibility in experimental methodology, increase synergy among investigators and laboratories, and enable direct comparison of the resulting datasets. Generation of these datasets will enable the BCB Core and individual Projects to achieve the overall goal of elucidating the molecular heterogeneity, transcriptional and epigenetic regulation, and function of tissue-resident CD8+ T cells in the context of acute and chronic infection. !