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Single-Cell Transcriptomic and Epigenetics Core

单细胞转录组学和表观遗传学核心

基本信息

批准号：
10453788
负责人：
Eugene Wei-Ming Yeo
金额：
$ 29.89万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-07-17 至 2024-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10453788
关键词：
ATAC-seq Acute Animal Model Bioinformatics CD8-Positive T-Lymphocytes Candidate Disease Gene Cell Line Cells ChIP-seq Chronic Common Core Computational Biology Core Facility DNA Methylation DNA analysis DNA sequencing Data Data Analyses Data Set Databases Deposition Doctor of Philosophy Epigenetic Process Exhibits Experimental Designs Gene Expression Profiling Generations Genes Genetic Genetic Screening Genetic Transcription Genome Genomic medicine Genomics Goals Growth Heterogeneity Individual Institutes Knock-out Laboratories Link Measures Memory Methodology Modeling Molecular Molecular Medicine Pathway interactions Patients Population Process Proteins Quality Control RNA Processing Regulatory Element Reproducibility Research Activity Research Personnel Research Project Grants Resources Sampling Scientist Standardization Supervision T cell differentiation T memory cell T-Lymphocyte Testing Time Tissues Transcriptional Regulation Validation Virus Diseases acute infection base chronic infection cost effective data quality effector T cell epigenetic regulation exhaust exhaustion genome-wide histone modification in vivo innovation insight member nervous system disorder novel professor single cell analysis single-cell RNA sequencing small hairpin RNA stem cells synergism transcription factor transcriptome transcriptomics

项目摘要

SUMMARY-SINGLE CELL TRANSCRIPTOMICS AND EPIGENETICS CORE: The Single-Cell Transcriptomics and Epigenetics (SCTE) Core will support all three Projects and the Bioinformatics and Computational Biology (BCB) Core in generating robust single-cell transcriptomic and small-cell-number epigenetic datasets as well as performing DNA-sequencing for interpretation of in vivo shRNA-based functional screens. The SCTE Core will provide a centralized resource for generating single-cell RNA-sequencing, histone modification ChIP-seq, transcription factor ChIP-seq, and ATAC-seq datasets. Additionally, the SCTE Core will generate and analyze deep DNA-sequencing data to determine the distribution of shRNAs among transduced CD8+ T cells in the context of the functional screens. We envision that use of a single Core will increase rigor and reproducibility in experimental methodology, increase synergy among investigators and laboratories, and enable direct comparison of the resulting datasets. Generation of these datasets will enable the BCB Core and individual Projects to achieve the overall goal of elucidating the molecular heterogeneity, transcriptional and epigenetic regulation, and function of tissue-resident CD8+ T cells in the context of acute and chronic infection. !

摘要-单细胞转录学和表观遗传学核心：单细胞转录和表观遗传学(SCTE)核心将支持所有三个项目和生物信息学和计算生物学(BCB)核心在产生健壮的单细胞转录和小细胞数量表观遗传学数据集以及进行DNA测序以解释体内基于shRNA的功能筛选。SCTE核心将提供用于产生单小区的集中资源 RNA测序、组蛋白修饰芯片SEQ、转录因子芯片SEQ和ATAC-SEQ数据集。此外，SCTE核心将生成和分析深度DNA测序数据，以确定在功能筛选的背景下shRNA在转导的CD8+T细胞中的分布。我们设想使用单一核心将提高实验方法的严密性和可重复性，增加协同效应研究人员和实验室之间的数据交换，并能够直接比较结果数据集。新一代这些数据集将使BCB核心和个别项目能够实现阐明组织驻留CD8+T细胞的分子异质性、转录和表观遗传调控及其功能在急性和慢性感染的背景下。好了！