Biomimetic Matrix-Based Multiphasic System for Rotator Cuff Repair

用于肩袖修复的仿生基质多相系统

• 批准号: 10223193
• 负责人: Su Chin Heo
• 金额: $ 17.34万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10223193
• 关键词: 3-Dimensional; Address; Animal Model; Animals; Architecture; Area; Biochemical; Biocompatible Materials; Biological; Biological Assay; Biomimetics; Caliber; Cells; Cellular Metabolic Process; Chemicals; Citrates; Clinical; Clinical Trials; Coculture Techniques; Complex; Cues; Data; Development; Devices; Disease; Engineering; Environment; European Union; Evaluation; Extracellular Matrix; Failure; Fiber; Formulation; Future; Histologic; Hyaluronic Acid; Hydroxyapatites; Implant; In Vitro; Injury; Lead; Mechanics; Metabolic; Modeling; Modulus; Molecular; Natural regeneration; Nutrient; Operative Surgical Procedures; Osteoblasts; Pain; Patients; Performance; Phase; Phenotype; Polymers; Procedures; Process; Production; Property; Rattus; Reference Values; Regenerative engineering; Rotator Cuff; Structure; Supporting Cell; Surgical sutures; System; Techniques; Tendon Injuries; Tendon structure; Time; Tissue Engineering; Tissues; Translational Research; Translations; United States; Vascularization; Work; base; bone; calcification; candidate selection; cell behavior; clinical implementation; clinical practice; commercialization; cost; design; healing; improved; in vivo; innovation; migration; musculoskeletal injury; nanofiber; novel; prevent; repair model; repaired; rotator cuff injury; rotator cuff tear; scaffold; tissue regeneration

Project Summary Rotator cuff tendon injury is a very most common musculoskeletal injury, with over 3 million injuries being treated in the United States and European Union each year. These injuries have limited healing and repair potential due to poor vascularization, preventing adequate delivery of nutrients and tendon cells to produce new tissue. Recently, many tissue engineering approaches have been introduced to repair rotator cuff injuries. However, not many products have succeeded due to the lack of the currently available biomaterials. To further development in this area and overcome limitations with current devices, we propose a biomimetic matrix based-multiphasic scaffold designed to be used in conjunction with existing suture anchors to enhance repair and regeneration of the rotator cuff enthesis. In this proposal, further, we will perform in vitro evaluations of cytocompatibility of the scaffolds, and evaluate the in vivo performance of the scaffolds using a small animal rotator cuff repair model. Aim 1 will determine optimal multiphasic scaffold parameters to promote zone- specific enthesis tissue formation in rotator cuff. Aim 2 will evaluate the biological performances of zone- specific multiphasic scaffolds using a co-culture model and an animal rotator cuff repair model. This data will provide valuable references for our future design of a large animal model for translational research and commercialization. Finally, this study will advance a novel multiphasic biomaterial scaffold towards clinical implementation for treatment of rotator cuff tears with the potential for rapid translation to clinical trials and practice.

项目摘要 肩袖肌腱损伤是一种非常常见的肌肉骨骼损伤， 每年在美国和欧盟接受治疗。这些损伤的愈合和修复有限 由于血管化不良，阻止了营养物质和肌腱细胞的充分输送， 新组织。近年来，许多组织工程学方法被用于肩袖损伤的修复。 然而，由于缺乏目前可用的生物材料，没有多少产品获得成功。进一步 在这一领域的发展和克服现有设备的局限性，我们提出了一种仿生矩阵 基于多相支架，设计与现有缝线锚钉结合使用，以增强修复 和肩袖附着点的再生。在本提案中，我们将进一步进行体外评价， 支架的细胞相容性，并使用小动物评价支架的体内性能 肩袖修复模型。目的1将确定最佳的多相支架参数，以促进区域- 在肩袖中形成特定附着点组织。目的2评价区带的生物学性能 使用共培养模型和动物肩袖修复模型的特定多相支架。这些数据将 为我们今后设计转化研究的大型动物模型提供了有价值的参考， 商业化最后，本研究将推动一种新型多相生物支架材料走向临床 用于治疗肩袖撕裂的实施，具有快速转化为临床试验的潜力， 实践

项目成果

3D cell aggregate printing technology and its applications.

• DOI: 10.1042/ebc20200128
• 发表时间: 2021-07
• 期刊: Essays in biochemistry
• 影响因子: 6.4
• 作者: Seunggyu Jeon;Se-Hwan Lee;Saeed B Ahmed;Jonghyeuk Han;S. Heo;Hyunwook Kang